Microbiology Lab Syllabus for VTU BE/B.Tech Civil Engineering third sem complete syllabus covered here. This will help you understand complete curriculum along with details such as exam marks and duration. The details are as follows.
| Subject Code | 15BTL38 | IA Marks | 20 |
|---|---|---|---|
| Number of Lecture Hours/Week | 01Hr Tutorial (Instructions) + 02 Hours Laboratory | Exam Marks | 80 |
| Exam Hours | 3 |
CREDITS – 02
Course objectives:
| Laboratory Experiments: | Revised Bloom’s Taxonomy (RBT) Level |
|---|---|
| 1. Study of Laboratory Instruments | L5,L4 |
| 2. Media preparation, Preparation of plates and tubes. | L2,L3,L4 |
| 3. Pure culture techniques (Streak, pour and spread – plates) | L1,L2,L3,L4,L5 |
| 4. Enumeration of microbes by Plate count and haemo-cytometer. | L2,L4,L5,L6 |
| 5. Determination of size of cell or fungal spores by Micrometry | L2,L3,L5 |
| 6. Gram staining, Capsule staining, and endospore and flagella staining | L2,L3,L4 |
| 7. Staining of fungi | L2,L3,L4 |
| 8. Characterization of bacteria by Biochemical Tests: IMViC, Starch hydrolysis, carbohydrate fermentation, Catalase, Urease, hydrogen sulphide, Nitrate reduction. | L1,L2,L3,L4,L5,L6 |
| 9. Isolation of actinomycetes and rhizobium and their identification | L1,L2,L3,L4,L5 |
| 10. Determination of bacterial motility by hanging drop technique. | L1,L2,L3,L4 |
| 11. Growth curve studies. | L1,L2,L3,L4,L5 |
| 12. Antibiotic sensitivity tests | L1,L2,L3,L4,L5 |
Course outcomes: On the completion of this laboratory course, the students will be able to:
- Use different laboratory equipment and instruments such as Microscope, Laminar Air Flow Station, Autoclave, oven, incubators.
- Prepare the media and use for the cultivation of the microorganisms.
- Perform laboratory experiments for the isolation, identification and characterization of microorganisms.
- Carry-out experiments for the enumeration, staining and control
Graduate Attributes (as per NBA)
- Problem Analysis.
- Design/Development of solutions.
- Professional ethics
- Societal and environmental concern.
- Modern tool usage.
Conduct of Practical Examination:
- All laboratory experiments are to be included for practical examination.
- Students are allowed to pick one experiment from the lot.
- Strictly follow the instructions as printed on the cover page of answer script for breakup of marks.
- Change of experiment is allowed only once and 15% Marks allotted to the procedure part to be made zero.
Reference Book:
- Microbiology: A Lab Manual by Cappuccino Pearson education, 2007
- Lab Math by Dany Spencer Adams, IK Intl. Pub house.
- Lab Ref by Jaine Roskams& Linda Rodgers IK Intl.Pub house.
- Case-Microbiology: An Introduction by Gerard J. Tortora, Berdell R. Funke, Christine L. 11thEdition- Pearson publications.
- Laboratory Manual Of Microbiology And Biotechnology by Aneja K.R. Medtec, 2014
For all other BE/B.Tech 3rd Sem Subject syllabus do follow VTU 3rd Sem BE / B.Tech Syllabus CBCS (2015-16) Scheme for Biotechnology syllabus Group.
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Briefly explain about the experiments which we can easily understand