Lab To Industrial Scaling detail syllabus for Biotechnology (BT), 2017 scheme is taken from VTU official website and presented for VTU students. The course code (17BT743), and for exam duration, Teaching Hr/week, Practical Hr/week, Total Marks, internal marks, theory marks, duration and credits do visit complete sem subjects post given below.
For all other bt 7th sem syllabus for be 2017 scheme vtu you can visit BT 7th Sem syllabus for BE 2017 Scheme VTU Subjects. For all other Professional Elective-3 subjects do refer to Professional Elective-3. The detail syllabus for lab to industrial scaling is as follows.
Course Objectives:
This objective of this course is to
- Identify fermentation as a basic biochemical process, types of fermentation & fermentation products with medium raw materials, sterilization, optimization, inoculum preparation & economics of fermentation.
- Describe the upstream & downstream processes used in fermentation industry with special emphasis on scale up of media, inoculum, aeration & agitation.
- Sketch the various products of fermentation processes useful for the health of mankind.
- Describe the on-line & off-line analysis of fermentation data for the process parameters (physical, chemical & biological) like temperature, pressure, pH, aeration, agitation, fluid rheology, foam & bacterial growth.
- Explain the various parameters to be considered while designing a fermenter & its ancillary equipment’s.
Module 1
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Module 2
RAW MATERIALS, STERILIZATION & PREPARATION OF INOCULUM: Selection of typical raw materials, Different media for fermentation, Optimization of media, Different sterilization methods – batch sterilization, continuous sterilization, filter sterilization, Oxygen requirement. Inoculum preparation from laboratory scale to pilot scale and large scale fermentation, maintenance of aseptic condition.
Module 3
FERMENTER DESIGN, AREATION & AGITATION: Basic structure of fermenter body construction. Description of different parts of fermenter aseptic conditions. Different types of fermenters. Supply of oxygen, fluid rheology, factors affecting aeration and agitation. Scale up and scale down of aeration and agitation.
Module 4
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Module 5
INDUSTRIAL OPERATIONS
Recovery and purification of products, Use of filtration and centrifugation, cell disruption, chemical methods, extraction, chromatographs methods, drying and crystallization, membrane process. Effluent treatment: Disposal methods, treatment process, aerobic and anaerobic treatment, byproducts. Economic aspects: Fermentation as a unit process, economy of fermentation, market potential. Legalization of products like antibiotics and recombinants.
Course Outcomes:
After studying this course, students will be able to:
- Understand molecular and cellular aspects of bacterial and viral diseases
- Explain mechanisms involved in microbial pathogenesis and host responses
- Comprehend mechanism of action of antimicrobial drugs and vaccines
- Carry out research involving molecular microbiology and microbial genomics.
Reference Books:
- Fermentation & Enzyme Technology by D.I.C. Wang et.al., Wiley Eastern.
- Purifying Proteins for Proteomics by Richard J Simpson, IK International.
- BIOSEPARATIONS: Science and Engineering by ROGER G HARRISON, Oxford Publications.
Text Books:
- Downstream Process Technology – A new horizon in Biotechnology by Nooralabetta Krishna Prasad, PHI Learning Private Limited.
- Bioseparation – Downstream processing for biotechnology by Belter P.A., Cussier E. and Wei Shan Hu., Wiley Interscience Pub.
- Separation Processes in Biotechnology by Asenjo J. et al., Marcel Dekker Publications.
- Bioseparations by Belter P.A. and Cussier E., Wiley.
- Product Recovery in Bioprocess Technology – BIOTOL Series,VCH.
- Cellular Microbiology – Pascale Cossart, Patrice Boquet, Staffan Normark, and Rino Rappuoli), 2nd Edition, ASM Press
- Microbiology: Diversity, Disease, and the Environment (Abigail A. Salyers, Dixie D. Whitt) Fitzgerald Scince Press.
- Bacterial Invasion of Host Cells – Edited by Richard J. Lamont, Cambridge University Press
For detail syllabus of all other subjects of BE Bt, 2017 regulation do visit Biotech 7th Sem syllabus for 2017 Regulation.
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