Genetic Engineering & Applications BT 5th Sem Syllabus for VTU BE 2017 Scheme

Genetic Engineering & Applications detail syllabus for Biotechnology (Bt), 2017 scheme is taken from VTU official website and presented for VTU students. The course code (17BT52), and for exam duration, Teaching Hr/week, Practical Hr/week, Total Marks, internal marks, theory marks, duration and credits do visit complete sem subjects post given below.

For all other bt 5th sem syllabus for be 2017 scheme vtu you can visit BT 5th Sem syllabus for BE 2017 Scheme VTU Subjects. The detail syllabus for genetic engineering & applications is as follows.

Course Objectives:

This course will enable students to learn

• about rDNA technology, vectors and enzymes used in genetic engineering.
• acquire the knowledge of specific techniques like PCR, NA hybridization & libraries.
• about various gene transfer techniques, applications of transgenic plants & animals and importance of gene therapy.

Module 1

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Module 2

NUCLEIC ACID HYBRIDIZATION, AMPLIFICATION & CONSTRUCTION OF LIBRARIRES: Methods of nucleic acid detection, polymerase chain reaction (PCR), variants of PCR and applications, methods of nucleic acid hybridization, Southern, Northern & Western hybridization techniques & applications. Isolation of nucleic acids (DNA & RNA). Isolation of plasmids, construction of genomic and cDNA libraries, purification, screening and preservation.

Module 3

METHODS OF GENE/DNA TRANSFER: Overview & classification of gene transfer techniques in plants, animals and microbes -Transformation, stable & transient transformation, transfection, electroporation, microinjection, liposome mediated gene transfer, transfection of DNA by calcium phosphate coprecipitation, gene gun method. Agrobacterium-mediated gene transfer in plants – Ti & Ri plasmids: structure and functions, Ti plasmid based vectors – advantages, disease control of Agrobacterium tumefaciens. Chloroplast transformation & its applications.

Module 4

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Module 5

OTHER APPLICATIONS & GENE THERAPY
Microbial biotechnology – Genetic manipulation, engineering microbes for the production of antibiotics, enzymes, Insulin, growth hormones, monoclonal antibodies, clearing oil spills. Introduction to gene therapy. Methods of Gene therapy. Gene targeting and silencing. Gene therapy in the treatment of cancer, SCID, muscular dystrophy, respiratory disease (emphysema), cystic fibrosis. Challenges & future of gene therapy.

Course Outcomes:

After studying this course, students will be able to:

• Explain & compare the different vectors & enzymes used in the construction of recombinant DNA in Genetic engineering
• Choose& explain specific techniques like PCR, Blotting & construction of libraries
• Differentiate between & learn the different gene/DNA transfer techniques
• Outline the various methods of producing transgenic organisms and sub-divide/summarize the applications of genetic engineering for the welfare of mankind & society

Reference Books:

1. Molecular Biotechnology
2. Genetic Engineering
3. Recombinant DNA
4. Vectors
5. Principles of Microbe & Cell Cultivation
6. Basic Biotechnology
7. Applied Bioremediation and Phytoremediation

Text Books:

1. The Molecular pathology of human disease- From genetics to gene therapy
2. Introduction to Genetic Engineering
3. DNA Science: A first course
4. Industrial Microbiology
5. Molecular Biotechnology- Principles and Applications of recombinant DNA
6. Industrial Microbiology
7. Industrial Microbiology- An introduction
8. Principles of gene manipulation- An introduction to genetic engineering
9. Genes VIII

As per choice Based Credit System (CBCS) scheme]
IMMUNOTECHNOLOGY
Course Code 17BT53 CIE Marks 40
Number of Lecture Hours/week 04 SEE Marks 60
Total Number of Hours 50 (
per Module) Exam Hours 03
CREDITS – 04

Course Objectives:

This course will enable students to

• Learn the underlying concepts of molecular and cellular mechanisms involved in the development and regulation of the immune response
• Describe the cause and treatment for Immune System Pathologies and Dysfunctions.
• Learn the importance techniques of Immunodiagnosis.

Module 1

IMMUNE SYSTEM : Cells of immune system, innate and acquired immunity, primary and secondary lymphoid organs, Antibodies and their structure, structure and function of immunoglobulins; immunoglobulin classes and subclasses (allotypes, idiotypes and anti-idiotypic antibodies)antigens: chemical and molecular nature, clonal selection theory, humoral and cellular immunity.

Module 2

HUMORAL AND CELL MEDIATED IMMUNITY: B-lymphocytes and their activation, antibody genes and generation of diversity, production of monoclonal antibodies, polyclonal antibodies and applications, cytokines, Thymus derived lymphocytes (T cells) – their ontogeny and types, MHC Complex, antigen presenting cells (APC), Activation of T-cells, antigen processing and presentation, Major histocompatibility Complex- MHC Class I and II molecules.

Module 3

INFECTION AND IMMUNITY: Injury and inflammation, immune response to infections: immunity to viruses, bacteria, fungi and parasites, complement, Hypersensitivity, Immunodeficiencies, Criteria and causes of autoimmune disorders, types of autoimmunity, rheumatoid arthritis, animal model.

Module 4

TRANSPLANTATION AND TUMOR IMMUNOLOGY: Relationship between donor and recipient, role of MHC molecules in allograft rejection, bone marrow and haematopoietic stem cell transplantation, tumor antigens, categories of tumor antigens, tumor immunoprophylaxis. immunosupression, tolerance, resistance and immunization, vaccines.

Module 5

MOLECULAR IMMUNOLOGY & IMMUNODIAGNOSIS Catalytic antibodies, application of PCR technology to produce humanized antibodies (Single chain fragment variable), immunotherapy with genetically engineered antibodies, Brief mention about stem cells and applications to immunology, Antigen antibody interaction – Precipitation reactions, Agglutination reactions, Blood typing, A, B, ABO & Rh, principles and applications of ELISA,
Radio Immuno Assay (RIA), western blot analysis, immuno-electrophoresis, Immunofluorescence, chemiluminescence assay, fluorescence activated cell sorting analysis.

Course Outcomes:

After studying this course, students will be able to:

• Outline the molecular and cellular mechanisms involved in the development and regulation of the immune response,
• Describe the cause, challenges and treatment for Immune System Pathologies and Dysfunctions.
• Apply the major immunological laboratory techniques and their application to both clinical analysis and experimental research

Reference Books:

1. Essential Immunology by Roitt I. Blackwell Scientific Publications, Oxford.
2. Molecular Immunology By Benjamini E.
3. Immunology a short course by Benjamini E. and Leskowitz S. Wiley Liss.
4. The Immune System by Peter Parham, Garland Science.
5. Understanding Immunology by Peter Wood, Pearson Education.

Text Books:

1. Immunology – an Introduction by Tizard, Thomson.
2. Immunology by J Kuby, WH Freeman.
3. Immunology & Immunotechnology by Ashim K Chakravarthy, Oxford University Press.
4. Immundiagnostics by S C Rastogi, New Age International

For detail syllabus of all other subjects of BE Bt, 2017 scheme do visit Biotech 5th Sem syllabus for 2017 scheme.

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