Enzyme Technology & Biotransformation detail syllabus for Biotechnology (Bt), 2017 scheme is taken from VTU official website and presented for VTU students. The course code (17BT63), and for exam duration, Teaching Hr/week, Practical Hr/week, Total Marks, internal marks, theory marks, duration and credits do visit complete sem subjects post given below.
For all other bt 6th sem syllabus for be 2017 scheme vtu you can visit Biotech 6th Sem syllabus for BE 2017 Scheme VTU Subjects. The detail syllabus for enzyme technology & biotransformation is as follows.
Course Objectives:
This course will enable students to
- To define the enzyme and its classification, catalytic action.
- To understand the mechanism of enzyme action, purification of enzymes, catalytic action of enzymes, kinetics of enzyme catalyzed reactions
Module 1
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Module 2
ENZYMES AND CO- ENZYMES: Acetylcholinesterase, angiotensin converting enzyme (ACE), ACE Inhibitors, HMG Co A reductase inhibitors, pseudocholinesterase, 5′-nucleotidase (5NT), glucose-6-phosphate dehydrogenase (GPD), CKisoforms, immunoreactive trypsinogen (IRT) and chymotrypsin; amylase isoenzymes. Mechanism of coenzymes (NAD/NADP, FAD/FADH2, PLP, Coenzyme A, TPP, Biotin).
Module 3
ENZYMATIC TECHNIQUES : Enzyme and isoenzyme measurement methods with two examples (fixed incubation and kinetic methods); Methods for investigating the kinetics of Enzyme catalysed reactions -Initial velocity studies, rapid-reaction techniques. Standardization and optimization methods, stability of enzymes. Techniques of enzyme immobilization; kinetics of immobilized enzymes, effect of solute, partition & diffusion on the kinetics of immobilized enzymes, design and configuration of immobilized enzyme reactors; applications of immobilized enzyme technology, Economic argument for immobilization.
Module 4
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Module 5
APPLICATIONS :
Importance of enzymes in diagnostics, Enzyme pattern in diseases like Myocardial infarctions (SGOT, SGPT & LDH). Isoenzymes (CK, LD, ALP). Use of isozymes as markers in cancer and other diseases. Enzymes in immunoassay techniques. Therapeutic enzymes. Inborn errors of metabolism. Enzymes used in detergents, use of proteases in food, leather and wool industries; methods involved in production of glucose syrup from starch (using starch hydrolyzing enzymes), production of maltose and sucrose, glucose from cellulose, uses of lactase in dairy industry, glucose oxidase and catalase in food industry; Restriction enzymes and DNA ligases.
Course Outcomes:
After studying this course, students will be able to:
- Define enzymes and its catalytic action, mechanism & kinetics with few examples.
- Explain the various techniques involved in the extraction and utilization of enzymes in biotransformation.
Reference Books:
- Enzyme Technology by Messing, Wiley, New York
- Purifying Proteins for Proteomics by Richard J Simpson, IK International.
- Proteins and Proteomics by Richard J Simpson, IK International.
- Enzymes by Dixon and Webb, IRL Press.
- Principles of Enzymology for technological Applications by Butterworth Heinemann, Oxford University Press.
- Biocatalyst for Industry by J.S. Dordrick, Plenum press, New York.
- Enzymes in Industry: Production and Applications by W. Gerhartz VCH Publishers.
- Fundaments of Enzymology by Prices and Stevens, Oxford Press.
Text Books:
- Fundaments of Enzymology by Nicholas C Price and Stevens, Oxford Press.
- Enzymes – Biochemistry, Biotechnology, Clinical Chemistry by Trevor Palmer, Horwood Publishing Limited.
- Biotransformations in Organic Chemistry by Kurt Faber, Springer Berlin Heidelberg.
- Enzymes in Industry: Production and Applications by W. Gerhartz, VCH Publishers.
- Enzyme Technology by M.F. Chaplin and C. Bucke, Cambridge Press.
For detail syllabus of all other subjects of BE Biotech, 2017 scheme do visit Biotech 6th Sem syllabus for 2017 scheme.
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