Cell Culture Techniques BT 6th Sem Syllabus for VTU BE 2017 Scheme (Professional Elective-2)

Cell Culture Techniques detail syllabus for Biotechnology (BT), 2017 scheme is taken from VTU official website and presented for VTU students. The course code (17BT653), and for exam duration, Teaching Hr/week, Practical Hr/week, Total Marks, internal marks, theory marks, duration and credits do visit complete sem subjects post given below.

For all other bt 6th sem syllabus for be 2017 scheme vtu you can visit BT 6th Sem syllabus for BE 2017 Scheme VTU Subjects. For all other Professional Elective-2 subjects do refer to Professional Elective-2. The detail syllabus for cell culture techniques is as follows.

Course Objectives:

The objectives of this course is to educate students about the fundamental concepts of plant, animal and microbial cell system, their differences, related applications, thus preparing them to meet the challenges of the new and emerging areas of biotechnology industry.

Module 1

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Module 2

MEDIA AND REAGENTS : Types of cell culture media for plants, animals and microbial cells; Ingredients of media; Physiochemical properties; Buffers; Oxygen; Osmolarity; Temperature; Balance salt solutions; Antibiotics, growth supplements; Conditioned media; Other cell culture reagents; Preparation and sterilization of cell culture media and other reagents.

Module 3

ANIMAL CELL CULTURES TECHNIQUES: History of animal cell culture; Different tissue culture techniques; Types of primary culture; Chicken embryo fibroblast culture; Chicken liver and kidney culture; Secondary culture; Trypsinization; Cell separation; Continuous cell lines; Suspension culture; Organ culture; Behaviour of cells in culture conditions: division, growth pattern, metabolism of estimation of cell number; Development, Characterization and maintenance of cell lines, Cryopreservation; Commercial scale production of animal cells, stem cells and their application; Application of animal cell culture for in vitro testing of drugs; Testing of toxicity of environmental pollutants in cell culture.

Module 4

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Module 5

MICROBIAL CELL CULTURE TECHNIQUES:
Microbial Cell Isolation, Culture maintenance. Isolation of pure-colonies. Bacterial titre estimation. Growth curve. Culture characterization. Auxotroph culture isolation. Biochemical characterization. Antibiotic sensitivity. Bacterial recombination, replica plating technique. Preservation of microbial products. Production of antibiotics. Enumeration and screening of novel microbial secondary metabolites, strain improvement, Use of microbes in industrial waste treatment. Microbial leaching.

Course Outcomes:

After studying this course, students will be able to:

• Differentiate between the various sources of cells to be used in cell culture techniques
• Correlate between different biological samples and understand the importance of different media in tissue culture
• Comprehend the applications of plant, animal and microbial cell culture in industry, healthcare and environment.

Reference Books:

1. Plant Tissue Culture by SATHYANARAYANA BN, IK Intl. Publishers
2. Plant Molecular biology by D. Grierson & S.N. Covey Blackie, London.
3. Animal Cell biotechnology by R.E. Spier and J.B. Griffiths, Academic press.
4. Living resources for Biotechnology, Animal cells by A. Doyle, R. Hay and B.E. Kirsop, Cambridge University Press.
5. Fermentation & Enzyme Technology by D.I.C. Wang et.al., Wiley Eastern.
6. Principle of Microbe & Cell Cultivation by SJ Prit, Blackwell Scientific co.
7. Animal cell culture Techniques by Ian Freshney, Wiley-Liss.
8. Animal Cell Culture – Practical Approach BY Ed. John R.W. Masters, 3rd Edition, Oxford University Press.
9. Animal Cell Culture Techniques BY Ed. Martin Clynes,. Springer

Text Books:

1. Plant Cell Culture: A Practical Approach by R.A. Dixon & Gonzales, IRL Press.
2. Experiments in Plant Tissue Culture by John H. Dodds & Lorin W. Robert.
3. Plant tissue Culture: Theory and Practice by S.S. Bhojwani and M.K. Razdan, Elsevier.
4. Animal Biotechnology by Murray Moo-Young, Pergamon Press, Oxford.
5. Microbial Biotechnology by Alexander N Glazer, Hiroshi Nikaido, W H Freeman & Company.
6. Animal Cell Technology by Asok Mukhopadyay, IK Intl. Ltd.

For detail syllabus of all other subjects of BE Biotech, 2017 regulation do visit Biotech 6th Sem syllabus for 2017 Regulation.

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