Genetic Engineering & Applications detailed Syllabus for Biotechnology Engineering (BIOTECH), 2018 scheme has been taken from the VTUs official website and presented for the VTU students. For Course Code, Subject Names, Teaching Department, Paper Setting Board, Theory Lectures, Tutorial, Practical/Drawing, Duration in Hours, CIE Marks, Total Marks, Credits and other information do visit full semester subjects post given below. The Syllabus PDF files can also be downloaded from the official website of the university.
For all other VTU BIOTECH 5th Sem Syllabus for BE 2018 Scheme, do visit VTU BIOTECH 5th Sem Syllabus for BE 2018 Scheme Subjects. The detailed Syllabus for genetic engineering & applications is as follows.
Course Objectives:
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Module 1
VECTORS & ENZYMES IN GENETIC ENGINEERING: Vectors in rDNA technology, salient features of vectors, types of vectors-plasmids, cosmids, phagemids and viruses. Construction of rDNA& vectors (BAC, Blue script and YAC). Exonucleases and Restriction Endonculeases: classification, mode of action. Enzymes in modification-Polynucleotide phosphorylase, DNase, Methylases, phosphatases, polynucleotide Kinase, Ligases, RNase and their mechansim of action
Module 2
NUCLEIC ACID HYBRIDIZATION, AMPLIFICATION & CONSTRUCTION OF LIBRARIES: Methods of nucleic acid detection, polymerase chain reaction (PCR), variants of PCR and applications, methods of nucleic acid hybridization, Southern, Northern & Western hybridization techniques & applications. Isolation of nucleic acids (DNA & RNA). Isolation of plasmids, construction of genomic and cDNA libraries, purification, screening and preservation
Module 3
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Module 4
TRANSGENIC SCIENCE IN GENETIC IMPROVEMENT Transgenic science in plant improvement, biopharming-plants as bioreactors, transgenic crops for increased yield, resistance to biotic and abiotic stresses. Techniques of gene mapping in plants. Marker-assisted selection and breeding for improvement. Transgenic science for animal improvement, biopharming-animals as bioreactors for recombinant proteins, Gene mapping in farm animals. Marker-assisted selection and genetic improvement of livestock.
Module 5
OTHER APPLICATIONS & GENE THERAPY Microbial biotechnology-Genetic manipulation, engineering microbes for the production of antibiotics, enzymes, Insulin, growth hormones, monoclonal antibodies, clearing oil spills. Introduction to gene therapy. Methods of Gene therapy. Gene targeting and silencing. Gene therapy in the treatment of cancer, SCID, muscular dystrophy, respiratory disease (emphysema), cystic fibrosis. Challenges & future of gene therapy.
Course Outcomes:
For the complete Syllabus, results, class timetable, and many other features kindly download the iStudy App
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Question paper pattern:
- The question paper will hav
- Each full question will be f
- There will be two full quest
- Each full question will have
- The students will have to an e ten full questions carryin r 20 marks. ions (with a maximum of fo sub- question covering all iswer five full Questions, se equal marks. ur sub- questions) from each module. the topics under a module. lecting one full question from each module.
Text Books:
- Principles of Gene Manipulation and Genomics S.B. Primrose and R. M. Twyman Blackwell Science Publications 7th edition 2006
- Gene Cloning and DNA Analysis: An Introduction T A Brown Wiley-Blackwell Publications. (6th edition)
Reference Books:
For the complete Syllabus, results, class timetable, and many other features kindly download the iStudy App
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For detail Syllabus of all other subjects of BE 5th Sem Biotechnology Engineering, visit (BIOTECH) 5th Sem Syllabus Subjects.
For all (CBSE & Non-CBSC) BE results, visit VTU BE all semester results direct links.