Bioanalytical Techniques detailed Syllabus for Biotechnology Engineering (BIOTECH), 2018 scheme has been taken from the VTUs official website and presented for the VTU students. For Course Code, Subject Names, Teaching Department, Paper Setting Board, Theory Lectures, Tutorial, Practical/Drawing, Duration in Hours, CIE Marks, Total Marks, Credits and other information do visit full semester subjects post given below. The Syllabus PDF files can also be downloaded from the official website of the university.
For all other VTU BIOTECH 5th Sem Syllabus for BE 2018 Scheme, do visit VTU BIOTECH 5th Sem Syllabus for BE 2018 Scheme Subjects. The detailed Syllabus for bioanalytical techniques is as follows.
Course Objectives:
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Module 1
INTRODUCTION: Extraction, Pre-treatment, Stabilization and preparation methods of bioproducts for analysis.Electrophoretic Techniques: Principle, equipment and process, Agarose gel electrophoresis, gradient electrophoresis, horizontal and vertical gel electrophoresis, electrophoresis techniques, isoelectric focusing, capillary electrophoresis and application of electrophoresis in analyzing macromolecules.Chromatography-principles, instruments and practice, adsorption, reverse phase, ion exchange, size exclusion, hydrophobic interaction, bioaffinity and pseudo affinity chromatographic techniques
Module 2
CHROMATOGRAPHY AND PURIFICATION: Chromatography: Classification of chromatographic techniques and their principles, Theory of chromatography, band broadening, rate and plate theory factors responsible for separation. Column chromatography, TLC, Paper chromatography. Liquid Chromatography and HPLC: Instrumentation, pumps, solvent delivery system, isocratic and gradient programming modes, sample introduction system, columns, detectors, reversed phase and normal phase chromatography. Gas Chromatography: Instrumentation, carrier gas supply, injectors, columns, packed and capillary columns, column oven and temperature programming, different detectors. Introduction to hyphenated techniques in chromatography, GC-MS and LC-MS.Cell fractionation and flow cytometry
Module 3
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Module 4
SPECTROMETRY: Ion sources sample introduction-mass analyzers and ion detectors-bimolecular mass spectrometry-peptide and protein analysis-carbohydrates and small molecules-specific applications. X-Ray diffraction: Scattering by x- rays-diffraction by a crystal-measuring diffraction pattern-Bragg reflection -unit cell-phase problem-anomalous diffraction-determination of crystal structure-electron and neutron diffraction.
Module 5
INSTRUMENTAL METHODS OF ANALYSIS:
Advanced Microscopy: Morphology and identification of cells using microscopic studies like SEM, TEM and Confocal Microscopy.
EDAX, Elemental mapping, FTIR, UV-Visible spectrophotometer, Nanomechanical Characterization using Nanoindentation, Differential Scanning Calorimeter (DSC), Differential Thermal Analyzer (DTA), Thermo gravimetric Analysis (TGA), TEM, X-ray Photoelectron Spectroscopy (XPS), Electrochemcial Characterization measurements.
Course Outcomes:
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Question paper pattern:
- The question paper will have ten full questions carryin
- Each full question will be for 20 marks.
- There will be two full questions (with a maximum of fo
- Each full question will have sub- question covering all
- The students will have to answer five full questions, se equal marks. ur sub- questions) from each module. the topics under a module. lecting one full question from each module.
Text Books:
- Analytical techniques in biochemistry and molecular biology R. Katoch Springer, New York, 2011
- Biological spectroscopy I. D. Campbell Benjamin/Cummings Pub. Co 1984
- Separation Processes in Biotechnology Asenjo, Juan A. CRC / Taylor & Francis 1990
Reference Books:
For the complete Syllabus, results, class timetable, and many other features kindly download the iStudy App
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For detail Syllabus of all other subjects of BE 5th Sem Biotechnology Engineering, visit (BIOTECH) 5th Sem Syllabus Subjects.
For all (CBSE & Non-CBSC) BE results, visit VTU BE all semester results direct links.