18BT53: Enzyme Technology & Biotransformation BIOTECH Syllabus for BE 5th Sem 2018 Scheme VTU

Enzyme Technology & Biotransformation detailed Syllabus for Biotechnology Engineering (BIOTECH), 2018 scheme has been taken from the VTUs official website and presented for the VTU students. For Course Code, Subject Names, Teaching Department, Paper Setting Board, Theory Lectures, Tutorial, Practical/Drawing, Duration in Hours, CIE Marks, Total Marks, Credits and other information do visit full semester subjects post given below. The Syllabus PDF files can also be downloaded from the official website of the university.

For all other VTU BIOTECH 5th Sem Syllabus for BE 2018 Scheme, do visit VTU BIOTECH 5th Sem Syllabus for BE 2018 Scheme Subjects. The detailed Syllabus for enzyme technology & biotransformation is as follows.

Course Objectives:

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Module 1

INTRODUCTION : Introduction to enzymes, Classification, Sources, Strategies of purification of enzymes, molecular weight determination, Mechanism of enzyme catalysis (Acid-base, Covalent, Metal ion catalysis, Substrate strain & entropy effects), criteria of purity and characterization of enzymes. Advantages of Biocatalyst vs Chemical catalysts, Isolated Enzymes versus whole cell systems, Application of enzymes in different industry

Module 2

ENZYME ASSAY AND CO- ENZYMES: Enzyme and isoenzyme measurement methods with two examples (fixed incubation and kinetic methods); Enzymes in immunoassay techniques, Methods for investigating the kinetics of Enzyme catalyzed reactions -Initial velocity studies, rapid-reaction techniques. Standardization and optimization methods, stability of enzymes. Mechanism of coenzymes (NAD/NADP, FAD/FADH2, PLP, Coenzyme A, TPP, Biotin).

Module 3

Module 4

ENZYME ENGINEERING AND MEDICAL IMPORTANCE : The design and construction of novel enzymes, artificial enzymes, Host Guest Complexation chemistry and enzyme design using steroid templates. In vitro Biotransformation of drugs (hydroxylation of Steroids), Therapeutic enzymes, Acetylcholinesterase and pseudocholinesterase, Angiotensin converting enzyme (ACE) and Inhibitors, HMG Co A reductase inhibitors, glucose-6-phosphate dehydrogenase (GPD), Immunoreactivetrypsinogen (IRT) and amylase isoenzymes.

Module 5

APPLICATIONS : Importance of enzymes in diagnostics, Enzyme pattern in diseases like Myocardial infarctions (SGOT, SGPT & LDH). Isoenzymes (CK, LD, ALP). Use of isozymes as markers in cancer and other diseases. Enzymes used in detergents, use of proteases in food, leather and wool industries; methods involved in production of glucose syrup from starch (using starch hydrolyzing enzymes), production of maltose and sucrose, glucose from cellulose, uses of lactase in dairy industry, glucose oxidase and catalase in food industry;

Course Outcomes:

Question paper pattern:

The question paper will have ten full questions carrying equal marks.
Each full question will be for 20 marks.
There will be two full questions (with a maximum of four sub- questions) from each module.
Each full question will have sub- question covering all the topics under a module.
The students will have to answer five full questions, selecting one full question from each module.

Text Books:

Enzyme Technology Martin Chaplin and Christopher Bucke Cambridge University Press 1990
Enzymes Dixon and Webb Academic Press 2nd Edition 1964
Principles of Enzymology for technological Applications Butterworth Heinemann Oxford University Press 1993

Reference Books:

For detail Syllabus of all other subjects of BE 5th Sem Biotechnology Engineering, visit (BIOTECH) 5th Sem Syllabus Subjects.

For all (CBSE & Non-CBSC) BE results, visit VTU BE all semester results direct links.

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