Cell Culture Techniques detailed Syllabus for Biotechnology Engineering (BIOTECH), 2018 scheme has been taken from the VTUs official website and presented for the VTU students. For Course Code, Subject Names, Teaching Department, Paper Setting Board, Theory Lectures, Tutorial, Practical/Drawing, Duration in Hours, CIE Marks, Total Marks, Credits and other information do visit full semester subjects post given below. The Syllabus PDF files can also be downloaded from the official website of the university.
For all other VTU BIOTECH 4th Sem Syllabus for BE 2018 Scheme, do visit VTU BIOTECH 4th Sem Syllabus for BE 2018 Scheme Subjects. The detailed Syllabus for cell culture techniques is as follows.
Course Objectives:
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Module 1
CELL CULTURE LABORATORY DESIGN AND EQUIPMENTS: Planning, construction and services; Layout; Sterile handling area; Incubation; Hot room; Air circulation; Service bench; Laminar flow; Sterilizer; Incubators; CO2 incubator; Culture Racks, Colony Counters, Refrigerators and freezers; Centrifuge; Inverted stage microscope; Magnetic stirrer; Liquid nitrogen freezers; Slow cooling system for cell freezing; Water bath; Autoclaves and hot air oven; Pipette washers; Water purification system; Fluid handling systems and other equipments; Washing, packing and sterilization of different materials used in plants, animals and microbial cell cultures; Aseptic concepts; Maintenance of sterility; Cell culture vessels
Module 2
MEDIA AND REAGENTS : Types of cell culture media for plants, animals and microbial cells; Ingredients of media; Physiochemical properties; Buffers; Oxygen; Osmolarity; Temperature; Balance salt solutions; Antibiotics, growth supplements; Conditioned media; Other cell culture reagents; Preparation and sterilization of cell culture media and other reagents.
Module 3
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Module 4
PLANT CELL CULTURE TECHNIQUES: Cellular Totipotency, And its Applications. Organogenesis, factors affecting organogenesis. Cyto-differentiation. Somatic Embryogenesis, Synthetic Seeds, Techniques for production of haploids, diploidization, production of double haploids and their Applications. Triploids production-Endosperm culture and Applications. Secondary metabolite production, selection of high yielding lines, elicitation, immobilization of cultures, hairy root culture and biotransformation. Factors affecting secondary metabolites, industrial application of secondary metabolites. Molecular farming.
Module 5
MICROBIAL CELL CULTURE TECHNIQUES: Auxotroph isolation-replica plating technique, Screening Preservation of microbial products. Production of antibiotics. Enumeration and screening of novel microbial secondary metabolites, strain improvement, Use of microbes in industrial waste treatment. Microbial leaching.
Course Outcomes:
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Question paper pattern:
- The question paper will have ten full questions carrying equal marks.
- Each full question will be for 20 marks.
- There will be two full questions (with a maximum of four sub- questions) from each module.
- Each full question will have sub- question covering all the topics under a module.
- The students will have to answer five full questions, selecting one full question from each module.
Text Books:
- Plant Cell Culture: A Practical Approach R.A. Dixon & Gonzales IRL Press 1994
- Culture of animal cells-A manual of basic technique and specialized applications R. Ian Freshney Wiley Blackwell publishers 1983
- Microbial Biotechnology Alexander N Glazer, Hiroshi Nikaido W H Freeman & Company 1995
Reference Books:
For the complete Syllabus, results, class timetable, and many other features kindly download the iStudy App
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For detail Syllabus of all other subjects of BE 4th Sem Biotechnology Engineering, visit (BIOTECH) 4th Sem Syllabus Subjects.
For all (CBSE & Non-CBSC) BE results, visit VTU BE all semester results direct links.