Bioseparation and Downstream Processing detail syllabus for Biotechnology (Biotechnology), 2021-22 scheme is taken from AKTUs official website and presented for the AKTU B.Tech students. For the course code (KBT751B), exam duration, teaching hr/week, practical hr/week, total marks, internal marks, theory marks, duration, credits, and other details do visit complete semester subjects post given below.
For the Biotechnology 7th Sem Syllabus for AKTU B.Tech 2021-22 Scheme you can visit Biotechnology 7th Sem 2021-22 Scheme. For the Departmental Elective Labs-I scheme of Biotechnology 7th Sem 2021-22 regulation do refer to Departmental Elective Labs-I Biotechnology 7th Sem scheme. The detail syllabus for bioseparation and downstream processing is as follows.
PreRequisites:
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Course Objectives:
- The major objective of this course is to impart in students the skills to operate bioprocesses for production of various Bio-products
- This course is formulated to teach various methods of product separation, isolation and purification To teach the construction of genomic c-DNA libraries, cloning and strain improvement
Course Outcomes:
For the complete syllabus, results, class timetable, and many other features kindly download the iStudy App
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Reference Books:
- Raja Ghosh, ‘Principles of Bioseparations Engineering’, WorldScientific Publishing 2006
- Ladisch. M. R, “Bioseparation Engineering: Principles, Practice and Economics”, John Wiley & sons, New York. 2001
- Asenjo.J.M, “Separation processes in Biotechnology” Marcel Dekker Inc. 1993
- Bailey & oils, Biochemical Engg. Fundamentals, McGraw-Hill 1990
- Roger G. Harrison, Paul Todd, Scott R. Rudge, Demetri P. Petrides, “Bioseparation science and Engineering” Oxford University press. 2003
Unit I
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Unit II
REMOVAL OF INSOUBLES: Cell disruption methods for intracellular products: Physical, chemical and mechanical – Removal of insolubles: Biomass and particulate debris separation techniques – flocculation -sedimentation – centrifugation and filtration methods.
Unit III
ISOLATION OF PRODUCTS: Adsorption: Principles – Langumir -Freundlich isotherms – Extraction: Basics- Batch and continuous, aqueous two-phase extraction – supercritical extraction – in situ product removal -Precipitation: Methods of precipitation with salts – organic solvents and polymers – Membrane based separations: Micro and ultra filtration – theory -design and configuration of membrane separation equipments and its applications.
Unit IV
PURIFICATION OF BIOPRODUCTS: Basic principles of Chromatographic separations: GC-HPLC – gel permeation – ion- exchange -affinity – reverse phase and hydrophobic interaction chromatography – Electrophoretic separation techniques: capillary -isoelectric focusing-2D gel electrophoresis -Hybrid separation technologies: GC-MS and LC-MS.
Unit V
PRODUCT POLISHING: Crystallization: Principles-Nucleation- Crystal growth-Kinetics-Batch crystallizers: Scale-up and design, Drying: Principles-Water in biological solids- Heat and mass transfer- Drying equipments: description and operation-Vacuum shelf – rotary dryer-Freeze dryer-Spray dryer. Biomolecules of Commercial importance Ethanol, citric acid, lysine, steroids, penicillin, dextran, trehalose, subtilisin, chymosin, vitamin B12, hepatitis B vaccine, insulin, erythropoietin, monoclonal antibodies.
For the syllabus of all the subjects of B.Tech Biotechnology 7th Sem, 2021-22 scheme do visit Biotechnology 7th Sem syllabus subjects.
For the complete syllabus, results, class timetable, and many other features kindly download the iStudy App
It is a lightweight, easy to use, no images, and no pdfs platform to make students’s lives easier.