Genomics and Proteomic Lab detail syllabus for Biotechnology (Biotechnology), 2021-22 scheme is taken from AKTUs official website and presented for the AKTU B.Tech students. For the course code (KBT751A), exam duration, teaching hr/week, practical hr/week, total marks, internal marks, theory marks, duration, credits, and other details do visit complete semester subjects post given below.
For the Biotechnology 7th Sem Syllabus for AKTU B.Tech 2021-22 Scheme you can visit Biotechnology 7th Sem 2021-22 Scheme. For the Departmental Elective Labs-I scheme of Biotechnology 7th Sem 2021-22 regulation do refer to Departmental Elective Labs-I Biotechnology 7th Sem scheme. The detail syllabus for genomics and proteomic lab is as follows.
PreRequisites:
Molecular Biology & Biochemistry
Course Objectives:
- To isolate genetic materials from bacterial cells
- To determine and characterization of enzyme kinetics
Course Outcomes:
After completion of this course successfully, the students will be able to:
- Express and purify recombinant protein in bacterial system
- Characterized purified protein through enzymatic activity and kinetics
- Work with publically available genome sequencing data
- Perform genomic DNA and RNA isolation from bacteria and independently setup qRT-PCR
Reference Books:
- Application of DNA microarrays in Biology 2005
- Functional Proteomics 2005
- Proteomic to study genes and genomes 2000
List of Experiments:
- Expression of heterologous protein in bacterial system.
- Detection of expressed protein.
- Purification of recombinant protein.
- Characterization of purified protein using enzymatic activity.
- Determination of kinetic parameters.
- Browsing through various publically available genome databases, retrieving and working with genome data. KEGG genomes, retrieval of genes, proteins and intergenic regions.
- Genomic DNA isolation from bacterial strains and estimation of DNA concentration using Nanodrop, agarose gel electrophoresis.
- RNA isolation from Synechocystis and quality check (RIN number) using Bioanalyser chip, cDNA synthesis and qRT-PCR analysis.
- Demonstration of Agilent’s microarray scanner and DNA microarray protocol.
- Raw data processing, scatter plots, global normalization of data, fold changes and finding differentially expressed genes.
For the syllabus of all the subjects of B.Tech Biotechnology 7th Sem, 2021-22 scheme do visit Biotechnology 7th Sem syllabus subjects.
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