Genetic Engineering detailed syllabus scheme for Biotechnology (BT), 2018 regulation has been taken from the University of Mumbai official website and presented for the Bachelor of Engineering students. For Course Code, Course Title, Test 1, Test 2, Avg, End Sem Exam, Team Work, Practical, Oral, Total, and other information, do visit full semester subjects post given below.
For all other Mumbai University Biotechnology 5th Sem Syllabus 2018 Pattern, do visit BT 5th Sem 2018 Pattern Scheme. The detailed syllabus scheme for genetic engineering is as follows.
Genetic Engineering Syllabus for Biotechnology TE 5th Sem 2018 Pattern Mumbai University
Prerequisites:
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Course Objectives:
- Give insight into the functioning of Recombinant DNA molecules, their constructions, analysis and fine tuning.
- To engineer such molecules for making of difficult bio-molecules.
- This course also gives various ideas and approaches by different schools of thoughts.
Course Outcomes:
By learning this course the students will be able to:
- Understand how recombinant molecules are created analysed with respect to DNA, RNA, and Protein.
- They also will be familiar with the problems they could encounter and how to trouble shoot them.
- They will be able to monitor both in-vitro and in-vivo activity.
- They will be able to suggest more rational approach to solve problem of a living system at a molecular level.
Module 1
Basics of genetic material: DNA structure, topology, Super helical and relaxed molecules. Plasmids- Basic features classification, size and copy number, conjugation & compatibility. Total cell DNA preparation, different methods of plasmid DNA preparation. 06
Module 2
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Module 3
Cloning vectors: Vectors used for Gene-cloning: Plasmids (e.g pUC type, conjugative, Ti etc), Phages (Lambda and M13 type), Cosmids and Phagemids. 07
Module 4
Library construction and recombinant gene expression: Library construction (Genomic and C-DNA type) and Screening for the clone. Foreign gene expression in E. coli, Fusion proteins 06
Module 5
DNA transfer in to cells: Transformation and Transfection, Membrane Fusion and Electroporation, Gene-Gun and Micro-injection 05
Module 6
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Module 7
Antisense and RNA interference Technology and their applications. R-DNA in medicine, e.g. Insulin and Blood clotting factor VIII. 06
Assessment
Internal
- Assessment consists of two tests which should be conducted at proper intervals.
End Semester theory examination
- Question paper will comprise of 6 questions each carrying 20 questions.
- Total 4 questions need to be solved
- Question no.1 will be compulsory based on entire syllabus wherein sub questions can be asked.
- Remaining questions will be randomly selected from all the modules
- Weightage of marks should be proportional to number of hours assigned to each module
Reference Books:
- Molecular Biology of the Cell: Alberts et al.5th. Ed. Garland Publications.
- Genes VIII: Benjamin Levine, Oxford University Press.
- Principle of Gene Manipulations (2004): S.B. Primrose, R.M. Twyman & R. W. Old. Sixth edition. Blackwell Science.
- Gene Cloning and DNA analysis: An Introduction.(2006).T. A. Brown. Blackwell Publishing.
For detail syllabus of all other subjects of Biotechnology (BT) 5th Sem 2018 regulation, visit BT 5th Sem Subjects syllabus for 2018 regulation.