Basic Microbiology detailed Syllabus for Medical Laboratory Technology (ML), K scheme PDF has been taken from the MSBTE official website and presented for the diploma students. For Subject Code, Subject Name, Lectures, Tutorial, Practical/Drawing, Credits, Theory (Max & Min) Marks, Practical (Max & Min) Marks, Total Marks, and other information, do visit full semester subjects post given below.
For all other MSBTE Medical Laboratory Technology 3rd Sem K Scheme Syllabus PDF, do visit MSBTE Medical Laboratory Technology 3rd Sem K Scheme Syllabus PDF Subjects. The detailed Syllabus for basic microbiology is as follows.
Rationale
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Course Outcomes:
Students will be able to achieve & demonstrate the following COs on completion of course based learning
- Identify morphology of bacteria
- Perform various staining techniques and antibiotic sensitivity test
- Prepare culture media following recommended procedure
- Perform biochemical test
- Apply different aseptic techniques following their recommended safety principles
Unit I
Introduction to Bacteriology 1.1 Contributions of Robert Koch and Koch’s postulates 1.2 Compound microscope – diagram, components, uses and care 1.3 Structure of bacterial cell – Capsule, Flagella, Fimbrae, Cell wall 1.4 .Arrangements of bacterial cell with examples Model Demonstration Video Demonstrations Lecture Using Chalk-Board Hands-on Demonstration
Unit II
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Unit III
Culture Media 3.1 Composition, preparation, uses and sterilization of Basal media – Peptone water, nutrient agar and Mueller Hinton agar. 3.2 Composition, Preparation, uses and sterilization of Enriched media- Blood agar, Chocolate agar and Loeffler’s serum slope 3.3 Composition, preparation, uses and sterilization of Selective media – Mac Conkey’s agar, UTI agar, Middlebrook media 3.4 Composition, Preparation, uses and sterilization of Enrichment media- Glucose broth, Nutrient and Bile broth. Video Demonstrations Lecture Using Chalk-Board Hands-on Flipped Classroom
Unit IV
Biochemical Tests 4.1 Principle, media, reagents and procedure of Carbohydrate fermentation test. 4.2 Principle, media, reagents and procedure of Indole test 4.3 Principle, media, reagents and procedure of Methyl red test, VP test 4.4 Principle, media, reagents and procedure of citrate utilization test 4.5 Principle, media, reagents and procedure of catalase test, oxidase test Video Demonstrations Flipped Classroom Lecture Using Chalk-Board Hands-on Collaborative learning
Unit V
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Micro Project / Assignment
Micro project
- Draw a hand sketch of the compound microscope
- Prepare a model of different arrangement of bacteria
- Prepare a Wooden model of Bacterial Cell
- Prepare a Wooden & Electrical Model of Biochemical Test.
- Collect water samples from different sources and observe them under the microscope and record observations
- Prepare a wooden model of microbiology laboratory
Assignment
- Classification & Uses of disinfecting agents.
- Enlist the Gram Positive & Gram Negative Bacteria
- Collect information on the history of microbiology
- Give the Example of Indole ,MR,V.P. Catalase,Oxidase,Citrate Positive Bacteria
- Enlist culture methods used in laboratory
- Enlist and classify antibiotics
- Classification of the culture media based on the composition of media
Laboratory Equipment
- Incubator 11,12,13,14,15,16
- Compound Microscope 3,4,5,1,2
- Laminar Air Flow 3,4,5,12,13,14,15,16,2
- Autoclave 6,7,8,9,10,11,12,13,14,15,16
- Analytical balance 6,7,8,9,10,11,12,13,14,15,16
- Hot air Oven 9,10,11
Learning Materials/Books
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Learning Websites & Portals
- https://micro.magnet.fsu.edu/cells/bacteriacell.html Structure of Bacterial Cell
- https://microbiologyinfo.com/different-size-shape-and-arrang ement-of-bacterial-cells Arrangement of bacterial cell
- https://youtu.be/a6nmy-9uUdw?si=H7FzZSgicFisuAu8 Sterilization & Disinfection
- https://youtu.be/RDXtxa-mee4?si=czg0j1XHKZvFoeyZ Acid fast staining
- https://youtu.be/E4a8g1o72AM?si=kaZC6qksBZz5GKjf Biochemical Test
- https://en.wikipedia.org/wiki/Growth_medium Growth media 0
- https://en.wikipedia.org/wiki/L%C3%B6wenstein%E2%80%93Jensen _medium Lowenstein Jenson media
- https://microbenotes.com/category/biochemical-test/ Biochemical Test
- https://en.wikipedia.org/wiki/Sterilization_(microbiology) Sterilization
- https://en.wikipedia.org/wiki/Disinfectant Disinfection
- https://youtu.be/OBej7rFyN7U?si=3lgPVfUblj1wmoTF Bacterial cell Structure
- https://youtu.be/Yl1EZi0qX1Q?si=Ddjc_kY-R-TfpoEn Culture Media
- https://youtu.be/GOdXRD8R2Zc?si=pUYtRbE8Q58qmBq8 Gram Staining
For detail Syllabus of all other subjects of Medical Laboratory Technology, K scheme do visit Medical Laboratory Technology 3rd Sem Syllabus for K scheme.
For all Medical Laboratory Technology results, visit MSBTE Medical Laboratory Technology all semester results direct links.