15WT54P: Biochemistry and Microbiology Lab Water Tech 5th Sem Syllabus for Diploma DTE Karnataka C15 Scheme

Biochemistry and Microbiology Lab detail DTE Kar Diploma syllabus for Water Technology And Health Science (WH), C15 scheme is extracted from DTE Karnataka official website and presented for diploma students. The course code (15WT54P), and for exam duration, Teaching Hr/week, Practical Hr/week, Total Marks, internal marks, theory marks, duration and credits do visit complete sem subjects post given below. The syllabus PDFs can be downloaded from official website.

For all other water tech 5th sem syllabus for diploma c15 scheme dte karnataka you can visit Water Tech 5th Sem Syllabus for Diploma C15 Scheme DTE Karnataka Subjects. The detail syllabus for biochemistry and microbiology lab is as follows.

Pre-requisites:

Knowledge of Basic Science and Aquatic Chemistry.

Course Objectives:

1. Familiarize with Tools and Equipments used in Biochemistry and Microbiology lab
2. Qualitative Testing of Carbohydrates, Lipids and Proteins.
3. Testing the bacteriological Characteristics of water

Course Outcomes:

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LIST OF GRADED PRACTICAL EXERCISES

Following is the list of experiments to be carried out.

A. INTRODUCTION

1. Familiarization with equipments 03
2. Safety Precautions 03

B. QUALITATIVE TESTS FOR CARBOHYDRATES, LIPIDS AND PROTEINS

1. Detect the presence of Carbohydrates in a solution by Molisch’s test 03
2. Determine the reducing property of carbohydrates by Benedict’s test 03
3. Determine the reducing property of carbohydrates by Barfoed’s test 03
4. Detect the presence of Lipids by Sudan IV 03
5. Detect the presence of Proteins by Bi-uret Test 03

C. BACTERIOLOGICAL CHARACTERISTICS OF WATER

1. Cleaning and Sterilization of Glass Wares Used in Bacteriological tests 03
2. Preparation of Culture Media 06
3. Practicing the sampling techniques for bacteriological tests from different sources of water 06
4. Determination of Bacterial density by standard plate count method 06
5. Determination of Faecal Coliform and Faecal Streptococci by Multiple Tube Fermentation Techniques 18
6. Determination of Faecal Coliform by Membrane filtration technique 06
7. Determination of bacteria by Gram’s Staining 03
8. Bacterial cell immobilization 03

Reference Books:

1. Clair Sawyer, Perry Mccarty and Gene Parkin,”Chemistry for Environmental Engineering and Science”, 5th Edition, Tata McGraw Hill Series in Civil and Environmental Engineering, 2002.
2. APHA, AWWA, WPCF, Washington D.C, “Standard Methods for Examination of Water and Wastewater” 22nd edition.

Suggested List of Student Activities:

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Course Delivery:

The course will be delivered through Demonstration and practices

Note to IA verifier:

The following documents to be verified by CIE verifier at the end of semester

1. Blue books (10 marks)
2. Student suggested activities report for 5 marks
3. Student feedback on course regarding Effectiveness of Delivery of instructions & Assessment Methods.

SCHEME OF VALUATION FOR END EXAMINATION

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EQUIPMENT LIST:

1. Glass wares- Beakers, Pipettes, Burette, Measuring Cylinder, conical flasks, test tubes, Petri dishes, Inoculation loops, Glass Slides, Cover slips.
2. Thermometer
3. Electronic Balance
4. Desiccators
5. Monocular Microscope
6. Colony Counter
7. Hot air Oven
8. Bacteriological Incubator
9. UV-VIS Spectrophotometer
10. UV – Chamber
11. Filtration Apparatus
12. Preservator

For detail syllabus of all other subjects of BE Water Tech, C15 scheme do visit Water Tech 5th Sem syllabus for C15 scheme.

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