15LT32T: Leather Biotechnology and Microbiology Leather Tech 3rd Sem Syllabus for Diploma DTE Karnataka C15 Scheme

Leather Biotechnology and Microbiology detail DTE Kar Diploma syllabus for Leather And Fashion Technology (LT), C15 scheme is extracted from DTE Karnataka official website and presented for diploma students. The course code (15LT32T), and for exam duration, Teaching Hr/week, Practical Hr/week, Total Marks, internal marks, theory marks, duration and credits do visit complete sem subjects post given below. The syllabus PDFs can be downloaded from official website.

For all other leather tech 3rd sem syllabus for diploma c15 scheme dte karnataka you can visit Leather Tech 3rd Sem Syllabus for Diploma C15 Scheme DTE Karnataka Subjects. The detail syllabus for leather biotechnology and microbiology is as follows.

Pre-requisites:

Fundamentals of Basic Sciences of Higher Secondary Standards.

Course Objectives:

1. To understand the microbiology and their applications to Leather.
2. To understand the application of biotechnology in leather industry.
3. To know the importance of Protein in terms of leather production.
4. Know the applications of enzymes in leather processing.

Course Outcomes:

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UNIT-I MICROSCOPY

Concepts of microscopy – Types of microscope – Light – Bright field, dark field, fluorescent, Phase contrast – Electron Microscopy – X-ray/UV ray microscope – Study and Set up of Compound microscope – Defects and rectifications – Slide preparation, fixing, embedding, sectioning, staining& mounting – Fiber structure and assessment – Orientation and optimal condition of fiber structure – Fiber structural changes takes place during pre-tanning, tanning, post
tanning process – Microscopical Assessment of sole And Various finished leathers -Microstructure of Buff, cow, goat and sheep – Grain pattern of different hides/skins – Collagen study by electron microscope technique.

UNIT -II BACTERIOLOGY

Fundamentals of Bacteriology – Morphological Characteristics of Bacteria – Classification of Bacteria – Staining of Bacteria and its classification – Biochemical Properties of Bacteria -Growth cycle of bacteria and nutrition – Physical conditions required for growth – Effect of environmental factors on bacterial growth – Preparation of various culture media -Sterilization, inoculation, Bacterial count – Control of bacterial growth – Bacterial action on raw hides and skins and in different stages of leather manufacture – And in finished leather -Bacteriological evidence of pollution – Study of Bactericides.

UNIT-III MYCOLOGY & ENTOMOLOGY

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UNIT- IV ENZYMOLOGY

Enzymes- Definition, classification and characterization – Chemical and physical properties of enzymes – Chemistry of enzymes- nature and mechanism – Enzymes in leather processing -Uses of enzymes – Proteolytic enzymes- classification, reactive groups and their functions -Assay, characterization – Enzyme kinetics – Immobilized enzymes – Amylase, lipase, proteaseproduction of microbial enzymes – Fermentation – types – Conditions affecting enzyme activity – Enzyme synthesis – Enzyme preparation, regulation & mechanism.

UNIT- V PROTEIN

Amino Acids: Classification & Structure of Standard amino acids & its properties – Protein: Chemistry – Classification on Fibrous Nature, different levels of protein structure, forces stabilizing protein structure, protein folding, protein modification.

UNIT- VI CARBOHYDRATES & LIPIDS

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Reference Books:

1. Microbiology- Pelczar/ Chan/ Krieg
2. Principles of Biochemistry – Leninger
3. Enzyme Technology in beam house production- Puvanakrishnan&Dhar
4. Fundamental Principles of bacteriology – Salle,A.J
5. Mackie and McCartney’s handbook of bacteriology- MackieT.J
6. Practical Bacteriology- Tanner.F.W.
7. Biochemistry – Voet&Voet – John Wiley & Sons Publication.

SUGGESTED LIST OF STUDENT ACTIVITIES

Note: The following activities or similar activities for assessing CIE (IA) for 5 marks (Any one)

1. Each student should do any one of the following type activity or any other similar activity related to the course and before conduction, get it approved from concerned Teacher and HOD.
2. Each student should conduct different activity and no repeating should occur

1. Prepare the slide for microscopic observation of membrane of onion and submit the step by step procedure in hand written form in 2 to 3 pages.
2. Demonstrate the procedure for differentiation between gram positive & gram negative bacteria and submit the report of complete procedure in hand written form.
3. Collect the information about various types of enzymes used in the leather industry and prepare a detailed report in handwritten form in 2 to 3 pages.
4. Collect the information about the importance of protein & carbohydrates for the existence of life and prepare a detailed report in handwritten form in 2 to 3 pages.

Course Delivery:

The course will be delivered through lectures and presentations.

MODEL QUESTION PAPER (CIE)

1. Demonstrate the fixing in slide preparation.
2. Point outthe importance of electron microscope.
3. Differentiate between gram positive and gram negative bacteria. or Explain the bacteriological evidence in putrefaction.
4. Describe the morphological characteristics of bacteria.or How bacterial growth can be controlled?

MODEL QUESTION PAPER (SEE)

NOTE:

1. Answer any 6 questions from section A & 7 full questions from section B.
2. Each question carries 5 marks in section A.
3. Each question carries 10 marks in section B.

SECTION – A

1. Point out the importance of electron microscope. 5
2. Describe about microscopy. 5
3. Explain about the embedding in slide preparation. 5
4. What is the staining of bacteria? Explain in brief. 5
5. Interpret about the mounting. 5
6. Demonstrate the sectioning in slide preparation. 5
7. Show the orientation of fiber structure in leather with a neat and clean diagram. 5
8. Demonstrate the fixing in slide preparation. 5
9. Discuss the morphological characteristics of bacteria. 5

SECTION – B

1. Draw a neat and clean diagram of compound microscopy & explain the function in brief. 6
2. Write a note on limitations of electron microscopy. 4
1. Draw the microstructure of Buffalo & Cow hides. 6
2. Explain inoculation in brief. 4
1. Draw the microstructure of goat and sheep skins. 6
2. Explain bacterial count. 4
1. Describe various light microscopes. 6
2. How will you examine effect of environmental factors on the growth of bacteria? 4
1. Describe the slide preparation for microscopic observation. 6
2. Draw the cellular structure of Fungi. 5
1. Explain the principal of sterilization. 5
2. How bacterial growth can be controlled? Discuss briefly. 5
1. Discuss about Bactericides? 5
2. Explain the difference between gram positive and gram negative bacteria. 5
1. Explain the bacteriological evidence in putrefaction. 5
2. Outline the effects of mould growth on raw hides/skins and in different stages of leather manufacture. 5
1. Write the different parasitic species that attack the leather. 5
2. What is the effect of mould growth during the transportation of the leather goods? Explain briefly. 5
1. Describe about the parasitic attack on livestock that affects the quality of leather. 5
2. Distinguish between Bacteria & Fungi. 5

For detail syllabus of all other subjects of BE Leather Tech, C15 scheme do visit Leather Tech 3rd Sem syllabus for C15 scheme.

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