{"id":17786,"date":"2021-11-23T07:36:12","date_gmt":"2021-11-23T07:36:12","guid":{"rendered":"https:\/\/www.inspirenignite.com\/up\/departmental-elective-labs-i-kbt751a-genomics-and-proteomic-lab-biotechnology-7th-sem-syllabus-for-aktu-b-tech-2021-22-scheme\/"},"modified":"2021-11-23T07:36:12","modified_gmt":"2021-11-23T07:36:12","slug":"departmental-elective-labs-i-kbt751a-genomics-and-proteomic-lab-biotechnology-7th-sem-syllabus-for-aktu-b-tech-2021-22-scheme","status":"publish","type":"post","link":"https:\/\/www.inspirenignite.com\/up\/departmental-elective-labs-i-kbt751a-genomics-and-proteomic-lab-biotechnology-7th-sem-syllabus-for-aktu-b-tech-2021-22-scheme\/","title":{"rendered":"(Departmental Elective Labs-I) KBT751A: Genomics and Proteomic Lab Biotechnology 7th Sem Syllabus for AKTU B.Tech 2021-22 Scheme"},"content":{"rendered":"<p align=\"justify\">Genomics and Proteomic Lab detail syllabus for Biotechnology (Biotechnology), 2021-22 scheme is taken from <a class=\"rank-math-link\" href=\"https:\/\/aktu.ac.in\/\" style=\"color: inherit\" rel=\"nofollow noopener\" target=\"_blank\">AKTUs<\/a> official website and presented for the AKTU B.Tech students. For the course code (KBT751A), exam duration, teaching hr\/week, practical hr\/week, total marks, internal marks, theory marks, duration, credits, and other details do visit complete semester subjects post given below.<\/p>\n<p>For the Biotechnology 7th Sem Syllabus for AKTU B.Tech 2021-22 Scheme you can visit <a href=\"..\/biotechnology-7th-sem-syllabus-for-aktu-b-tech-2021-22-scheme\">Biotechnology 7th Sem 2021-22 Scheme<\/a>. For the Departmental Elective Labs-I scheme of Biotechnology 7th Sem 2021-22 regulation do refer to <a href=\"..\/departmental-elective-labs-i-biotechnology-7th-sem-syllabus-for-aktu-b-tech-2021-22-scheme\">Departmental Elective Labs-I Biotechnology 7th Sem scheme<\/a>. The detail syllabus for genomics and proteomic lab is as follows.<\/p>\n<p>  <title>Genomics and Proteomic Lab<\/title><\/p>\n<h4>PreRequisites:<\/h4>\n<p>  Molecular Biology &amp; Biochemistry<\/p>\n<h4>Course Objectives:<\/h4>\n<ul>\n<li>To isolate genetic materials from bacterial cells<\/li>\n<li>To determine and characterization of enzyme kinetics<\/li>\n<\/ul>\n<h4>Course Outcomes:<\/h4>\n<p>  After completion of this course successfully, the students will be able to:<\/p>\n<ul>\n<li>Express and purify recombinant protein in bacterial system<\/li>\n<li>Characterized purified protein through enzymatic activity and kinetics<\/li>\n<li>Work with publically available genome sequencing data<\/li>\n<li>Perform genomic DNA and RNA isolation from bacteria and independently setup qRT-PCR<\/li>\n<\/ul>\n<h4>Reference Books:<\/h4>\n<ol>\n<li>Application of DNA microarrays in Biology 2005<\/li>\n<li>Functional Proteomics 2005<\/li>\n<li>Proteomic to study genes and genomes 2000<\/li>\n<\/ol>\n<h4>List of Experiments:<\/h4>\n<ol>\n<li>Expression of heterologous protein in bacterial system.<\/li>\n<li>Detection of expressed protein.<\/li>\n<li>Purification of recombinant protein.<\/li>\n<li>Characterization of purified protein using enzymatic activity.<\/li>\n<li>Determination of kinetic parameters.<\/li>\n<li>Browsing through various publically available genome databases, retrieving and working with genome data. KEGG genomes, retrieval of genes, proteins and intergenic regions.<\/li>\n<li>Genomic DNA isolation from bacterial strains and estimation of DNA concentration using Nanodrop, agarose gel electrophoresis.<\/li>\n<li>RNA isolation from Synechocystis and quality check (RIN number) using Bioanalyser chip, cDNA synthesis and qRT-PCR analysis.<\/li>\n<li>Demonstration of Agilent&#8217;s microarray scanner and DNA microarray protocol.<\/li>\n<li>Raw data processing, scatter plots, global normalization of data, fold changes and finding differentially expressed genes.<\/li>\n<\/ol>\n<p align=\"justify\">For the syllabus of all the subjects of B.Tech Biotechnology 7th Sem, 2021-22 scheme do visit <a class=\"rank-math-link\" href=\"..\/category\/biotechnology+7th-sem\">Biotechnology 7th Sem syllabus subjects<\/a>.<\/p>\n<p id=\"istudy\" style=\"text-align:center\">For the complete syllabus, results, class timetable, and many other features kindly download the <a class=\"rank-math-link\" href=\"https:\/\/play.google.com\/store\/apps\/details?id=ini.istudy\" rel=\"nofollow noopener\" target=\"_blank\">iStudy App<\/a><br \/><b> It is a lightweight, easy to use, no images, and no pdfs platform to make students&#8217;s lives easier.<\/b><br \/><a class=\"rank-math-link\" href=\"https:\/\/play.google.com\/store\/apps\/details?id=ini.istudy&amp;pcampaignid=pcampaignidMKT-Other-global-all-co-prtnr-py-PartBadge-Mar2515-1\" rel=\"nofollow noopener\" target=\"_blank\"><img decoding=\"async\" src=\"https:\/\/play.google.com\/intl\/en_us\/badges\/static\/images\/badges\/en_badge_web_generic.png\" alt=\"Get it on Google Play\" style=\"height:65px\"><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Genomics and Proteomic Lab detail syllabus for Biotechnology (Biotechnology), 2021-22 scheme is taken from AKTUs official website and presented for the AKTU B.Tech students. For the course code (KBT751A), exam [&hellip;]<\/p>\n","protected":false},"author":2300,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[45],"tags":[],"class_list":["post-17786","post","type-post","status-publish","format-standard","hentry","category-biotechnology"],"_links":{"self":[{"href":"https:\/\/www.inspirenignite.com\/up\/wp-json\/wp\/v2\/posts\/17786","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.inspirenignite.com\/up\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.inspirenignite.com\/up\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/up\/wp-json\/wp\/v2\/users\/2300"}],"replies":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/up\/wp-json\/wp\/v2\/comments?post=17786"}],"version-history":[{"count":0,"href":"https:\/\/www.inspirenignite.com\/up\/wp-json\/wp\/v2\/posts\/17786\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.inspirenignite.com\/up\/wp-json\/wp\/v2\/media?parent=17786"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/up\/wp-json\/wp\/v2\/categories?post=17786"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/up\/wp-json\/wp\/v2\/tags?post=17786"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}