Genetic Engineering detail syllabus for Biotechnology (Biotech), 2019-20 scheme is taken from AKTU official website and presented for AKTU students. The course code (RBT-501), and for exam duration, Teaching Hr/Week, Practical Hr/Week, Total Marks, internal marks, theory marks, duration, and credits do visit complete sem subjects post given below.
For all other biotech 5th sem syllabus for b.tech 2019-20 scheme aktu you can visit Biotech 5th Sem syllabus for B.Tech 2019-20 Scheme AKTU Subjects. The detail syllabus for genetic engineering is as follows.
Unit I
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Unit II
Concept of PCR; DNA polymerases; primer designing, linkers, adapters, setting up PCR reactions; Various types of PCR; Applications of PCR in disease diagnostics, forensic sciences and genetic engineering.
Unit III
Gene expression in prokaryotes & eukaryotes, Tissue specific promoter, wound inducible promoters, Strong and regulatable promoters, promoter analysis (EMSA and DNA footprinting), gene expression profiling (real time PCR, SAGE, differential display, Microarray); DNA sequencing methods; Molecular markers: RAPD, RFLP, AFLP, SNP; Site directed mutagenesis, gene silencing techniques.
Unit IV
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Unit V
Basic concepts of cell signaling, Extracellular signal molecule and their receptors, Operation of Signaling molecules over various distances, Cellular response to specific combinations of extracellular signal molecules; Nuclear receptor; Ion channel linked, G-protein mediated receptors, Relay of signal by activated cell surface receptors via intracellular signaling proteins, Intracellular Signaling proteins as molecular switches.
Text and References:
- T.A Brown (2006). Gene cloning and DNA analysis, WILEY-BLACKWELL
- Genetic Engineering by Dr Smita Rastogi & Dr Neelak Pathak, Oxford University Press
- S.B Primrose (2001). Molecular biotechnology. Panima Publishing corporation, 2nd edition
- Molecular Cloning, A laboratory Manual. Sambrook, J., Fritsch, E.F., Mariatis.3rd edition
- Genetic Engineering, Priciples & Practice by Sandhya Mitra, McGraw Hill Education.
For detail syllabus of all other subjects of B.Tech Biotech, 2019-20 scheme do visit Biotech 5th Sem syllabus for 2019-20 scheme.
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