Experimental Biotechnology (Nptel) Biotech 8th Sem Syllabus for AKTU B.Tech 2019-20 Scheme (Departmental Elective-V)

Experimental Biotechnology (Nptel) detail syllabus for Biotechnology (Biotech), 2019-20 scheme is taken from AKTU official website and presented for AKTU students. The course code (RBT081), and for exam duration, Teaching Hr/Week, Practical Hr/Week, Total Marks, internal marks, theory marks, and credits do visit complete sem subjects post given below.

For all other biotech 8th sem syllabus for b.tech 2019-20 scheme aktu you can visit Biotech 8th Sem syllabus for B.Tech 2019-20 Scheme AKTU Subjects. For all other Departmental Elective-V subjects do refer to Departmental Elective-V. The detail syllabus for experimental biotechnology (nptel) is as follows.

Course Outline:

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Course Plan:

1. Introduction Introduction, Concept of buffers.
2. Spectroscopic experiments: Spectroscopic techniques: UV-Visible spectroscopy, Fluorescence spectroscopy, CD spectroscopy, IR spectroscopy. Protein estimation 1, 2, DNA estimation, unfolding, Protein unfolding, CD, IR
3. electrophoretic experiments: Electrophoresis; Principle, performing electrophoresis techniques, application of electrophoresis in analyzing macromolecules.
4. Chromatographic experiments: Chromatographic techniques; Principles, Column chromatography, HPLC
5. Immunological experiments: Antibody generation and purification, Immuno-assays to detect and quantify antigens.
6. microscopic experiments: Light Microscopy; Fluorescence microscopy, Atomic forcemicroscope, Electron microscope, Scanning electronmicroscopy, Transmission Electron microsope.
7. Molecular cloning Preparation and transformation of competent cells; Small scale isolation of recombinant plasmid, Analysis of the recombinant plasmid using Restriction Endonucleases.

Reference Books:

1. 1. A. J. Ninfa and D. P. Ballou, Fundamental Laboratory Approaches for Biochemistry and Biotechnology, Wiley; 2nd Edition,1998.
   2. J. Sambrook and D. W. Russell, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, 3rd Edition, 2001.
   3. Live Cell Imaging: A Laboratory Manual R. D. Goldman, J. R. Swedlow and D. L. Spector Cold Spring.Harbor Laboratory Press; 2nd edition, 2009
   4. Basic Methods in Microscopy, Protocols and concepts from cells: A Laboratory Manual, D. L. Spector & R. D. Goldman (Editors.), Cold Spring Harbor Laboratory Press, 2006
   5. A. Manz, N. Pamme and D. Iossifidis, Bioanalytical Chemistry, World Scientific Publishing Company, 2004
   6. R.J. Simpson, Proteins and Proteomics: A Laboratory Manual, CSHL press, 2003

http://nptel.ac.in

For detail syllabus of all other subjects of B.Tech Biotech, 2019-20 regulation do visit Biotech 8th Sem syllabus for 2019-20 Regulation.

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