Industrial Biotechnology Lab detail syllabus for Biotechnology (Biotechnology), 2021-22 scheme is taken from AKTUs official website and presented for the AKTU B.Tech students. For the course code (KBT751D), exam duration, teaching hr/week, practical hr/week, total marks, internal marks, theory marks, duration, credits, and other details do visit complete semester subjects post given below.
For the Biotechnology 7th Sem Syllabus for AKTU B.Tech 2021-22 Scheme you can visit Biotechnology 7th Sem 2021-22 Scheme. For the Departmental Elective Labs-I scheme of Biotechnology 7th Sem 2021-22 regulation do refer to Departmental Elective Labs-I Biotechnology 7th Sem scheme. The detail syllabus for industrial biotechnology lab is as follows.
PreRequisites:
Knowledge of Microbiology, Biochemistry
Course Objectives:
- To produce various bioproducts from cells and tissues.
- To isolate the industrially important microorganisms.
- To study techniques for isolation and purification of bioproducts.
- To provide the knowledge about determining the various important characteristic of industrially important enzymes.
Course Outcomes:
After successful completion of the course the students will be able to:
- Demonstrate the production of bioproducts like amylase and citric acid and ethanol.
- Isolate the amylolytic microorganism.
- Purify the industrially important enzymes and proteins.
- Can perform the enzymatic assay.
Reference Books:
- Glazer AN, Nikaido H : Microbial Biotechnology: Fundamentals of Applied Microbiology Eisenthal, R. & Danson N.J. (Eds) Enzyme Assays: ‘APractical Approach’, IRI Press, Oxford, UK 1992
- Industrial Biotechnology: Products and Processes 2016
- Laboratory Manual In Industrial BiotechnologyBy P. Chellapandi 2007
- Practical Manual on Fermentation Technology by S. Kulandaivelu, S. Janarthanan 2012
List of Experiments:
- Production and partial purification of Amylase in shake flask culture.
- Isolation of amylolytic microorganisms.
- Production of Citric acid using Aspergillus species.
- Protein precipitation and membrane based separation using dialysis.
- Purification of Enzyme by ammonium sulphate fractionation.
- Comparative studies of ethanol production using different substrates.
- Determination of cellulolytic activity by DNS method.
- Enzyme assay; activity and specific activity determination of amylase.
For the syllabus of all the subjects of B.Tech Biotechnology 7th Sem, 2021-22 scheme do visit Biotechnology 7th Sem syllabus subjects.
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