Animal Biotechnology detail syllabus for Biotechnology (Biotech), 2019-20 scheme is taken from AKTU official website and presented for AKTU students. The course code (RBT061), and for exam duration, Teaching Hr/Week, Practical Hr/Week, Total Marks, internal marks, theory marks, and credits do visit complete sem subjects post given below.
For all other biotech 6th sem syllabus for b.tech 2019-20 scheme aktu you can visit Biotech 6th Sem syllabus for B.Tech 2019-20 Scheme AKTU Subjects. For all other Departmental Elective-II subjects do refer to Departmental Elective-II. The detail syllabus for animal biotechnology is as follows.
Unit I
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Unit II
Different tissue culture techniques; Types of primary culture; Chicken embryo fibroblast culture; Chicken liver and kidney culture; Secondary culture; Trypsinization; Cell separation; Continuous cell lines; Suspension culture; Organ culture etc.; Behavior of cells in culture conditions: division, growth pattern, metabolism of estimation of cell number; Development of cell lines; Characterization and maintenance of cell lines, stem cells; Cryopreservation; Common cell culture contaminants.
Unit III
Cell cloning and selection; Transfection and transformation of cells; Commercial scale production of animal cells, stem cells and their application; Application of animal cell culture for in vitro testing of drugs; Testing of toxicity of environmental pollutants in cell culture; Application of cell culture technology in production of human and animal viral vaccines and pharmaceutical proteins.
Unit IV
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Unit V
Transgenic animal production; Methods of transgene delivery; Integration of foreign genes and their validation; Gene targeting; Methods and strategies; Improving transgene integration efficiency; Cell lineages and developmental control genes in drosophila and mice; Differentiation of germ layers; Cellular polarity; Stem cell differentiation; Blood cell formation; Fibroblasts and their differentiation; Differentiation of cancerous cells and role of protooncogenes;
Text Books:
- B. Hafez and E.S.E Hafez, Reproduction in farm animals, 7th Edition, Wiley Blackwell, 2000
- G.E. Seidel, Jr. and S.M. Seidel, Training manual for embryo transfer in cattle (FAO Animal Production and Health Paper-77), 1st Edition, W.D. Hoard and sons FAO, 1991
- I. Gordon, Laboratory production of cattle embryos, 2nd edition, CAB International, 2003.
- Louis-Marie Houdebine, Transgenic Animals: Generation and Use 5th Edition, CRC Press, 1997.
For detail syllabus of all other subjects of B.Tech Biotech, 2019-20 regulation do visit Biotech 6th Sem syllabus for 2019-20 Regulation.
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