Enzyme Engineering detailed syllabus scheme for Biotechnology (BT), 2018 regulation has been taken from the University of Mumbai official website and presented for the Bachelor of Engineering students. For Course Code, Course Title, Test 1, Test 2, Avg, End Sem Exam, Team Work, Practical, Oral, Total, and other information, do visit full semester subjects post given below.
For all other Mumbai University Biotechnology 6th Sem Syllabus 2018 Pattern, do visit BT 6th Sem 2018 Pattern Scheme. The detailed syllabus scheme for enzyme engineering is as follows.
Enzyme Engineering Syllabus for Biotechnology TE 6th Sem 2018 Pattern Mumbai University
Prerequisites:
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Course Objectives:
The objectives of this course are to:
- Give insight into the functioning of Enzyme molecules (Biological Catalyst), their constructions, Structure, interactions with other cellular molecules, and the process of catalysis.
- Students will learn to use such molecules for making of difficult biomolecules.
- They will also be able to understand Industrial uses and applications of Enzymes.
Course Outcomes:
By learning this course the students will be able to:
- Understand how Enzymes are created as functional bio-catalysts, analysed with respect to their effciencies, their lability, and ways to make them durable.
- They also will be familiar with the problems they could encounter and how to trouble shoot them.
- They will be able to monitor both in-vitro and in-vivo activity.
Module 1
Enzymes as Biological Catalysts, Chemical nature & Structure of Enzymes, Properties of enzymes, Enzyme Units, Nomenclature & Classification, Catalytic and Allosteric site, Models of Enzyme-substrate interactions, Activation Energy and catalysis, Effect of pH, Temperature and Salts on Enzyme activity 08
Module 2
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Module 3
Enzyme Assay: Kinetic determination of catalytic activity, Coupled kinetic assay, Radioimmunoassay. Instrumental techniques for Enzymatic Analysis: Manometry, Spectrophotometry, Spectrofluorimetry, Electrochemical methods (Potentiometric & Conductometry) Enthalpimetry, Radiochemical methods, Dry reagent techniques. 06
Module 4
Working with Enzyme: Isolation & Extraction of Enzymes from various sources and locations in cell. Purification of Enzymes: Preliminary purification, further purification using chromatography & electrophoresis Criteria of Purity: Specific Activity, Electrophoresis, And 08 Ultracentrifugation. Immobilization of Enzyme for repeat use.
Module 5
Solid-liquid reactors for enzymes: Types of heterogeneous reactors and its design criteria (CSTR, PFR, PBR, FBR etc), Operational problems, Decline and loss of enzyme efficiency, Remedies. 07
Module 6
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Assessment
Internal
- Assessment consists of two tests which should be conducted at proper intervals.
End Semester theory examination
- Question paper will comprise of 6 questions each carrying 20 questions.
- Total 4 questions need to be solved
- Question no.1 will be compulsory based on entire syllabus wherein sub questions can be asked.
- Remaining questions will be randomly selected from all the modules
- Weightage of marks should be proportional to number of hours assigned to each module
Reference Books:
- Enzymes-Palmer; T, (Affliated East West Press Pvt. Ltd), 2004
- Biochemistry- Stryer, Berg, 6thEdition, (W.H. Freeman and Co), 2007
- Biochemistry-Metzler; DE, 2nd Edn., (Academic press) 2001
- Lehninger Principles of biochemistry-Nesson, Cox, 4th Edn., (W.H. Freeman and Co), 2005
- Biochemistry Voet & Voet; J, 3rd Edn. (John Wiley and sons Inc), 2004
- Outlines of Biochemistry-Conn; E,Stumpf, 5thEdn.(Tata-McGraw Hill), 1988
- Enzyme Biotechnology- Tripathi; G, (ABD Publishers), 2003
- Enzyme Technology, M.F. Chaplin and C. Bucke. Cambridge University Press
- Industrial Enzymes & their applications, H. Uhlig, (John Wiley and Sons Inc)
For detail syllabus of all other subjects of Biotechnology (BT) 6th Sem 2018 regulation, visit BT 6th Sem Subjects syllabus for 2018 regulation.