Cell & Tissue Culture detailed syllabus scheme for Biotechnology (BT), 2018 regulation has been taken from the University of Mumbai official website and presented for the Bachelor of Engineering students. For Course Code, Course Title, Test 1, Test 2, Avg, End Sem Exam, Team Work, Practical, Oral, Total, and other information, do visit full semester subjects post given below.
For all other Mumbai University Biotechnology 6th Sem Syllabus 2018 Pattern, do visit BT 6th Sem 2018 Pattern Scheme. The detailed syllabus scheme for cell & tissue culture is as follows.
Cell & Tissue Culture Syllabus for Biotechnology TE 6th Sem 2018 Pattern Mumbai University
Prerequisites:
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Course Objectives:
- To examine and analyse practical and theoretical principles of cell culture
- To explain the conditions under which cells can be cultured outside the body.
- To explain the advantages and limitations of cell culture in biomedical research and applications.
Course Outcomes:
By learning this course the students will be able to:
- Plan experiments using cultured cells.
- Carry out cell culture, and associated laboratory techniques.
- Carry out the most common analysis techniques associated with cell culture.
- Perform adequate statistical processing of data generated by cell culture.
- Present and analyse literature which covers cell culture.
Module 1
Plant tissue culture Introduction: Internal organization of plant, Plant Tissue Culture Media, Plant growth hormones, Concept of Totipotency, Study of various types of Organ Culture, Organogenesis, Mi cropropagati on 08
Module 2
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Module 3
Transformation of Plants: Agrobacterium mediated Gene transfer, Chemical Methods, Virus Mediated Gene Transfer, Transgenic Plants, Applications of Plant Biotechnology for Production of Quality Oil, Industrial Enzymes and Plantibodies 06
Module 4
Introduction to Animal Cell Culture: Advantages and limitations of Animal Tissue Culture, Laboratory Design & Layout of ATC laboratory, Equipments and Materials of a Tissue Culture Laboratory, Media Preparation and Sterilization techniques, 08
Module 5
Primary and secondary cell Culture: Establishment and maintenance of primary cell cultures of adherent and non-adherent cell lines, Establishment and maintenance of seconary and continuous cell cultures. 05
Module 6
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Module 7
Application of cell culture: Vaccine production, Antibody Engineering and large scale production of pharmaceutical products, Production of cytokines and therapeutic recombinant glycoproteins. 05
Assessment
Internal
- Assessment consists of two tests which should be conducted at proper intervals.
End Semester theory examination
- Question paper will comprise of 6 questions each carrying 20 questions.
- Total 4 questions need to be solved
- Question no.1 will be compulsory based on entire syllabus wherein sub questions can be asked.
- Remaining questions will be randomly selected from all the modules
- Weightage of marks should be proportional to number of hours assigned to each module
Reference Books:
- Plant Tissue Culture: Theory and Practice :Theory and Practice By S.S. Bhojwani, M.K. Razdan; Elsevier Publishers
- Plant Tissue Culture by Kalyan Kumar De; Published by New Central Book Agency, 1997
- Plant Tissue and Cell Culture; Volume11 of Botanical monographs, by Herbert Edward Street; Publisher: University of California Press, 1973
- Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications By R. Ian Freshney; Wiley Publishers
- Animal Cell Culture ( Introduction to Biotechniques): Sara j.Morgan, David C. Darling; Published by BIOS Scientific Publishers Ltd., 1993
- Principles and Practice of Animal Tissue Culture – Sudha Gangal.
- Basic Cell Culture Vol. 290 Protocols by Cheryl D Helgason, Cindy L Miller. Humanan Press.2005.
- Basic Cell Culture 2nd Edition by JM Davis Oxford University Press.2002.
- Biotechnology by B. D. Singh: Expanding horizons. Kalyani Publishing. 2008.
- Animal Cell Culture & Technology by M. Butler, Published by Taylor & Francis publishers.
For detail syllabus of all other subjects of Biotechnology (BT) 6th Sem 2018 regulation, visit BT 6th Sem Subjects syllabus for 2018 regulation.