BTC404: Analytical Methods in Biotechnology Syllabus for BT 4th Sem 2017 Pattern Mumbai University

Analytical Methods in Biotechnology detailed syllabus scheme for Biotechnology (BT), 2017 regulation has been taken from the University of Mumbai official website and presented for the Bachelor of Engineering students. For Course Code, Course Title, Test 1, Test 2, Avg, End Sem Exam, Team Work, Practical, Oral, Total, and other information, do visit full semester subjects post given below.

For all other Mumbai University Biotechnology 4th Sem Syllabus 2017 Pattern, do visit BT 4th Sem 2017 Pattern Scheme. The detailed syllabus scheme for analytical methods in biotechnology is as follows.

Analytical Methods in Biotechnology Syllabus for Biotechnology SE 4th Sem 2017 Pattern Mumbai University

Prerequisites:

For the complete Syllabus, results, class timetable, and many other features kindly download the iStudy App
It is a lightweight, easy to use, no images, and no pdf platform to make students’s lives easier.
.

Course Objectives:

• To study the various analytical techniques used in Biotechnology.

Course Outcomes:

• The students will be capable of handling different instruments in the laboratory.
• They would be able to compare different separation techniques and use them effectively in research work

Module 1

Centrifugation:

1. General principle- sedimentation velocity, sedimentation equilibrium
2. Types of centrifuges, preparative and analytical centrifugation, differential centrifugation, density gradient methods
3. Applications 08

Module 2

For the complete Syllabus, results, class timetable, and many other features kindly download the iStudy App
It is a lightweight, easy to use, no images, and no pdf platform to make students’s lives easier.
.

Module 3

Electro kinetic methods of separation:

1. Electrophoresis: General principle and application, factors affecting electrophoresis – voltage, current, resistance, buffer, composition, concentration, pH.
2. Agarose Gel electrophoresis
3. SDS-PAGE – Native and denaturing gels, gradient gels, discontinuous buffer system
4. Two dimensional gel electrophoresis
5. Isoelectric focusing
6. Capillary electrophoresis 09
7. Immuno electrophoresis

Module 4

Spectroscopy:

1. Spectroscopic Techniques; Beers Lamberts law, molar and extinction coefficient, limitations of Beers Lamberts law
2. Visible and UV Spectrophotometry; Principles, Instrumentation and applications 08

Module 5

Radio isotopic techniques:

1. Use of radioisotopes in life sciences, radioactive labelling, principle and application of tracer techniques
2. Detection and measurement of radioactivity using ionization chamber, proportional chamber, Geiger-Muller and Scintillation counters, Autoradiography
3. Applications 08

Assessment

For the complete Syllabus, results, class timetable, and many other features kindly download the iStudy App
It is a lightweight, easy to use, no images, and no pdf platform to make students’s lives easier.
.

Reference Books:

1. Wilson K and Goulding K.H., A biologists guide to Principles and Techniques of Practical Biochemistry.
2. Willard and Merrit, Instrumental Methods and Analysis
3. Ewing GW, Instrumental Methods of Chemical analysis.
4. Robert. M. Silverstein et al, Spectrometric identification of Organic Compounds, 7th Edition, 1981.
5. Vogels, Text Book of Quantitative Chemical Analysis, 6th Edition, 2004.
6. John A. Adamovic, Chromatographic Analysis of Pharmaceuticals, 2nd Edition.
7. Raymond P. W. Scott, Techniques and Practice of Chromatography -Vol 70
8. Sethi P.D, Dilip Charegaonkar, Chromatography -2nd Edition.
9. Niessen W. M. A., Van Der Greef J, Liquid Chromatography- Mass Spectrometry, Vol 58
10. Kalsi. P.K, Spectroscopy of Organic Compounds.
11. Hanes, Gel Electrophoresis of Proteins- A Practical Approach,
12. Hamilton R. J. and Sewell P. A, Introduction to High Performance Liquid Chromatography
13. Gordon M. Message, Practical aspects of Gas Chromatography and Mass Spectrometry, John Wiley and Sons, New York. 1984
14. Chapman J.M and G. Ayrey, The use of radioactive isotopes in the life sciences, George Allen and Unwin Ltd., London.
15. Biophysical chemistry by Upadhyay, Upadhyay and Nath, Himalaya publication house.

For detail syllabus of all other subjects of Biotechnology (BT) 4th Sem 2017 regulation, visit BT 4th Sem Subjects syllabus for 2017 regulation.