Microbiology detailed syllabus scheme for Biotechnology (BT), 2017 regulation has been taken from the University of Mumbai official website and presented for the Bachelor of Engineering students. For Course Code, Course Title, Test 1, Test 2, Avg, End Sem Exam, Team Work, Practical, Oral, Total, and other information, do visit full semester subjects post given below.
For all other Mumbai University Biotechnology 3rd Sem Syllabus 2017 Pattern, do visit BT 3rd Sem 2017 Pattern Scheme. The detailed syllabus scheme for microbiology is as follows.
Microbiology Syllabus for Biotechnology SE 3rd Sem 2017 Pattern Mumbai University
Prerequisites:
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Course Objectives:
- The course aims to develop skills of the Students in the area of Microbiology particularly to identify microbes, their structure, their metabolism and their industrial applications.
- They will study various sterilization techniques and their effects.
- This will be a prerequisite for all courses offered in Bioprocess Technology
Course Outcomes:
- Students will be able to carry out various microbiological techniques like staining and isolation very well.
- They would be able to identify microbes.
- They would have detailed knowledge of various sterilization techniques, which would be useful for other courses.
Module 1
History and Scope of Industrial Microbiology:
- Introduction: Discovery of Microbial world
- The experiments of Pasteur; The discovery of Anaerobic Life
- Physiological significance of Fermentation; Pasteur and Fermentation
- The Era of discovery of Antibiotics; Growth of Industrial fermentation 03
Module 2
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Module 3
Microbial Nutrition:
- Nutritional requirements of microorganisms
- Different types of media- Synthetic media, complex media Selective media, differential media, enrichment media. 05
Module 4
Microbial Growth:
- Introduction: phases of growth
- Growth curve
- Kinetics of growth
- Measurement of growth
- Continuous &batch culture
- Synchrony
- Chemostat & turbidostat
- Effects of solutes, temperature, ion concentration, oxygen, hydrostatic pressure, heavy metal ions, and UV light on microbial growth 05
Module 5
Microbiological Techniques:
- Sterilization and disinfection techniques,
- Principles and methods of sterilization.
- Physical methods – autoclave, hot-air oven, pressure cooker, laminar airflow, filter sterilization.
- Radiation methods – UV rays, gamma rays, ultrasonic methods.
- Chemical methods – Use of alcohols, aldehydes, fumigants, phenols, halogens and hypochlorites. Phenol coefficient.
- Isolation of pure culture techniques – Enrichment culturing, dilution plating, streak- plate, spread-plate and micromanipulator.
- Preservation of microbial cultures – sub culturing, overlaying cultures with mineral oils, lyophilization, sand cultures, storage at low temperature. 09
Module 6
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Module 7
Water & Soil Microbiology:
- Microbiological analysis of water purity-sanitary tests for coliforms (presumptive test, confirmed test, competed test), MPN test, defined substrate test, IMVIC test.
- Soil microbiology- soil as a habitat for microorganisms, physico-chemical properties of soil, microbial community in soil, role of microorganisms in organic matter decomposition. 07
Assessment
Internal:
- Assessment consists of average of two tests which should be conducted at proper interval.
End Semester theory examination
- Question paper will comprise of 6 questions, each carrying 20 marks.
- Total 4 questions need to be solved.
- Question No.1 will be compulsory and based on entire syllabus wherein sub questions can be asked.
- Remaining questions will be randomly selected from all the modules. Weightage of marks should be proportional to number of hours assigned to each Module.
Reference Books:
- Textbook of Microbiology ;R. Ananthnarayan, C. K. J. Panicker, Orient Longman 6th Edition (2003)
- General Microbiology, R.Y. Stanier, J.L. Ingraham, M.L.Wheelis and P.R. Painter, Macmillian
- Microbiology VI Edition, M.J. Pelczar, E.C.S. Chan and N.R. Kreig, Tata McGraw Hill Microbiology by Prescott
- Microbiology: An Introduction (9th Ed) by Tortora GJ, Funke BR, and Case CL, Pearson Education, 2008.
- Industrial Microbiology, Casida, New Age International
- Industrial Microbiology, Prescott and Dunn, C.B.S. Publishers Principles of Microbiology, R.M. Atlas, WMC. Brown Publisher.
- Microbiology – Fundamentals and Application, 6th Ed. – Purohit, S.S. (Agrobios)
- Textbook of Microbiology, P.Charkborthy
- General Microbiology Vol. II by Powar and Daginawala Himalaya Publ. House 8th edition (2004)
For detail syllabus of all other subjects of Biotechnology (BT) 3rd Sem 2017 regulation, visit BT 3rd Sem Subjects syllabus for 2017 regulation.