325324: Applied Microbiology in Medical Laboratory Technology Syllabus for Medical Laboratory Technology 5th Sem K Scheme MSBTE PDF

Applied Microbiology in Medical Laboratory Technology detailed Syllabus for Medical Laboratory Technology (ML), K scheme PDF has been taken from the MSBTE official website and presented for the diploma students. For Subject Code, Subject Name, Lectures, Tutorial, Practical/Drawing, Credits, Theory (Max & Min) Marks, Practical (Max & Min) Marks, Total Marks, and other information, do visit full semester subjects post given below.

For all other MSBTE Medical Laboratory Technology 5th Sem K Scheme Syllabus PDF, do visit MSBTE Medical Laboratory Technology 5th Sem K Scheme Syllabus PDF Subjects. The detailed Syllabus for applied microbiology in medical laboratory technology is as follows.

Rationale

The curriculum is designed to equip students with essential knowledge and practical skills in microbiology that are crucial for diagnosing and managing infectious diseases. It combines theoretical concepts with hands-on laboratory experiences, encouraging critical thinking and analytical skills necessary for the healthcare industry. This approach ensures that graduates are well-prepared to meet the challenges of modern medical laboratory practices.

Course Outcomes:

Students will be able to achieve & demonstrate the following COs on completion of course based learning

1. Identify and explain fundamental concepts of microbiology
2. Assess the role of microorganisms in human health and disease.
3. Demonstrate laboratory techniques for microbial analysis.
4. Analyze and interpret laboratory results for clinical applications.
5. Investigate current trends and advancements in microbiology.

Unit I

Introduction to Microbiology 1.1 Definition and scope of microbiology 1.2 Structure of bacteria, viruses, fungi, protozoa 1.3 Metabolism (aerobic vs. anaerobic) 1.4 Growth phases of microorganisms 1.5 Classification methods

Suggested Learning Pedagogie
Lectures Interactive discussions Concept mapping Video presentations Group activities

Unit II

Microbial Pathogenesis 2.1 Overview of pathogenic microorganisms 2.2 Mechanisms of disease causation 2.3 Host defenses (innate and adaptive immunity) 2.4 Role of virulence factors 2.5 Epidemiology of infectious diseases

Suggested Learning Pedagogie
Case studies Group discussions Role Play Presentations Collaborative learning

Unit III

Laboratory Techniques in Microbiology 3.1 Laboratory safety protocols 3.2 Culturing techniques for bacteria and fungi 3.3 Staining techniques (Gram stain, acidfast stain) 3.4 Antimicrobial susceptibility testing (disk diffusion, MIC) 3.5 Quality control in microbiological testing

Suggested Learning Pedagogie
Hands-on lab sessions Demonstrations Presentations Collaborative learning Site/Industry Visit

Unit IV

Clinical Microbiology and Interpretation 4.1 Interpreting culture and sensitivity results 4.2 Correlating lab findings with clinical conditions 4.3 Understanding antimicrobial resistance mechanisms 4.4 Effective reporting techniques 4.5 Ethical considerations in microbiology

Suggested Learning Pedagogie
Case analyses Presentations Site/Industry Visit Collaborative learning Flipped Classroom

Unit V

Advancements in Microbiology 5.1 Overview of emerging infectious diseases 5.2 Innovations in diagnostic technologies (PCR, next-gen sequencing) 5.3 Role of biotechnology in microbiology 5.4 Public health measures for infection control 5.5 Global health challenges

Suggested Learning Pedagogie
Site/Industry Visit Flipped Classroom Presentations Video Demonstrations Collaborative learning

List of Experiments:

1. Prepare microbial growth media*
2. Isolation of Bacteria from Clinical Samples*
3. Gram Staining of Bacterial Samples*
4. Perform Acid-Fast Staining*
5. Perform motility testing of bacteria*
6. Perform Antibiotic Susceptibility Testing (Disk Diffusion)*
7. Perform Serial Dilution and Viable Count*
8. Perform Biofilm Formation Assessment
9. Perform Identification of Yeast and Fungi*
10. PCR Amplification of Microbial DNA
11. Perform Culture of Anaerobic Bacteria
12. Perform Serological Testing for Microbial Antigens*
13. Environmental Sampling for Microbial
14. Molecular Techniques in Microbiology (Gel Electrophoresis)
15. Detection of Pathogens using ELISA
16. Reporting and Interpreting Microbial Laboratory Results

Self Learning

Micro Project

• Design a Microbial Cell Model
• Microbial Growth Simulation Project
• Choose a specific infectious disease and document the pathogen, symptoms, transmission, and treatment strategies.
• Create a poster explaining how virulence factors (toxins, adhesion, immune evasion) enable pathogens to cause disease.
• Develop a concise safety guide for students working in a microbiology lab, focusing on protocols and PPE.
• Create a step-by-step video or presentation demonstrating how to culture bacteria and fungi on different media.
• Interpret given lab reports and propose suitable antibiotic treatment based on the results.
• Create a template for reporting microbiological results effectively, with sections for interpretation and recommendations.
• Choose an emerging infectious disease (e.g., COVID-19) and present its challenges, prevention, and control measures.
• Prepare a project demonstrating the use of PCR or CRISPR technology in diagnostics or treatment.

Assignment

• Write a Essay: Importance of Microbiology in Health Sciences
• Prepare a chart comparing bacteria, viruses, fungi, and protozoa based on their structure, reproduction, and examples.
• Describe the interactions between normal microbiota and the immune system
• Choose an infectious disease outbreak and analyze its spread
• Write an assignment explaining the principle of Gram staining, its procedure, and clinical applications.
• Compare different antimicrobial susceptibility tests (disk diffusion, MIC) and explain their relevance in clinical practice.
• Discuss how antimicrobial resistance affects patient outcomes and strategies for combating it.
• Write about ethical considerations in reporting sensitive lab results, including privacy and public health concerns.
• Write about recent innovations in diagnostic tools, such as PCR, MALDI-TOF, or next-gen sequencing.
• Prepare a report on how public health measures like vaccination and surveillance control infectious diseases.

Laboratory Equipment

1. PCR Machine (Thermocycler) 10
2. Anaerobic Jar and Gas Packs 11
3. Centrifuge 12
4. Swabs and Agar Plates 13
5. Gel Electrophoresis Apparatus 14
6. ELISA Reader 15
7. Computer and Reporting Software 16
8. Light Microscope 3
9. Heating Block/Water Bath 4
10. Microtiter Plates 8
11. Autoclave All
12. Laminar Air Flow Cabinet All
13. Pipettes and Microtubes All

Learning Materials

1. Ananthanarayan & Paniker Textbook of Microbiology Universities Press 978-8173717815
2. Chakraborty P. A Textbook of Microbiology New Central Book Agency 9788173815078
3. Baveja CP Textbook of Microbiology for Nurses Arya Publications 978-9350250161
4. Jawetz, Melnick & Adelberg Medical Microbiology McGraw-Hill Education 978-0071818216
5. D.R. Arora Textbook of Microbiology CBS Publishers 978-8123922624

Learning Websites

1. https://www.youtube.com/watch?v=Mo-VT2OGzt4 Culture Media In hindi | Culture Media Microbiology | Culture Media Classification | Use of Media
2. https://www.youtube.com/watch? v=CMfEZ4b-xY8 Culture media Microbiology || Classification of Culture media
3. https://www.youtube.com/watch? v=2jcrReCqQpY Next Generation Sequencing Method
4. https://www.youtube.com/watch? v=nI5AWEIvHJg Polymerase Chain Reaction / PCR in Microbiology
5. https://www.youtube.com/watch? v=85KBu1RLRxQ Types of staining || Comman Staning techniques
6. https://www.youtube.com/watch? v=lPuSuuAbHMg Microbiology lectures |Pathogenesis of bacterial infections|Bacteriology
7. https://www.youtube.com/watch? v=ZcL67RjIGI8 Laboratory Methods for Identification of bacteria explain in Hindi
8. https://www.youtube.com/watch? v=4H0RC_5HUDU What is Microbiology With Full Information? – [Hindi]
9. https://www.youtube.com/watch? v=gJgWRSOH8_g Pathogen Kya Hai | Pathogen Kya Hota Hai | Pathogens in Hindi
10. https://www.youtube.com/watch? v=vfq9vdgqsII Introduction of Microbiology | Branches of Microbiology | Microbiology Lecture in hindi

For detail Syllabus of all other subjects of Medical Laboratory Technology, K scheme do visit Medical Laboratory Technology 5th Sem Syllabus for K scheme.

For all Medical Laboratory Technology results, visit MSBTE Medical Laboratory Technology all semester results direct links.