Advanced Biochemistry detailed Syllabus for Medical Laboratory Technology (ML), K scheme PDF has been taken from the MSBTE official website and presented for the diploma students. For Subject Code, Subject Name, Lectures, Tutorial, Practical/Drawing, Credits, Theory (Max & Min) Marks, Practical (Max & Min) Marks, Total Marks, and other information, do visit full semester subjects post given below.
For all other MSBTE Medical Laboratory Technology 5th Sem K Scheme Syllabus PDF, do visit MSBTE Medical Laboratory Technology 5th Sem K Scheme Syllabus PDF Subjects. The detailed Syllabus for advanced biochemistry is as follows.
Rationale
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Course Outcomes:
Students will be able to achieve & demonstrate the following COs on completion of course based learning
- Interpret the Molecular process in a cell from given sample
- Analyze enzyme in given sample of body fluid.
- Correlate observed biochemical pathway with metabolic disorder.
- Interpret biochemical process of Cancer cell in given sample
- Diagnose the given sample using advanced biochemistry techniques.
Unit I
Molecular Genetics and Biochemistry 1.1 DNA Replication. 1.2 RNA Transcription. 1.3 Translation process. 1.4 Mutation and type of Mutation 1.5 Application of Molecular Genetics in disease diagnosis.
Suggested Learning Pedagogie
Model Demonstration Video Demonstrations Flipped Classroom Hands-on Site/Industry Visit
Unit II
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Unit III
Metabolic disorders and biochemical pathway 3.1 Different biochemical Pathways of Inborn error of Metabolism. 3.2 Metabolic pathway in diabetes mellitus, hyperlipidemia 3.3 Integration of metabolic pathways 3.4 Metabolic process in Starvation 3.5 Diagnostic test for metabolic disorder and clinical interpretation
Suggested Learning Pedagogie
Video Demonstrations Model Demonstration Collaborative learning Flipped Classroom Lecture Using Chalk- Board
Unit IV
Biochemical basis of disease 4.1 Characteristic of cancer cells. 4.2 Carcinogenesis and carcinogens 4.3 Proto-Oncogene and Oncogenes 4.4 Tumor Suppressor gene 4.5 Diagnostic tumor marker
Suggested Learning Pedagogie
Video Demonstrations Flipped Classroom Lecture Using ChalkBoard Site/Industry Visit Collaborative learning
Unit V
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List of Experiments:
- *Isolation of DNA from liver cell
- *Identification of DNA by Diphenylamine reagent.
- Estimation of DNA Calorimetric method.
- Isolation of RNA by Yeast Cell.
- *Detection of RNA from Trizol reagent.
- Separation of RNA from protein by Phenol extraction method
- Estimation of RNA by Orcinol method.
- *Measurment of alkaline phosphatase in serum
- *Measurment of Acid phosphatase enzyme activity
- *Measurment of SGPT,SGOT activity
- *Measurement amylase enzyme activity
- *Isolation of amylase enzyme form saliva
- *Measurment of Creatine kinase activity
- *Separation of amino acid by TLC.
- *Separation of Drugs by Thin Layer Chromatography
Self Learning
Micro Project
- Analyze DNA from various biological sources (e.g., blood, saliva, plant tissues).
- Extract RNA from different biological sources for molecular diagnostic purposes.
- Perform liver function tests (LFTs).
- Collect and analyze blood/urine samples for amylase levels.
- Collect 10 patient samples and analyze clinical markers (e.g., ALT, AST, ALP, bilirubin)
- Case study on role of amylase in digestion and its deficiency-related diseases (e.g., pancreatitis, cystic fibrosis).
- Compare normal and deficient levels of amylase.
- Identification of specific markers for different liver diseases like hepatitis, cirrhosis, and fatty liver.
Assignment
- Diagrammatic representation of TLC on paper sheet.
- Diagrammatic representation of HPLC on paper sheet
- Diagrammatic representation of enzyme-inhibitor complex Formation on paper sheet.
- Prepare presentation on integration of metabolic pathway.
- Prepare presentation on starvation
- Prepare chart for enzyme marker.
- Prepare chart for tumor Marker.
- Prepare model of Electrophoresis.
- Prepare chart for metabolic disorder.
- Prepare model of DNA
- Prepare chart enzyme inhibition.
Laboratory Equipment
For the complete Syllabus, results, class timetable, and many other features kindly download the iStudy App
It is a lightweight, easy to use, no images, and no pdfs platform to make students’s lives easier..
Learning Materials
- Deb & Deb Fundamental of Biochemistry New Central Agency. Kolkata, ISBN 13:9788173811449
- Ramkrishna, Prasanna and Rajan Text Book of Biochemistry Orient Longman Ltd, ISBN 978825020714
- Rama Rao Text Book of Biochemistry UBS Publication ISBN 10:817476660X
- P.B Godkar and D.P Godkar Text Book of Medical Laboratory Technology Bhalani Publishing House, Mumbai-12 ISBN: 9789381496190
- J.L Jain Fundamental of Biochemistry S.Chand &CO ISBN: 9788121924535
Learning Websites
- https://www.youtube.com/watch?v=CB7CbV1Cnh0 Isolation of DNA from liver sample
- https://www.youtube.com/watch?v=keRjfFSWaaY Identification of DNA by Diphenylamine reagent.
- https://www.youtube.com/watch?v=18bFdY7_Dno Isolation of RNA by Yeast Cell.
- https://www.youtube.com/watch?v=RmPsLoIPRwc Detection of RNA from Trizol reagent.
- https://www.youtube.com/watch?v=JNl1kjw9ZDQ Separation of RNA from protein by Phenol extraction method
- https://www.youtube.com/watch?v=3dbQIr0q9sc Estimation of RNA by Orcinol method.
- https://www.youtube.com/watch? v=D_NCevZ76UI&t=45s Measurement of alkaline phosphatase in serum
- https://www.youtube.com/watch? v=dbDMgKyTeNM&t=29s Measurement of SGPT activity
- https://www.youtube.com/watch?v=9jOMvA-jdDk Measurement of SGOT activity
- https://www.youtube.com/watch?v=jL56wDIzpmE Isolation of amylase enzyme form saliva
For detail Syllabus of all other subjects of Medical Laboratory Technology, K scheme do visit Medical Laboratory Technology 5th Sem Syllabus for K scheme.
For all Medical Laboratory Technology results, visit MSBTE Medical Laboratory Technology all semester results direct links.