Histotechnique detailed Syllabus for Medical Laboratory Technology (ML), K scheme PDF has been taken from the MSBTE official website and presented for the diploma students. For Subject Code, Subject Name, Lectures, Tutorial, Practical/Drawing, Credits, Theory (Max & Min) Marks, Practical (Max & Min) Marks, Total Marks, and other information, do visit full semester subjects post given below.
For all other MSBTE Medical Laboratory Technology 4th Sem K Scheme Syllabus PDF, do visit MSBTE Medical Laboratory Technology 4th Sem K Scheme Syllabus PDF Subjects. The detailed Syllabus for histotechnique is as follows.
Rationale
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Course Outcomes:
Students will be able to achieve & demonstrate the following COs on completion of course based learning
- Gross the tissue obtained by biopsy or autopsy
- Select the appropriate fixatives for fixation of tissue
- Check completion of decalcification process of given tissue
- Prepare paraffin block of given tissue using appropriate technique
- Prepare the histological slides of given sample
Unit I
Histopathology 1.1 Introduction and Importance of histopathology and cytopathology 1.2 Arrangement and working of histopathology laboratory, perform various histotechnique 1.3 Definition, structure and function of cell and tissue 1.4 Different methods of specimen collection for Histotechniques and grossing of it 1.5 Safety precaution in Histotechniques
Suggested Learning Pedagogie
Video Demonstrations Flipped Classro Presentations Lecture Using C Board
Unit II
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Unit III
Decalcification 3.1 Definition, properties and types of decalcifying fluids 3.2 Study of advantages and disadvantages of the decalcifying agent 3.3 Detection of end point of decalcification by completion test 3.4 Various chemicals and reagent requires for checking of decalcification 3.5 Precaution in decalcification
Suggested Learning Pedagogie
Presentations Video Demonstrations Lecture Using C Board Collaborative learning
Unit IV
Tissue Processing 4.1 Dehydration – Aim, agents and its Properties 4.2 Clearing – Aim, agents and its Properties 4.3 Impregnation and infiltration media 4.4 Embedding – Block preparation and storage 4.5 Automatic tissue processor – construction and use
Suggested Learning Pedagogie
Presentations Video Demonstrations Lecture Using C Board
Unit V
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List of Experiments:
- Arrangement and instruments and chemical/ reagent in histopathology laboratory 4 C
- Fixation of obtained sample by Biopsy/ Autospy 4 C
- Decalcification process and end point 4 C
- Dehydration – different aspects and procedure 4 C
- Clearing various agents and process 4 C
- Impregnation – Proper media and duration 4 C
- Embedding : Paraffin block preparation 4 C
- Treaming and storage of tissue embedded block 4 C
- Sharpening and microtome knief by honing and stropping 4 C
- Microtomy actual section cutting 4 C
- Mounting of section ( floating out techniques) 4 C
- Use, care and precaution of rotary microtome 4 C
- Haematoxylene and Eosin staining 4 C
- Permanent mounting by using DPX and deserrtative of slide 4 C
- Smear preparation of gynacological and nongynacological samples 4 C
- Popanicolaou staining 4 C
Self Learning
Micro Project
- Prepare flow diagram of Histotechniques
- Prepare charts of microscope and microtome
- Survey for cancer patients
- Design an effective campaigning poster for awareness regarding cancer of various organs in English Hindi marathi languages
Laboratory Equipment
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Learninig Materials
- Baker F.J. R. E. Silverton Introduction to Medical laboratory Technology EIBS London 5th edition 1980-82
- Medical college Nagpur Manual of Histopathology Students store GMC Nagpur
- By L. G. Koss Diagnostic Cytology Vol. I & II J. B. Lippincott Co. Philadelphia 3rd Edition 19
- By Monica Cheesbrough Medical lab Manual for tropical countries EIBS London 1987
- By Kanai Mukherjee Medical Lab Technology Vol. I, II, III Tata N. C. Graw Hill 1988
- Sadhana Vishwakarma Techniques in histopathalogy and cytopathalogy Jaypee Brothers Medical Publishers Pvt. Limite 2017 9352701097, 9789352701094
Learning Websites
- https://youtu.be/Hf1-CLm0u08?si=rRjKwmhXR7CP4atY Introduction of cytopathology
- https://youtu.be/4DJm4NLECQs?si=dSCQEI-w8SQSyEP5 Histology technique and equipment
- https://youtu.be/xSgHVy62ckc?si=XvIpfoW-cZaxelYG Tissue processing
- https://youtu.be/7-LIbAWPc-g?si=J9QKV0ZSSHKgbsg_ Tissue processing , Embedding, section histology
- https://youtu.be/vln689JrssQ?si=jfthXoCRJktD5Jc7 Fixative and types of fixatives
- https://youtu.be/OAATyrRNPQU?si=lFNphvINn7Gn0xNE Decalcification
- https://youtu.be/MslMX5JNXGM?si=gkv9452pkcbRFMe6 Types of Decalcification
- https://youtu.be/1jgY-u6u-j4?si=V3q8Cmh932ISXWRT Microtome
- https://youtu.be/2D0rj0m6dVs?si=XCiFlXlBb-vHT5RH H and E staining
- https://youtu.be/KnMdSgd5mts?si=0psKWcAYBtidI0xx Rotary microtome section
- https://youtu.be/MWHxq_Xly-A?si=xxb2sFTsrLgabMcj Microtomy, Block cutting
For detail Syllabus of all other subjects of Medical Laboratory Technology, K scheme do visit Medical Laboratory Technology 4th Sem Syllabus for K scheme.
For all Medical Laboratory Technology results, visit MSBTE Medical Laboratory Technology all semester results direct links.