322304: Medical Laboratory Instruments Syllabus for Medical Laboratory Technology 2nd Sem K Scheme MSBTE PDF

Medical Laboratory Instruments detailed Syllabus for Medical Laboratory Technology (ML), K scheme PDF has been taken from the MSBTE official website and presented for the diploma students. For Subject Code, Subject Name, Lectures, Tutorial, Practical/Drawing, Credits, Theory (Max & Min) Marks, Practical (Max & Min) Marks, Total Marks, and other information, do visit full semester subjects post given below.

For all other MSBTE Medical Laboratory Technology 2nd Sem K Scheme Syllabus PDF, do visit MSBTE Medical Laboratory Technology 2nd Sem K Scheme Syllabus PDF Subjects. The detailed Syllabus for medical laboratory instruments is as follows.

Rationale

Medical laboratory instruments play a diagnostic role in modern pathology laboratories. This course exposes the students to medical laboratory standard protocols and quality control measures so that they will implement these for conducting various tests on samples of blood and urine.

Course Outcomes:

Students will be able to achieve & demonstrate the following COs on completion of course based learning

1. Follow safety guidelines, standard protocol, and quality control measures in the medical laboratory.
2. Operate basic laboratory instruments such as PH meter, Glucometer, Autoclave, Hot air oven etc.
3. Analyse blood,Urine,protein samples using colorimeter and Spectrophotometer.
4. Handle semi and fully automated flame photometer and analyzers in Hi-tech laboratories.
5. Estimate Protein and DNA from different specimens using Electrophoresis and Chromatography.

Unit I

Basic Laboratory Techniques 1.1 Types of Containers- Blood , Sputum , Urine , Stool. 1.2 Collection of – Blood , Sputum , Urine , Stool. 1.3 Transportation and Disposal of – Blood , Sputum , Urine , Stool. 1.4 Define Quality control and Quality management, Types of Quality control ,Quality control chart and lab safety chart ( Leavy Jenning and Cusum chart).
Suggested Learning Pedagogy Demonstration Model Demonstration Video Demonstrations Site/Industry Visit Chalk-Board

Unit II

Basic Laboratory Instruments 2.1 Instrument- PH Meter , Glucometer. 2.2 Instrument- Wet Heat Sterilizer (Above 100°C) , Dry Heat Sterilizer. 2.3 Instrument- Laminar Air Flow (LAF) , Incubator 2.4 Centrifuge- Components , Principle , Working , Care And Uses.
Suggested Learning Pedagogy Chalk-Board Demonstration Video Demonstrations Model Demonstration Site/Industry Visit

Unit III

Colorimeter & Spectrophotometer 3.1 Definition of spectroscopy 3.2 UV and visible range (wave length) 3.3 Beer`s and Lambert`s Law (only statement) 3.4 Colorimeter, Spectrophotometer – Principle, Components, Working, care and uses
Suggested Learning Pedagogy Chalk-Board Demonstration Video Demonstrations Presentations Site/Industry Visit Flipped Classroom

Unit IV

Flame Photometer & Analyzer 4.1 Definition of Photometer 4.2 Flame Photometer – Ray diagram, Principle, Components, Care and uses 4.3 Analyzer (Semi and Auto)- Ray diagram, Principle, Components, Care, Maintenance and uses
Suggested Learning Pedagogy Chalk-Board Video Demonstrations Demonstration Site/Industry Visit Flipped Classroom Presentations

Unit V

Electrophoresis & Chromatography 5.1 Definition of Electrophoresis, Chromatography. 5.2 Gel Electrophoresis – Diagram, Principle, components, Working, Care and Uses 5.3 Thin Layer Chromatography , Paper Chromatography – Diagram, Principle, components, Working, Care and Uses
Suggested Learning Pedagogy Chalk-Board Video Demonstrations Model Demonstration Presentations Flipped Classroom

Practical / Tutoria Experiences

1. Draw diagrams of different containers*
2. Perform collection and transportation of blood accurately*
3. Collect and label urine sample accurately*
4. Perform disposal of biomedical waste by various physical methods *
5. Perform disposal of biomedical waste by various chemical methods *
6. Prepare quality control chart from given data (Leavy jenning & Cusum chart) *
7. Separation of plasma and serum by centrifugation *
8. Identify the PH of given urine sample *
9. Perform sterilization by dry heat sterilization. *
10. Perform Contamination sensitive processes by laminar
11. Determination of blood glucose by glucometer.*
12. Determine optical density of given solution by colorimeter.*
13. Identify the different components in sample by analyzer.*
14. Perform separation of urine components by TLC
15. Demostration of working of flame photometer.
16. Demonstration of working of Gel electrophoresis.

Note : Out of above suggestive LLOs – ‘*’ Marked Practicals (LLOs) Are mandatory. Minimum 80% of above list of lab experiment are to be performed. Judicial mix of LLOs are to be performed to achieve desired outcomes.

Micro Project / Self Learning

Assignment

• Prepare list of pathological laboratories in your areas.
• Prepare charts and collect information about normal and abnormal constituents of Stool.
• Digramatically show working of instruments .
• Prepare charts and collect information about normal and abnormal constituents of urine.
• Prepare charts and collect information about normal and abnormal constituents of Blood.
• Prepare charts of different laboratory Instruments.

Micro project

• Visit pathology laboratories to identify principles and techniques used there, Prepare a report.
• Arrange exhibition of lab instruments.
• Preparation of chart for different specimen containers.
• Preparation of Levey Jenning and Cusum chart.
• Survey of random blood sugar in your college.
• Participate in blood donation camp and prepare report.
• Survey of urine pH among different age groups.

Note : Prepare list of pathological laboratories in your areas .

Laboratory Equipment / Software Required

1. Containers for Blood, Urine, Sputum and Stool 1,3,2,4,5,7,8
2. Digital pH meter 8
3. Hot Air Oven 4,9
4. Autoclave 4,7
5. Colorimete 12
6. Gucometer 11
7. Chromatography Apparatus 14
8. Centrifuge 7
9. Laminar air flow 10

Learning Materials / Books

1. Praful B Godkar Darshan P Godkar Text Book of Medical Laboratory Technology Bhalani Publication House Third Edition Vol. I and II, 2018 ISBN : 978818557853
2. Gurdeep R Chatwal Sham K Anand Instrumentation methods of Chemical Analysis Himalaya Publication, 5i” Edition, 2018, ISBN-9789351420880
3. Satish Gupte The short Text Book of Medical Laboratory for Technician JYPEE Publication, Second Edition, ISBN;97893509085I8
4. J.Ochei A. Kolhatkar Medical Laboratory Science Theory and practice_ Mc Graw Hill Education, ISBN; 9780074632239
5. Ramnik Sood Medical Laboratory Methods & Interpretation Vol 1 & Vol 2 JPB, Sixth Edition ISBN-978-8184484496- Vol-2
6. Kanai L. Mukharjee Anuradha Chakravarthy Medical Laboratory Technology, Procedure Manual for Routine Diagnostic Tests Vol 1, 2,3 Me Graw Hill Education: Third Edition ISBN-9789352606801- Vol-I 9789352606818- Vol-II 9789352606815-
7. C.P.Baveja V. Baveja Textbook of Microbiology for MLT Arya Publication, 2nd Edition (2019), ISBN- 978-8178556987
8. Ranjana Chawla Biochemistry Methods and Interpretations Jaypee Brothers Medical Publishers, fourth Edition ISBN- 978-93 SQ,
9. Maheshwari Nanda Clinical Biochemistry Jaypee Brothers Medical Publisher, Second Edition (2017) ISBN- 978-9386150196
10. Dr. Avinash Upadhyay Dr. Kakoli Upndhyny Dr. Nirmalendu Nath Biophysical Chemistry- Principal and technique Himalaya Pub1. House Pvt. Ltd. Fourth Edition ISBN-978-9951422273

Learning Websites & Portals

1. https://microbiologyinfo.com/good-laboratory-practices To maintain quality control
2. https://en.wikipedia.org/wiki/Spectrophotometry Principle And application of spectrophotometer
3. https://www.microlit.us/what-are-the-different-types-of-medi cal-lab-instruments/ study of basic lab instruments
4. https://en.wikipedia.org/wiki/Instruments_used_in_medical_la boratories Instruments use in medical laboratories
5. https://www.itm.edu/blog/ihs-list-of-main-tools-used-by-mlt-professionals to study analyzer
6. https://www.labkafe.com/blog/laminar-airflow-0522 Laminar air flow
7. https://www.differencebetween.com/difference-between-electro phoresis-and-vs-chromatography/ Chromatography and Electrophoresis

For detail Syllabus of all other subjects of Medical Laboratory Technology, K scheme do visit Medical Laboratory Technology 2nd Sem Syllabus for K scheme.

For all Medical Laboratory Technology results, visit MSBTE Medical Laboratory Technology all semester results direct links.