321004: Basic Laboratory Practices Syllabus for Medical Laboratory Technology 1st Sem K Scheme MSBTE PDF

Basic Laboratory Practices detailed Syllabus for Medical Laboratory Technology (ML), K scheme PDF has been taken from the MSBTE official website and presented for the diploma students. For Subject Code, Subject Name, Lectures, Tutorial, Practical/Drawing, Credits, Theory (Max & Min) Marks, Practical (Max & Min) Marks, Total Marks, and other information, do visit full semester subjects post given below.

For all other MSBTE Medical Laboratory Technology 1st Sem K Scheme Syllabus PDF, do visit MSBTE Medical Laboratory Technology 1st Sem K Scheme Syllabus PDF Subjects. The detailed Syllabus for basic laboratory practices is as follows.

Rationale

This course is intended to develop students’ skills in the proper handling and utilization of glassware, chemical, and instrument in medical laboratories.

Course Outcomes:

Students will be able to achieve & demonstrate the following COs on completion of course based learning

1. Select glassware and reagents according to identified procedure/test.
2. Handle electronic instrument effectively for test and measurement.
3. Preserve microbial cultures as per standard procedure.
4. Maintain the specimen as per standard procedure.
5. Dispose biological waste by following standard norms.

Unit I

Lab Practices in Biochemistry 1.1 Glassware – Types, Uses, Cleaning and Handling 1.2 Reagent-Type, Handling 1.3 Unit and Measurement, Mass, Volume, Concentration, Percent, density, ionic strength 1.4 Calibration of instruments – electronic balance, pH meter, Pipette.
Suggested Learning Pedagogy Demonstration Flipped Classroom Collaborative learning Hands-on Site/Industry Visit

Unit II

Lab Practices in Microbiology 2.1 Microscope- Parts and care 2.2 Preparation of media and sterilization technique 2.3 Aseptic Technique 2.4 Pure culture techniques 2.5 Culture preservation
Suggested Learning Pedagogy Demonstration Flipped Classroom Hands-on Collaborative learning Site/Industry Visit

Unit III

Lab Practices in Pathology 3.1 Specimen – Types, Collection and processing 3.2 Instruments in pathology – Name and uses 3.3 Diagnostic Test – List and objective of test 3.4 Record keeping in pathology 3.5 Reporting – Guidelines for disclosure
Suggested Learning Pedagogy Video Demonstrations Demonstration Flipped Classroom Hands-on Site/Industry Visit

Unit IV

Lab Practices in Hematology 4.1 Types of Blood Samples 4.2 Anticoagulants 4.3 Collection of blood 4.4 Precautions during blood collection 4.5 Instruments in hematology
Suggested Learning Pedagogy Demonstration Flipped Classroom Hands-on Site/Industry Visit Collaborative learning

Unit V

General Laboratory Practices 5.1 Safety Practices, Causes of accidents, General safety rules, Safety signs and symbols. 5.2 First Aid 5.3 Fire, Causes of Fire, Basic ways of extinguishing the fire, Classification of fire, Class A, B, C, D, Firefighting equipment, fire extinguishers and their types 5.4 Biomedical waste – Types (Sharp waste, Infectious waste, Pathological waste) 5.5 Risk Associated with health-care waste – Types of hazards, Persons at risk, Hazards from infectious waste and sharps. 5.6 Health-care waste minimization ,reuse and recycling
Suggested Learning Pedagogy Video Demonstrations Demonstration Flipped Classroom Hands-on Site/Industry Visit Collaborative learning

Practical / Tutoria Experiences

1. Measure the given solution accurately
2. Weigh the given solid sample accurately
3. Separate the mixture of solution accurately
4. Filter the given solution using standard procedure.
5. Prepare 1N solution of NaOH as per standard procedure
6. Clean the glassware (Test tube, conical flask, beaker) effectively
7. Draw a well labeled diagram of compound microscope accurately
8. Identify different parts of compound microscope accurately
9. Perform Cleaning of the given microscope as per standard procedure
10. Perform sterilization of nichrome wire loop as per standard procedure
11. Perform disinfection of laminar hood as per standard procedure
12. Perform disinfection of hand effectively
13. Draw neat well labeled diagram of different sample containers effectively
14. Collect urine sample accurately
15. Process tissue specimen accurately
16. Label the different specimen accurately
17. Prepare a chart of test performed in pathology with their objectives as per standard procedure
18. Prepare a record of different specimen as per standard procedure
19. Prepare a report of pathological investigation accurately
20. Detect the vein for collection of blood accurately
21. Prepare bulb for collection blood as per standard procedure
22. Identify different blood collection site and devices accurately
23. Perform collection of blood sample accurately
24. Perform separation of serum accurately
25. Perform separation of plasma accurately
26. Perform mock drill session in group of minimum 10 students for extinguishing fire as per standard procedure
27. Prepare first aid box as per standard procedure
28. Categorize biomedical waste as per standard norms
29. Perform disposal of biomedical waste as per standard procedure
30. Perform recycling of biomedical waste accurately

Note : Out of above suggestive LLOs – ‘*’ Marked Practicals (LLOs) Are mandatory. Minimum 80% of above list of lab experiment are to be performed. Judicial mix of LLOs are to be performed to achieve desired outcomes.

Micro Project / Self Learning

Assignment

• Prepare a list of pathology laboratories in your area
• Prepare a list of multispecialty hospitals
• Prepare a list of medical colleges near you
• Prepare list of blood bank near you

Term Work

• Prepare journal of practical.

Micro project

• Prepare a chart of different types of chemicals
• Prepare first aid box
• Prepare model of Pathology Lab

Laboratory Equipment / Software Required

1. Compound Microscope, Binocular Microscope 7,8,9
2. Test Tube, Measuring Cylender, Beaker, Conical Flask, Pipettes and other glasswares 1,2,3,4,5,6,14,15,16,23
3. Electronic balance 1,2,3,4,5,6
4. Anticoagulant bulb, tubes torniquet 20,21,22,23,24,25

Learning Materials / Books

1. Praful B. Godkar , Darshan P. Godkar Textbook Of Medical Laboratory Technology Clinical Laboratory Science And Molecular Diagnosis Bhalani Publishing House ISBN-13 978-9381496190
2. Barbara H. Estridge Anna P. Reynolds Basic Clinical Laboratory Techniques Sixth Edition Delmar Cengage Learning ISBN-13:978-1-1111-3836-3
3. J. Vandepitte and J Verhaegen Basic Laboratory Procedures in clinical Bacteriology 2 nd edition World Health Organization 2003

Learning Websites & Portals

1. https://www.researchgate.net/publication/344453061_BIOCHEMIS TRY_LABORATORY_SAFETY_RULES_FOR_UNDERGRA Biochemistry lab practice rules
2. Centers for Disease Control. Learning
3. CDC – Division of Laboratory Sciences Learning
4. Clinical and Laboratory Standards Institute (CLSI) Learning
5. Commission on Office Laboratory Accreditation (COLA) Learning
6. National Institute for Standards and Technology (NIST) Learning
7. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3001836/ Good Laboratory Practices
8. https://egyankosh.ac.in/bitstream/123456789/76755/3/Unit-1.p df Introduction to biochemistry laboratory
9. https://microbiologyinfo.com/good-laboratory-practices/ Good laboratory practices microbiology
10. https://www.youtube.com/watch?v=rhTh8LYYBcQ Introduction to Biochemistry Laboratory Equipments and Safety Measures
11. https://onlinecourses.nptel.ac.in/noc23_bt56/preview Experimental Biotechnology
12. https://www.youtube.com/watch?v=6-oTnYOvhIA Good lab practices in microbiology
13. https://bio.libretexts.org/Courses/North_Carolina_State_Univ ersity/MB352_General_Microbiology_Labora Safety rules for microbiology laboratory
14. https://www.youtube.com/watch?v=nZCse7vKyiM NABL rules for record keeping
15. https://www.indiamart.com/proddetail/pathology-management-so ftware-22926611612.html Record Keeping software
16. https://www.youtube.com/watch?v=jrWD83b8hkA Anticoagulants in laboratory
17. https://www.youtube.com/watch?v=oVd5Qf5eNnw Blood sample collection tubes
18. https://www.youtube.com/watch?v=eGIwrAeG5-s https://www.youtube.com/watch? v=GeB0qcNLrtc
19. https://www.youtube.com/watch?v=GeB0qcNLrtc Biomedical waste management
20. https://vikaspedia.in/energy/environment/waste-management/bi o-medical-waste-management Biomedical waste management

For detail Syllabus of all other subjects of Medical Laboratory Technology, K scheme do visit Medical Laboratory Technology 1st Sem Syllabus for K scheme.

For all Medical Laboratory Technology results, visit MSBTE Medical Laboratory Technology all semester results direct links.