Analytical Equipment detailed Syllabus for Medical Electronics (MU), K scheme PDF has been taken from the MSBTE official website and presented for the diploma students. For Subject Code, Subject Name, Lectures, Tutorial, Practical/Drawing, Credits, Theory (Max & Min) Marks, Practical (Max & Min) Marks, Total Marks, and other information, do visit full semester subjects post given below.
For all other MSBTE Medical Electronics 4th Sem K Scheme Syllabus PDF, do visit MSBTE Medical Electronics 4th Sem K Scheme Syllabus PDF Subjects. The detailed Syllabus for analytical equipment is as follows.
Rationale
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Course Outcomes:
Students will be able to achieve & demonstrate the following COs on completion of course based learning
- Use basic analytical equipment.
- Operate different types of sterilizing and radiochemical equipment.
- Measure the quantity of different components in blood using analytical instruments.
- Quantify pH and conductivity of blood fluid.
- Measure different pollutants present in the environment.
Unit I
Basic Analytical Equipment 1.1 Photometry laws: Beer’s and Lambert’s Law, deviation from Beer’s law, quantitative analysis and choice of wavelength 1.2 Elements of analytical instrument 1.3 Steps of calibration of analytical instruments 1.4 Colorimeter: Principle, block diagram, medical application and technical specification 1.5 Spectrophotometer: Working principle, block diagram, medical application and technical specification of single and dual beam spectrophotometer 1.6 Flame photometer: Working principle, block diagram, medical application and technical specification 1.7 Autoanalyzer: Working principle, block diagram, medical application and technical specification
Suggested Learning Pedagogie
Lecture using Chalk-Board Video Demonstratio Demonstratio
Unit II
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Unit III
Blood Cell Counter, Electron Microscope and Blood Gas Analyzer 3.1 Blood cell counter: Electro-conductive and dark field method 3.2 Chromatography: Definition, principle of chromatography, gas and liquid chromatography 3.3 Electrophoresis: Definition, principle, capillary electrophoresis, polyacrylamide gel electrophoresis (PAGE) 3.4 Transmission electron microscope (TEM), scanning electron microscope (SEM) 3.5 Blood gas analyzer: Block diagram, medical application and technical specification, circuit diagram for computation of bicarbonate, total CO2 and base excess
Suggested Learning Pedagogie
Lecture using Chalk-Board Video Demonstratio Flipped Classroom
Unit IV
pH and Conductivity Meter 4.1 pH meter, null- detector type and direct reading pH meter 4.2 Measurement of conductance using null method and direct method, 2-electrode conductivity sensor, 4-electrode conductivity sensor, inductive conductivity sensor 4.3 Beat frequency method for conductivity measurement 4.4 Equivalent circuit diagram of conductivity cell 4.5 Temperature compensation of conductivity measurement
Suggested Learning Pedagogie
Lecture using Chalk-Board Flipped Classroom Presentations
Unit V
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List of Experiments:
- *Colorimeter usage to measure the concentration of the solution 2 C
- Spectrophotometer usage to measure the concentration of the solution 2 C
- Flame photometer usage to measure the concentration of the solution 2 C
- *Auto-analyzer usage to measure the concentration of different components in the blood 2 C
- *Separation of blood serum and plasma using a centrifuge 2 C
- Sterilization of micro components using ultrasonic cleaner 2 C
- Sterilization of the given tools using a hot-air oven 2 C
- *Sterilization of instruments, lab ware and biological waste using an autoclave 2 C
- Biomedical waste disposal using an incinerator machine 2 C
- *Application of electrophoresis apparatus to separate the bioparticles 2 C
- Blood cell counter usage to measure WBC, RBC and platelets in the blood 2 C
- *Interpretation of sample contents using a microscope. 2 C
- and pH using Op-amp circuit 2 C
- *pH meter usage to measure the pH of a given solution 2 C
- Measurement of conductivity of the solution using conductivity meter 2 C
- *Air pollutant analysis 2 C
Self Learning
Micro Project
- Collect information about the RTPCR testing kit for COVID- 19 and make a detailed report on it.
- Design a working model for the centrifuge.
- Design electronic circuits to build ultrasonic cleaner.
- / pO2 /pH using op-amp circuits. Make a report.
- Collect the information about the genetic analyzer and prepare a powerpoint presentation.
- Collect the information about blood collection monitor and prepare a powerpoint presentation.
- Make a chart showing a comparative study of commonly used sterilizing equipment.
- Find out the specifications of analytical instruments from various manufacturers. Prepare comparative state of different analytical instruments along with their specifications.
Assignment
- Compare single and dual-beam spectrophotometers.
- Describe different sterilizing equipment.
- Compare electrophoresis and chromatography techniques.
- Draw the equivalent circuit diagram of the conductivity cell.
- Give the ideal range of concentration of gases in the environment like carbon monoxide, hydrocarbons, sulp dioxide, nitrogen dioxide, and ozone to keep the environment pollutant-free.
Hospital / Industry Visit
- Visit the nearby pathology laboratory and prepare a detailed report on the advanced microscope used.
- Visit a nearby medical industry/pathology laboratory/ hospital to observe the maintenance steps of analytica equipment.
- Visit any blood blank and prepare a detailed activity report.
- Visit any PUC centre and prepare a report on it.
- Visit a nearby pathology laboratory and observe the features of an automated enzyme-linked immunosorben assay(ELISA).
- Visit a nearby pathology laboratory and prepare a detailed report on the advanced microscope used.
Laboratory Equipment
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Learning Materials
- Khandpur, R. S. Handbook of Analytical Instrumentation McGraw-Hill Professional, edition, 2015, ISBN: 978-9339221355
- Veerakumari, L. Bioinstrumentation MJP Publishers,1st edition, ISBN: 978-8180940187
- Natarajan, R. Biomedical Instrumentation and Measurements PHI learning Pvt. Ltd, 2nd edition, ISBN: 978-8120352155
- Goswami, Chinmoy; Paintal, Abhijit; Narain, Rabindra. Handbook of Bioinstrumentation Dominant Publishers & Distributors, ISBN: 978-9380199788
- Goswami, Chinmoy; Paintal, Abhijit; Narain, Rabindra. Handbook of Laboratory Instrumentation Wisdom Press, ISBN: 978-9380199719
- Khandpur, R. S. Handbook of Biomedical Instrumentation McGraw Hill Education,3r edition 2014, ISBN: 978-9339205430
Learning Websites
- https://youtu.be/qIW_VjVf3ZY?si=1s_f1wKwuW4SNunE Elecrtrophoresis
- https://youtu.be/lrgMPIbL7yM Basics of laboratory researc NPTL video
- https://www.digimat.in/nptel/courses/video/102107028/L01.htm l Analytical technologies
- http://www.digimat.in/nptel/courses/video/103108100/L07.html Modern instruments for chemical analysis
- https://youtu.be/ZK4KEA2o0WY Analytical methods of air pollutant measurment
- https://youtu.be/XD1W4Ak01b4 Autoanalyser
- https://youtu.be/BM9qQ_sHWP8 basic concept of electrophor
- https://youtu.be/LRG7djw_2YY?si=fe1JeRBev679IPeF blood cell counter
- https://youtu.be/ig6F71oFRA8 lab instrument interfacing
For detail Syllabus of all other subjects of Medical Electronics, K scheme do visit Medical Electronics 4th Sem Syllabus for K scheme.
For all Medical Electronics results, visit MSBTE Medical Electronics all semester results direct links.