22435: Analytical Equipment Syllabus for Medical Electronics 4th Sem I – Scheme MSBTE

Analytical Equipment detailed Syllabus for Medical Electronics (MU), I – scheme has been taken from the MSBTE official website and presented for the diploma students. For Subject Code, Subject Name, Lectures, Tutorial, Practical/Drawing, Credits, Theory (Max & Min) Marks, Practical (Max & Min) Marks, Total Marks, and other information, do visit full semester subjects post given below.

For all other Diploma in Medical Electronics (MU) Syllabus for 4th Sem I – Scheme MSBTE, do visit Diploma in Medical Electronics (MU) Syllabus for 4th Sem I – Scheme MSBTE Subjects. The detailed Syllabus for analytical equipment is as follows.

Rationale:

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Competency:

The aim of this course is to help the student to attain the following industry identified competency through various teaching learning experiences:

• Operate various analytical equipment.

Course Outcomes:

The theory, practical experiences and relevant soft skills associated with (his course are to be taught and implemented, so that the student demonstrates the following industry oriented COs associated with the above mentioned competency:

1. Use basic analytical equipment.
2. Operate different types of centrifuges and sterilizing equipment.
3. Measure different types of components of blood using analytical instruments.
4. Quantify pH and conductivity of body fluid.
5. Measure the different pollutants present in the environment.

Suggested Exercises:

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Instruments Required:

The major equipment with broad specification mentioned here will usher in uniformity in conduct of experiments, as well as aid to procure equipment by authorities concerned.

Equipment Name with Broad Specifications

1. Colorimeter : Automatic cooling.6-7 tests per hour, fixed bomb cylinder with removable head for fast sample loading, operator time per test is 1 minute,0 1% precision class instrument.0 0001 c temperature resolution,5000 – 8000 calorie sample range,0 05% linearity across operating range.
2. Spectrophotometer: wavelength 190 to 1 100 nm,baseline stability less than 0 0003 Abs/11 at 700 nm (one hour after light source turned ON), Baseline flatness: within 0 0006 Abs (190 to 1100nm,one hour after light source turned ON), Noise level: Within 0 00005 Abs RMS value (at 700 nm.
3. Flame photometer: Filters: Na and K (Ca and Li optional), Range: Na: 0-100 ppm, Ca: 20-100 ppm, K : 0-100 ppm, Li: 10-100 ppm, Sensitivity: Na: 5 ppm, Ca: 10 ppm, K : 5 ppm, Li: 10 ppm
4. Ultrasonic Cleaner: Capacity:! .3 to 14 Liter Tank, Ultrasonic Cleaning Powcr:60W to 750W, Ultrasonic frequencies: 20KIIz / 40KIIz, Digital timer adjustment: 1-99 minutes, Digital temperature adjustment: 0-70 C, Stainless Steel Basket and cover lid. Tank and housing made from Stainless Steel.
5. Centrifuge : max capacity: 3 0 L, Refrigerated and constant controlled models, 10,200 RPM, 11 400 x g,ARIES Smart Balance Rotor System
6. Hot air oven: Temperature Range: 5C above ambient to 250C maximum, Temperature Accuracy: + / – 2C, Temperature Uniformity: / – 1C, Controls: PID Controller, Sensor: PT-100 Kanthal Al, Heating Element: Nichrome wire
7. Autoclave : Electrical Power: 18 KW or Sufficient wattage of industrial immersion type water heater to generate steam within a reasonable period of time on 3 phase 440V 50 HZ AC supply, Working pressure and Temperature : 1 2 to 2 2 Kg/sq.cm at 121 Deg C, Material of Construction Inner chamber , Jacket, Door : SS 316.(5mm-10mm), Outer Chamber : SS 304 (Insulated properly), Steam Generator : Non corrosive SS / Chromium plated Brass, Heater Plate : Brass / Stainless Steel
8. Auto analyzer: Auto sampler, peristaltic pump, nitrate manifold designed for auto analyzer used, single channel colorimeter equipped with 15 mm flow cell and 550 nm filter /rpl 8 5 pm, 250 mm x 4 mm chromatographic hplc column, sampler- random access sampler with throughput of more than 150 samples in cups or tubes, manifold- should include applications for hydrogen cyanide measurements, flow cell- should be having bubble through the flow cell operation, colorimeter should be suitable for hydrogen cyanide analysis.
9. Blood cell counter: 18 parameters with 3 part differential, 1000 samples storage with histogram, touch screen operation, built-in self-monitoring system, low cost per test.
10. Blood gas analyzer: Essential Measured parameters: pH, pCO2, pO2, Hb, Barometric Pressure Na+, K.+, Ca, Cf. All these parameters should be measured simultaneously . Calculated parameters should include BE, BE ecf, FICO3, Lactate, Anion Gap, SaO2 etc, Sample volume: less than lOOul, Fast analysis time: less than 60 sec
11. Air quality detector: Power: Rechargeable NiMH battery pack: 18-24 hours of continuous operation time (dependent upon the sensor array installed) c/w plug-in battery charger / wall adapter (12 VDC or 100 – 240 VAC), Sample : Internal, automatic sample pump for active” sampling of target environment. Operating Ranges: Temperature: 5C to 50C (41 F to 122F); Relative Humidity: 0 – 99% RH non-condensing. Sensors: Carbon Monoxide (CO), Carbon Dioxide (CO?), Sulphur Dioxide (SO?). Ozone (0?), Oxygen (O2)

Unit 1

Laboratory Equipment

Total Teaching Hours – 10

Distribution of Theory Marks

R Level – 04

U Level – 04

A Level – 06

Total Marks – 14

Unit Outcomes (UOs) (in cognitive domain)

1. Identify composition of the given material with the help of Beers and Lamberts law with justification.
2. Describe with sketches the function of the specified element of a generalized analytical instrument with a sketch.
3. Describe the steps for calibration of the given analytical equipment.
4. Describe with sketches the functions of the given parts of the specified analytical equipment.
5. Identify the analytical equipment for the given application with justification.
6. Explain with sketches the operating principle of the given analytical instrument along with its technical specifications.

Topics and Sub-topics

1. Photometry laws (Beers and Lamberts Law), deviation from Beers law, quantitative analysis and choice of wavelength
2. Elements of analytical instruments
3. Colorimeter
4. Spectrophotometer – Single and dual beam
5. Flame photometer
6. Auto analyzer

Unit 2

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Unit 3

Blood Gas Analyzer, Blood Cell Counter and Electron Microscope

Total Teaching Hours – 10

Distribution of Theory Marks

R Level – 04

U Level – 04

A Level – 06

Total Marks – 14

Unit Outcomes (UOs) (in cognitive domain)

1. Describe the procedure to identify and quantify the given dissolved gas present in blood.
2. Describe the procedure to measure the amount of specified blood cell.
3. Describe with sketches the construction ofthe given equipment used to measure dissolved gases and cells present in blood.
4. Describe with sketches the procedure of separation technique used for the specified application.
5. Describe with sketches the construction ofthe given chromatography/ electrophoresis equipment.
6. Identify in the given figure the parts of electron microscope with its function.

Topics and Sub-topics

1. Measurement of pH. pO2, pCO2, HCO2, TCO2, base excess using blood gas analyzer.
2. Electro- Conductive blood cell counter, Dark Held blood cell counter
3. Definition, principle of Chromatography and electrophoresis
4. Classification of Chromatography: Gas and Liquid Chromatography
5. Capillary electrophoresis
6. Polyacrylamide gel electrophoresis (PAGE)
7. TEM (Transmission Electron Microscope)
8. SEM (Scanning Electron Microscope)

Unit 4

pH and Conductivity meter

Total Teaching Hours – 12

Distribution of Theory Marks

R Level – 04

U Level – 06

A Level – 08

Total Marks – 18

Unit Outcomes (UOs) (in cognitive domain)

1. Describe with sketches the construction of equipment used to measure pH of the given solution.
2. Describe with sketches of the specified sensor along with the method used for measuring the pH and conductivity of given solution.
3. Interpret high frequency circuit of the given conductivity cell.
4. Recommend the relevant method to improve the accuracy of the given type of conductivity meter with justification.

Topics and Sub-topics

1. pH meter, Null method and direct method for conductivity measurement
2. 2-electrode conductivity sensor, 4-electrode conductivity sensor, Inductive conductivity sensor
3. Beat frequency method
4. Equivalent circuit diagram of conductivity cell
5. Temperature compensation of conductivity measurement

Unit 5

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Suggested Student Activities:

Other than the classroom and laboratory learning, following are the suggested student-related co-curricular activities which can be undertaken to accelerate the attainment of the various outcomes in this course: Students should conduct following activities in group and prepare reports of about 5 pages for each activity, also collect/record physical evidences for their (students) portfolio which will be useful for their placement interviews:

1. Survey and present a report on details of various analytical equipment from Library / Internet.
2. Prepare power point presentation or animation for understanding operation and demonstration of analytical equipment.

Suggested Special Instructional Strategies:

These are sample strategies, which the teacher can use to accelerate the attainment of the various outcomes in this course:

1. Massive open online courses (MOOCs) may be used to teach various topics/sub topics.
2. L in item No. 4 does not mean only the traditional lecture method, but different types of teaching methods and media that are to be employed to develop the outcomes.
3. About 15-20% of the topics/sub-topics which is relatively simpler or descriptive in nature is to be given to the students for self-directed learning and assess the development of the COs through classroom presentations (see implementation guideline for details).
4. With respect to item No.10, teachers need to ensure to create opportunities and provisions for co-curricular activities.
5. Use Flash/Animations to explain various theorems in circuit analysis
6. Guide students in undertaking micro-projects

Suggested Micro-Projects

For the complete Syllabus, results, class timetable, and many other features kindly download the iStudy App
It is a lightweight, easy to use, no images, and no pdfs platform to make students’s lives easier.
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Suggested Learning Resources:

1. Flandbook of Analytical Instruments Khandpur, R. S. McGraw-Hill Education. New Delhi, 2014, ISBN: 9339221362
2. Bioinstrumentation Veerakumari, L. MIR Publishers, Moscow, 201 5 ISBN:9788180940187
3. Analytical Instrumentation: A Guide to Laboratory, Portable and Miniaturized Instruments McMahon, Gillian John Wiley & Sons. New Delhi. 2014, ISBN : 9780470027950
4. Principles of Instrumental Analysis Skoog, Douglas A.; Holler, James F.; Stanley, R. Crouch Cengage Learning, New Delhi, . 2017, ISBN: 9781337468039
5. Medical Instrumentation: Application and Design Webster, John G. John Wiley and Sons, New Delhi, 2009, ISBN: 9788126511068
6. Ewings Analytical Instrumentation Handbook Cazes, Jack CRC Press, 2005 ISBN : 9780824753481

Software/Learning Websites:

1. http://www.parrinst.com/products/oxygeii-boinb-caloiimelers/6400-aulOTnalic-isoperibol-calorimeter/specifications
2. http://www.shimadzu.com/an/molecular_spectro/uv/uvl800/uv3.html
3. www.labtronicsspectrophotometers.com/digital-flame-photometers.html
4. http://smedunia.co.in/highcleanultrasonic
5. http://centrifugebybeckman.com/?page_id=1777/?pi_ad_id=92693 587516andgclid=C Pr7i8nlltlCFUgXaAodFhkMQg
6. http://www.bionicsscientific.com/laboratory-oven/hot-air-oven.html
7. http://www.k2bw.com/gasanalyzers.htm
8. http://www.kmscl.kerala.gov.in/ratecontractspec/Autoclave%20horizontal.pdf
9. http://www.mohfw.nic.in/WriteReadData/1892s/file9-94625409.pdf
10. http://www.agdbio.com/product/pce-210-fully-aulomatic-blood-cell-counter
11. http://bmsicl.gov.in/nploads/Drugs/Blood%20Gas%20Analyser%20With%20Electrol yte.pdf
12. http://www.nptel.ac.in/courses/104104066/
13. http://www.eeproccss.com/CET/yesair-junior.html

For detail Syllabus of all other subjects of Medical Electronics, I – scheme do visit Medical Electronics 4th Sem Syllabus for I – scheme.

For all Medical Electronics results, visit MSBTE Medical Electronics all semester results direct links.