{"id":1839,"date":"2016-08-05T20:13:10","date_gmt":"2016-08-05T20:13:10","guid":{"rendered":"http:\/\/www.inspirenignite.com\/jntuk\/?p=1839"},"modified":"2016-08-05T20:13:10","modified_gmt":"2016-08-05T20:13:10","slug":"jntuk-b-tech-protein-engineering-for-r13-batch","status":"publish","type":"post","link":"https:\/\/www.inspirenignite.com\/jntuk\/jntuk-b-tech-protein-engineering-for-r13-batch\/","title":{"rendered":"JNTUK B. Tech Protein Engineering for R13 Batch."},"content":{"rendered":"<p>JNTUK B.Tech Protein Engineering gives you detail information of Protein Engineering R13 syllabus It will be help full to understand you complete curriculum of the year.<\/p><div class=\"a9916ad81d5189659b0bfae0b37c143c\" data-index=\"1\" style=\"float: none; margin:10px 0 10px 0; text-align:center;\">\n<ins class=\"adsbygoogle\"\r\n     style=\"display:block; text-align:center;\"\r\n     data-ad-layout=\"in-article\"\r\n     data-ad-format=\"fluid\"\r\n     data-ad-client=\"ca-pub-1181153414625576\"\r\n     data-ad-slot=\"9648548092\"><\/ins>\r\n<script>\r\n     (adsbygoogle = window.adsbygoogle || []).push({});\r\n<\/script>\n<\/div>\n\n<p><strong>UNIT I: INTRODUCTION TO PROTEIN STRUCTURE<\/strong><br \/>\nIntroduction; Basic structural principles: amino acids and their conformational accessibilities, Ramachandran Plot; Motifs of protein structures and their packing; Schematic and topology diagrams; Families of protein structures: alpha, alpha\/beta,beta, small etc.<br \/>\n<strong>LO:<\/strong> To give an introduction about protein structure and various parameters associated with it.<br \/>\n<strong>LA<\/strong>: Assessment by ability to demonstrate the structure of protein by various plots.<\/p>\n<p><strong>UNIT II: PROTEIN FOLDING AND ASSEMBLY<\/strong><br \/>\nProtein folding pathways in prokaryotes and eukaryotes; Single and multiple folding pathways; Protein folding of single domain and multi-domain proteins; Inclusion bodies and recovery of active proteins; Osmolyte assisted protein folding; Structure of chaperones and role of chaperones in protein folding.<br \/>\n<strong>LO<\/strong>: To study the concepts of protein folding and its pathways.<br \/>\n<strong>LA<\/strong>: Assessment by demonstrating the protein folding pathway of a given protein.<\/p>\n<p><strong>UNIT III: PROTEIN STRUCTURES DESIGN AND PREDICTION<\/strong><br \/>\nSimilar structure and function of homologous proteins; Role of multiple alignment; Homology and ab-initio method for protein structure prediction; Phage display systems; Structure based drug design and case studies, Rational protein design.<br \/>\n<strong>LO<\/strong>: To study the protein structure design and homology.<br \/>\n<strong>LA<\/strong>: Assessment by ability to identify homology between different protein structures by various methods.<\/p>\n<p><strong>UNIT IV: PROTEIN EXPRESSION\/PURIFICATION, BIOPHYSICAL CHARACTERIZATION<\/strong><br \/>\nDifferent aspects of protein expression in eukaryotes and prokaryotes. Protein separation techniques: PAGE analysis, ion-exchange, size-exclusion and affinity chromatography techniques HPLC, FPLC, LC-MS etc. Protein activity analysis; biophysical characterization.<br \/>\n<strong>LO<\/strong>: To study various techniques for the purification and expression of proteins.<br \/>\n<strong>LA<\/strong>: Assessment by ability to purify the given protein by using the above mentioned techniques.<\/p>\n<p><strong>UNIT V: PROTEIN ENGINEERING<\/strong><br \/>\nStrategies for protein engineering; Random and site-directed mutagenesis; Various PCR based strategies; Role of low-fidelity enzymes in protein engineering; Gene shuffling and Directed evolution of proteins; Protein backbone changes; Antibody engineering; All topics will deal with case studies.<br \/>\n<strong>LO:<\/strong> To study different strategies involved in protein engineering.<br \/>\n<strong>LA<\/strong>: Assessment by the ability to identify a proper strategy for a specific protein engineering.<\/p>\n<p><strong>UNIT VI: PROTEIN DATABASES<\/strong><br \/>\nDifferent databases and their uses. Protein structure on the World Wide Web.<br \/>\n<strong>LO:<\/strong> To study various protein databases and importance of world wide web.<br \/>\n<strong>LA:<\/strong> Assessment by ability to utilize the structural and functional information present in these protein databases.<\/p>\n<p><strong>TEXT BOOKS<\/strong><\/p>\n<ul>\n<li>David Whitford, Proteins: Structure and Function, Wiley press, 2005.<\/li>\n<li>Lilia Alberghina, Protein engineering in Industrial biotechnology, Harwood Academic Publishers, 2002.<\/li>\n<\/ul>\n<p><strong>REFERENCES<\/strong><\/p>\n<ul>\n<li>Carl Branden and John Tooze, Introduction to Protein structure, 2nd Ed, Garland Press, 1999.<\/li>\n<li>Alan Fersht, Structure and Mechanism in Protein Science, Freeman, 1999.<\/li>\n<\/ul>\n<p>For more information about all JNTU updates please stay connected to us on FB and don\u2019t hesitate to ask any questions in the comment.<\/p>\n<div class=\"a9916ad81d5189659b0bfae0b37c143c\" data-index=\"2\" style=\"float: none; margin:10px 0 10px 0; text-align:center;\">\n<ins class=\"adsbygoogle\"\r\n     style=\"display:block; text-align:center;\"\r\n     data-ad-layout=\"in-article\"\r\n     data-ad-format=\"fluid\"\r\n     data-ad-client=\"ca-pub-1181153414625576\"\r\n     data-ad-slot=\"8060844699\"><\/ins>\r\n<script>\r\n     (adsbygoogle = window.adsbygoogle || []).push({});\r\n<\/script>\n<\/div>\n\n<div style=\"font-size: 0px; height: 0px; line-height: 0px; margin: 0; padding: 0; clear: both;\"><\/div>","protected":false},"excerpt":{"rendered":"<p>JNTUK B.Tech Protein Engineering gives you detail information of Protein Engineering R13 syllabus It will be help full to understand you complete curriculum of the year. UNIT I: INTRODUCTION TO [&hellip;]<\/p>\n","protected":false},"author":2259,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[2],"tags":[],"class_list":["post-1839","post","type-post","status-publish","format-standard","hentry","category-syllabus"],"_links":{"self":[{"href":"https:\/\/www.inspirenignite.com\/jntuk\/wp-json\/wp\/v2\/posts\/1839","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.inspirenignite.com\/jntuk\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.inspirenignite.com\/jntuk\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/jntuk\/wp-json\/wp\/v2\/users\/2259"}],"replies":[{"embeddable":true,"href":"https:\/\/www.inspirenignite.com\/jntuk\/wp-json\/wp\/v2\/comments?post=1839"}],"version-history":[{"count":1,"href":"https:\/\/www.inspirenignite.com\/jntuk\/wp-json\/wp\/v2\/posts\/1839\/revisions"}],"predecessor-version":[{"id":1840,"href":"https:\/\/www.inspirenignite.com\/jntuk\/wp-json\/wp\/v2\/posts\/1839\/revisions\/1840"}],"wp:attachment":[{"href":"https:\/\/www.inspirenignite.com\/jntuk\/wp-json\/wp\/v2\/media?parent=1839"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/jntuk\/wp-json\/wp\/v2\/categories?post=1839"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.inspirenignite.com\/jntuk\/wp-json\/wp\/v2\/tags?post=1839"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}