JNTUK B.Tech Protein Engineering gives you detail information of Protein Engineering R13 syllabus It will be help full to understand you complete curriculum of the year.
UNIT I: INTRODUCTION TO PROTEIN STRUCTURE
Introduction; Basic structural principles: amino acids and their conformational accessibilities, Ramachandran Plot; Motifs of protein structures and their packing; Schematic and topology diagrams; Families of protein structures: alpha, alpha/beta,beta, small etc.
LO: To give an introduction about protein structure and various parameters associated with it.
LA: Assessment by ability to demonstrate the structure of protein by various plots.
UNIT II: PROTEIN FOLDING AND ASSEMBLY
Protein folding pathways in prokaryotes and eukaryotes; Single and multiple folding pathways; Protein folding of single domain and multi-domain proteins; Inclusion bodies and recovery of active proteins; Osmolyte assisted protein folding; Structure of chaperones and role of chaperones in protein folding.
LO: To study the concepts of protein folding and its pathways.
LA: Assessment by demonstrating the protein folding pathway of a given protein.
UNIT III: PROTEIN STRUCTURES DESIGN AND PREDICTION
Similar structure and function of homologous proteins; Role of multiple alignment; Homology and ab-initio method for protein structure prediction; Phage display systems; Structure based drug design and case studies, Rational protein design.
LO: To study the protein structure design and homology.
LA: Assessment by ability to identify homology between different protein structures by various methods.
UNIT IV: PROTEIN EXPRESSION/PURIFICATION, BIOPHYSICAL CHARACTERIZATION
Different aspects of protein expression in eukaryotes and prokaryotes. Protein separation techniques: PAGE analysis, ion-exchange, size-exclusion and affinity chromatography techniques HPLC, FPLC, LC-MS etc. Protein activity analysis; biophysical characterization.
LO: To study various techniques for the purification and expression of proteins.
LA: Assessment by ability to purify the given protein by using the above mentioned techniques.
UNIT V: PROTEIN ENGINEERING
Strategies for protein engineering; Random and site-directed mutagenesis; Various PCR based strategies; Role of low-fidelity enzymes in protein engineering; Gene shuffling and Directed evolution of proteins; Protein backbone changes; Antibody engineering; All topics will deal with case studies.
LO: To study different strategies involved in protein engineering.
LA: Assessment by the ability to identify a proper strategy for a specific protein engineering.
UNIT VI: PROTEIN DATABASES
Different databases and their uses. Protein structure on the World Wide Web.
LO: To study various protein databases and importance of world wide web.
LA: Assessment by ability to utilize the structural and functional information present in these protein databases.
TEXT BOOKS
- David Whitford, Proteins: Structure and Function, Wiley press, 2005.
- Lilia Alberghina, Protein engineering in Industrial biotechnology, Harwood Academic Publishers, 2002.
REFERENCES
- Carl Branden and John Tooze, Introduction to Protein structure, 2nd Ed, Garland Press, 1999.
- Alan Fersht, Structure and Mechanism in Protein Science, Freeman, 1999.
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