JNTUK B.Tech Plant Biotechnology gives you detail information of Plant Biotechnology R13 syllabus It will be help full to understand you complete curriculum of the year.
UNIT I: TISSUE CULTURE
Introduction to cell and tissue culture: Tissue culture media (composition, preparation), Initiation and maintenance of callus and cell suspension culture, Somatic embryogenesis, organogenesis, Protoplast isolation culture and fusion.
LO: To understand the basics of plant tissue culture
LA: Assessment by media preparation and maintenance.
UNIT II: TISSUE CULTURE APPLICATIONS
Production of haploids, Somaclonal variations, Germplasm conservation (Cryopreservation). Production of secondary metabolites from plant cell cultures, Processes for enhancing the production of secondary metabolites. Technology of plant cell culture for production of chemicals, bioreactors systems and models for mass cultivation of plant cells.
LO: To demonstrate the production and applications of secondary metabolites.
LA: Assessment by demonstration of different pathways.
UNIT III: PLANT TRANSFORMATION TECHNOLOGY
Agrobacterium mediated gene transfer; Agrobacterium based vectors, viral vectors and their application. Direct gene transfer methods; chemical methods, electroporation, microinjection, particle bombardment.
LO: To demonstrate the various gene transfer methodologies and applications
LA: Assessment by applying these gene transfer methods.
UNIT IV: PLANT GENETIC ENGINEERING FOR PRODUCTIVITY AND PERFORMANCE (BIOTIC STRESS & ABIOTIC STRESS)
Herbicide resistance, Insect resistance, Disease resistance, virus resistance. Abiotic stress tolerance; Drought, temperature, salt.
LO: To study the various biotic and abiotic stress.
LA: Assessment by analyzing and differentiating various types of stress.
UNIT V: MOLECULAR FARMING & INDUSTRIAL PRODUCTS
Application of Plant biotechnology for the production of quality oil, Industrial enzymes, Therapeutic Proteins, Antigens (edible vaccine) and plantibodies.
LO: To understand the applications of plant molecular farming techniques.
LA: Assessment by application of these principles in production of therapeutically important products.
UNIT VI: METABOLIC ENGINEERING
Metabolic engineering for plant primary metabolites and secondary metabolites.
LO: To demonstrate the primary & secondary metabolites and their synthesis from plants.
LA: Assessment by incorporating metabolic calculations in the production of various primary and secondary metabolites.
TEXT BOOKS
- Roberta Smith, Plant Tissue Culture: Techniques and Experiments. 2nd edition Academic Press, 2000.
- Bhojwani, S.S. and Rajdan, Plant Tissue Culture: Theory and Practice, Elsevier 2004.
REFERENCES
- Crispeels, M.J. and Sadava, D.E., Plants, Genes and Crop Biotechnology, Jones and Bartlett Publishers 2nd Edition, 2003.
- Charles Cunningham and Andrew J.R. Porter, Recombinant Proteins from Plants: Production and Isolation of Clinically Useful Compounds (Methods in Biotechnology), Humana Press, 1997.
- Bernard R. Glick and John E. Thompson, Methods in Plant Molecular Biology and Biotechnology, CRC Press, 1993.
- I. Potrykus and G. Spangenberg, Gene Transfer to Plants (Springer Lab Manual), Springer Verlag, 1997.
- John Hammond, Peter McGarvey, Vidadi Yusibov, Plant Biotechnology: New Products and Applications, Springer Verlag, 1999.
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