JNTUK B. Tech Plant Bio-Technology Lab for R13 Batch.

JNTUK B.Tech Plant Bio-Technology Lab gives you detail information of Plant Bio-Technology Lab R13 syllabus It will be help full to understand you complete curriculum of the year.

1. Preparation of Media
2. Surface sterilization
3. Callus induction
4. Organ culture
5. Protoplast isolation
6. Protoplast Culture and Cytological examination
7. Agrobacterium mediated gene transfer
8. Selection of transformants
9. Reporter gene (GUS) assays.

TEXTBOOKS

• Plant Biotechnology: Practical Manual, C. C. Giri & Archana Giri, IK International, 2007.

EQUIPMENTS

1. Autoclave.
2. pH Meter.
3. Laminar air flow chamber.
4. B.O.D. Incubator.

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