JNTUK B.Tech Pharmaceutical Microbiology Lab gives you detail information of Pharmaceutical Microbiology Lab R13 syllabus It will be help full to understand you complete curriculum of the year.
- Study of apparatus used in experimental microbiology.
- Sterilization techniques and their validations.
- Preparation of various culture media.
- Sterilization of glass ware and culture media.
- Aseptic transfer of culture into different types of medias.
- Staining methods – Simple staining, Gram’s staining, Acid fast and negative staining.
- Motility testing by hanging drop method.
- Enumeration of bacteria by pour plate/spread plate technique.
- Enumeration of bacteria by direct microscopic count.
- Isolation of pure cultures by streak plate, spread plate, pour plate.
- Evaluation of antiseptics and disinfectants, sterility of pharmaceutical products as per IP requirements.
- Observation of colony characteristics.
- Bio chemical reactions:
(a) Indole test.
(b) Methyl red test.
(c) Vogesproskauer test.
(d) Starch hydrolysis test.
(e) Fermentation of carbohydrates. - Morphology of molds, yeasts.
- Preseravation of microrganisms (slant and stab cultures)
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