JNTUK B.Tech Molecular Biology gives you detail information of Molecular Biology R13 syllabus It will be help full to understand you complete curriculum of the year.
UNIT I: STRUCTURE OF DNA
Detailed structure of DNA, variation from Watson & Crick model, Z- DNA, A & B DNA, Denaturation & melting curves.
LO: To demonstrate the different of forms of DNA.
LA: Assessment by diagrammatic representation.
UNIT-II: DNA REPLICATION
Models of DNA replication: semi conservative Mechanism of DNA replication in E. coli (bi-directional). Mitochondrial (D-loop), Viral DNA (Rolling circle), Single stranded-DNA phages (M13, ØX174), Eukaryotic telomeres and its replication Inhibitors of DNA Replication. Enzymes involved in replication, step by step process.
LO: To study the models of DNA replication.
LA: Assessment on practical ability and diagrammatic representation.
UNIT III: RNA STRUCTURE AND BIOSYNTHESIS
m- RNA, r-RNA, t-RNA structures, Transcription apparatus, RNA polymerases and proteins involved in transcription (initiation, elongation and termination steps). Post transcriptional processing of RNA‘s t-RNA, r-RNA, m-RNA splicing. Inhibitors of transcription.
LO: To demonstrate the different types of genetic material and factors responsible for its duplication and translation.
LA: Assessment by assembly of transcription apparatus of and performing transcription and translation.
UNIT IV: PROTEIN BIOSYNTHESIS
The genetic code and Wobble Hypothesis, Codon usage, Protein synthesis in Prokaryotes.
LO: To study the methodologies involved in protein synthesis.
LA: Assessment by application of genetic code in analysis of proteins after translation.
UNIT V: PROTEIN SYNTHESIS IN EUKARYOTES
Eukaryotic Protein synthesis, differences between prokaryotic and eukaryotic protein synthesis, Post translational modifications. Inhibitors of protein synthesis.
LO: To study the methods involved in synthesis of proteins in eukaryotes.
LA: Assessment by application of genetic code in analysis of proteins after translation.
UNIT VI: MUTAGENESIS
Mutations, spontaneous, induced, lethal, mutagens their types and actions, classification of mutations and their applications. Site-directed mutagenesis and reverse genetics. DNA damage and repair mechanisms. Mutagenicity testing using microbial systems, Ames TEST.
LO: To study the different types of mutations.
LA: Assessment by theoretical ability.
TEXT BOOKS
- David Friefelder Molecular Biology,4th edition Jones and Bartlett Publishing Home, 2002.
- Short Protocolsin Molecular Biology, 2nd edition T.M. Ausubel, Brent, R.E. Kingston, D.D. Moire, J. G. Seidman, J.A. Smith, K. Struhl Green Publication Associates and John Wiley and sons 2006.
REFERENCES
- Lodish, H., Berk A., Zipursky, S. L. Matsudaria, P. Baltimore, D. and Darnell,J.Molecular Cell of Biology,
- 4th edition, W.H. Freeman and Company, 1999.
- William H. Elli OFF Biochemistry and Molecular biology.4th edition Oxford Publications2009.
- Philip Sheeler Donald E. Bianchi Cell and Molecular biology 3rd edition Wiley Publishers.2007
- De Roberti’s E.D. Pi and De Roberti’s Cell and Molecular Biology 7th edition, Saunders college, 1980. II
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