JNTUK B.Tech Microbiology gives you detail information of Microbiology R13 syllabus It will be help full to understand you complete curriculum of the year.
UNIT I: INTRODUCTION TO MICROBIOLOGY
Discovery of microorganisms; Theory of spontaneous generation, Germ theory of diseases; Scope and relevance of microbiology Identification of Microorganisms- A general account .Microbial diversity General characteristics of Bacteria, Achaea and Eubacteria. Diversity classification by Woese
Classification systems- Phylogenetic, Phenetic, Taxonomic ranks, Major characteristics used in Taxonomy -Morphological, Physiological, ecological, Biochemical, Immunological, Genetical and Molecular.
LO: To demonstrate the isolation & identification of microorganism. LA: Assessment by isolation experiments.
UNIT II: VIRUSES
General properties of Virus , Structure of Viruses; Animal Viruses; plant Viruses; and Classification of Viruses and Applications of Viruses in Biotech Industry, Viral Replication- Bacterial, plant and animal viruses with1example each.
LO: To study the structure classification of virus. LA: Assessment by written test.
UNIT III: ISOLATION, IDENTIFICATION AND CULTURING OF VIRUSES
Isolation, Identification and in vitro cultivation of viruses . Assay of viruses (Both Bacterial and animal viruses.
LO: To demonstrate virus and their isolation.
LA: Assessment by practical demonstration of viral isolation.
UNIT IV: NUTRITION AND CULTIVATION
Nutrition of microorganisms; Nutritional classes of microbes, Macro and micro nutrients, their sources and physiological functions. Growth factors and their functions in metabolism .Aerobic and anaerobic metabolism. Cultivation of microorganisms; Culture media, synthetic, complex media, solidifying agents, types of media- selective, Differential and enrichment and enriched media, pure culture methods- spread plate, pour plate and streak plate, special techniques for cultivation of anaerobes.
LO: To demonstrate the different microbial culturing methods and their requirements.
LA: Assessment by practice demonstration of microbial culturing techniques and media preparation.
UNIT V: IDENTIFICATION AND PRESERVATION OF MICROBES
Preservation of Microganisms: Working and Primary Stock cultures –agar slants, agar stabs, spore preparation, use of sterile soil, cryopreservation, lyophilisation, Application and limitations of various methods. Influence of environmental factors on growth– solutes, water activity, pH, temperature, oxyzen, osomotic pressure, radiation. Colony characteristics, staining techniques; Fixation, Principle dyes, simple standing, differential staining spore staining flagellar staining. Biochemical tests –Sugar fermentations, IMVIC tests, Catalase production etc.
LO: To demonstrate various identification and preservation techniques of micro-organisms
LA: Assessment by demonstration of different techniques of preservation and maintenance of micro-organisms
UNIT VI: MEDICAL BIOTECHNOLOGY
Disease causing microorganisms, Molecular Basis of pathogenecity and identification methods (AIDS, Hepatitis, Polio, Tuberculosis, Anthrax, Cholera).
LO: To study the pathogenesis of micro organism. LA: Assessment by practices and case studies.
TEXTBOOKS
- Pelczar M.J.Chan ECE and Krieg Microbiology, NR.5th edition Tata Mc Graw Hill, 2004.
- John. L. Ingraham, Catherine AL Ingraham Introduction to Micro Biology a case History approach 3rd edition, Thomson Publications, 2000
REFERENCES
- BROCK Biology of Microorganism, 13th edition Prentice Hall, International Inc.2010
- Hans. G. Schlege General Microbiology, 7th edition, Cambridge university press, 1993.
- Roger Y stanier General Microbiology.,5th edition Macmillan.2009
- Prescott and Dunn General Microbiology. 8th edition McGraw Hill Publishers. 2008
For more information about all JNTU updates please stay connected to us on FB and don’t hesitate to ask any questions in the comment.