JNTUK B.Tech Metabolic Engineering gives you detail information of Metabolic Engineering R13 syllabus It will be help full to understand you complete curriculum of the year.
UNIT I: INTRODUCTION IDENTIFICATION OF METABOLIC REGULATION IS A KEY POINT IN METABOLIC ENGINEERING
Basic concepts of Metabolic Engineering –Overview of cellular metabolism–Different models for cellular reactions, induction – Jacob Monod model and its regulation, Differential regulation by isoenzymes, Feedback regulation.
LO: To understand engineering of various metabolic pathways and analyzing its regulation by various methods.
LA: Assessment by modeling engineering of metabolic pathway and demonstrating its regulation.
UNIT II: SYNTHESIS OF PRIMARY METABOLITES AND SECONDARY METABOLITES
Amino acid synthesis pathways and its regulation at enzyme level and whole cell level, Alteration of feedback regulation, Limiting accumulation of end products. Regulation of secondary metabolite pathways, precursor effects, prophase, idiophase relationship, Catabolite regulation by passing control of secondary metabolism, producers of secondary metabolites, applications of secondary metabolites.
LO: To understand the synthesis of amino acids and secondary metabolites and its regulation.
LA: Assessment by demonstrating secondary metabolite synthesis & regulation at cellular and enzymatic level.
UNIT III: BIOCONVERSIONS
Applications of Bioconversions, Factors affecting bioconversions, Specificity, Yields, Co metabolism, Product inhibition, mixed or sequential bioconversions, Conversion of insoluble substances.
LO: To demonstrate basic concepts on bioconversions and factors affecting bioconversions.
LA: Assessment by ability to differentiate different types of bioconversions and factors affecting it.
UNIT IV: REGULATION OF ENZYME PRODUCTION
Strain selection, Genetic improvement of strains, Gene dosage, metabolic pathway manipulations to improve fermentation, Feedback repression, Catabolite Repression, optimization and control of metabolic activities. The modification of existing-or the introduction of entirely new – metabolic pathways
LO: To study the requirements for regulation of enzyme production by microorganism.
LA: Assessment by developing a metabolic pathway by optimizing microorganism.
UNIT V: METABOLIC FLUX
Integration of anabolism and catabolism, metabolic flux distribution analysis, material balance, kinetic types, equilibrium reactions. Experimental determination method of flux distribution, metabolic flux analysis and its applications, Thermodynamics of cellular processes
LO: To study basics concepts metabolic flux analysis
LA: Assessment by ability to implement the flux analysis for product development.
UNIT VI: METABOLIC ENGINEERING WITH BIOINFORMATICS AND ITS APPLICATIONS
Metabolic pathway modeling, Analysis of metabolic control and the structure metabolic networks, Metabolic pathway synthesis algorithms, Application in pharmaceuticals (production of polyketoids), chemical bioprocess (online metabolic flux analysis), food technology (production of golden rice), agriculture (development of draught resistance), environmental bioremediation (development of super bugs) and biomass conversion(production of Ethanol).
LO: To study the engineering of metabolic pathway computationally and its application in different fields.
LA: Assessment by implementing the modeled pathways in different fields.
TEXT BOOKS
- Wang. D. I. C, Cooney, C. L. Demain, A. L. Dunnil, P. Humphrey A. E. Lilly M. D. Fermentation and Enzyme Technology, John Wiley and sons, 1980.
- Stanbury P. F, and Whitaker. A, Principles of Fermentation Technology, Pergamon Press, 1984.
REFERENCES
- Zubay G, Biochemistry, Macmillan Publishers, 1989.
- G. Stephanopoulos, A. Aristidou and J. Nielsen, Metabolic Engineering Principles and Methodologies, Academic Press, 1998.
- Daniel I. C. Wang, Malcolm D. Lilly, Arthur E. Humphrey, Peter Dunnill, Arnold l.Demain, Fermentation and Enzyme Technology,1st edition John Wiley& Sons, Reprint, 2005.
- Christina Smolke, The Metabolic Pathway Engineering Handbook (Two Volume) Set 1st edition CRC press, 2009
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