JNTUK B.Tech Instrumental Methods of Analysis gives you detail information of Instrumental Methods of Analysis R13 syllabus It will be help full to understand you complete curriculum of the year.
UNIT I: INTRODUCTION
Types of Analytical Methods – Instruments for Analysis – Uncertainties in Instrumental measurements – Sensitivity and detection limit for instruments. Microscopy: Bright field, Dark field, Phase contrast, confocal microscopy, SEM & TEM Microscopy, Flow Cytometry.
LO: To understand the basic principles of microscope and their working.
LA: By correct adjustment of slides under different types of microscopes.
UNIT II: CENTRIFUGATION
General Principals, Ultra Centrifugation, velocity Sedimentation & measurements, Equilibrium Ultracentrifugation – Density Gradient centrifugation.
LO: To demonstrate the types of centrifuge and principles.
LA: By separating various sampling using different types of centrifuges.
UNIT-III: SEPARATION EQUIPMENTS – PRINCIPLES AND OPERATION
HPLC, Gas chromatography, Ion – exchange Chromatography, Gel filtration Chromatography, Affinity Chromatography, Membrane separations, Ultra filtration, Reverse Osmosis.
LO: To understand the principle and operation of different types of separation equipment’s.
LA: By separating various sampling using different types of chromatography.
UNIT IV : SPECTROSCOPY
General principles – Radiation, energy and atomic structure- types of spectra and their biochemical usefulness – basic Laws of light absorption. Electromagnetic radiation & Spectrum, Beer – lambert’s law and apparent deviations; UV – VIS Spectrophoto meter, Spectro fluorimetry, Atomic absorption & Atomic emission spectroscopy, Cirular Dichroism (CD)- principles, instrumentation and applications. Infra Red Spectroscopy. Mass spectroscopy-Introduction, analysis, applications in biology ESR principles -instrumentation-applications
LO: To study different types & principle spectrophoto meters
LA: practical analysis of various samples by using different types of spectrophoto meters
UNIT V: ONLINE MONITORING AND CONTROL DEVICES
pH, temperature, dissolved oxygen, agitation, sensors and their operation. Principle, Mode of Operation and Applications.
LO: To study different types & principle of monitoring devices.
LA: by measuring & monitoring of various samples using these devices
UNIT VI: NMR
High resolution NMR– Chemical shift- Spin- spin coupling Frequency lock- double resonance-applications of proton NMR-quantitative analysis- qualitative analysis, application of NMR in biology and study of macromolecules
LO: To study the different principles in NMR.
LA: assessment by s tructure analysis.
TEXTBOOKS
- Keith Wilson, Kenneth H.Goulding A Biologist Guide to principles and techniques of practical Biochemistry. 7th edition.ELBS Series.2010
- Skoog &West, Fundamentals of Analytical Chemistry,8th edition2004
REFERENCES
- Vogel, Text Book of Quantitative Inorganic Analysis, 6th Prentice Hall edition 2000
- Ewing, Instrumental Methods of Analysis, 3rd Mcgraw-Hill College edition 2003
- Hobert H WilLArd D.L. Merritt & J. R. J. A. Dean, Instrumental Methods of Analysis,7th edition CBS Publishers & Distributors,2003
- F. Settle. Hand bookof Instrumental techniques for Analytical Chemistry, Prentice Hall.1997
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