JNTUK B.Tech Genetic Engineering Lab gives you detail information of Genetic Engineering Lab R13 syllabus It will be help full to understand you complete curriculum of the year.
- Isolation of Plant and Bacterial Genomic DNA
- Isolation of Plasmid DNA.
- Visualization of Genomic and plasmid DNA by Agarose gel Electrophoresis.
- Restriction Enzyme digestion.
- DNA Ligation.
- Transformation, screening for recombinants.
- Silver staining of protein gels.
- Blotting Technique.
- Gene Expression of Beta-galactosidase and assay.
- Cloning of DNA into plasmid vector.
TEXT BOOKS
- Maniatis, Current protocols in Molecular Biology, Wiley publications, 2013.
EQUIPMENTS
- Autoclave.
- Laminar air flow chamber. Water bath
- Balance Microfuge. Micropipettes
- Submarine gel electrophoresis unit with power pack. U. V. Transilluminator.
- Vertical slab gel electrophoresis equipment.
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