JNTUK B.Tech Genetic Engineering gives you detail information of Genetic Engineering R13 syllabus It will be help full to understand you complete curriculum of the year.
UNIT I: GENE REGULATION AND EXPRESSION IN PROKARYOTES & EUKARYOTIC SYSTEM
Structure and Functions of Lactose, Arabinose and Tryptophan operons, Sigma switch in Bacillus subtilis. Repressors and activator. Gene regulation in Eukaryotic system, Repetitive DNA, Gene rearrangement, Promoters, enhancer elements, gene amplification.
LO: To understand the Gene Regulation and Expression in Prokaryotes & Eukaryotic system.
LA: Assessment by interpretation of different gene regulations.
UNIT II: PLASMIDS, TRANSPOSONS / VECTORS FOR GENE TRANSFERS
Plasmids: Definition, types, Identification, classification and purifications and transfer of Plasmids. Host restriction in transfer. Transposable elements: Definition, detection of transposition in bacteria, types of bacterial transposons, mechanisms of transposition (Conservative & Replicative) and applications of transposons, Retrotransposons.
LO: To study types, Identification, classification and transfer of Plasmids, transposons.
LA: Assessment by demonstration of different plasmids and its applications.
UNIT III: DNA TECHNOLOGY
Purification of genomic DNA from living cells, Manipulation of purified DNA; construction of prototype vector (pBR322), different types of cloning vectors (plasmid – pUC 19, M13, Lambda phage, cosmid,). Enzymes involved in genetic engineering: cloning strategies, Introduction of DNA into living cells. Methods of Gene transfer, Restriction mapping.
LO: To understand the cloning of DNA and gene transfer methods.
LA: Assessment by applying different cloning and gene transfer strategies.
UNIT IV: EXPRESSION AND DETECTION OF CLONES
Detection of clones and its expression: Expression of cloned genes in yeast & E. coli. Nucleic acid hybridization -Southern, Northern dot blot. DNA methylation. Genomic and cDNA library construction and application. DNA sequencing. Molecular markers: RFLP, RAPD, AFLP, 16s r-RNA typing, gene chip and micro array; SNPs.
LO: To study the Expression and Detection of clones and different molecular markers.
LA: Assessment by practically applying these markers for detection of clones.
UNIT V: PCR AND ITS APPLICATION
Principles, designing of primers, PCR methodology, Reverse Transcriptase PCR & Real Time PCR, multiplex PCR, identification of PCR product, application of PCR technology.
LO: To demonstrate the principle and methodologies of PCR.
LA: Assessment by amplifying different genes using PCR.
UNIT VI: APPLICATIONS OF r-DNA TECHNOLOGY
Gene cloning in medicine (Insulin, Human Growth Hormone) High level expression of proteins in different host systems (Insect, mammalian cells) Limitation and advantages and novel technologies – for generation of transgenic animals. Introduction to Gene therapy (Ex vivo & In vivo), case study of ADA as an example. Advantages and limitations of Gene therapy, gene silencing applications in Cancer.
LO: To study the various applications of gene cloning and gene therapy.
LA: Assessment by application of gene cloning and gene therapy principles in medicine.
TEXT BOOKS
- Old RW, Primrose SB, principles of Gene manipulation, An introduction to Genetic engineering, 6th edition Blackwell Scientific Publications, 2002.
- T. A. Brown, Gene Cloning 6th edition, Wiley-Blackwell Publications, 2010.
- David Frifielder Molecular Biology, 4th edition Jones and Bartlett Publishing Home, 2002. 4. Glick & Pastenick – Molecular Bio-Technology, 4th edition ASM Press, 2009.
REFERENCES
- Ansubel FM., Brent A, Kingston AE, Moore DO, Current protocols in Molecular Biology, Greene Publishing Associates,
NY, 2006. - Berger SL, Kimmer AR, Methods in Enzymology, Vol 152, Academic Press, 1987. 3. Gerald Carp, Molecular Cell Biology, 6th edition Wiley publishers, 2010.
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