JNTUK B.Tech Enzyme Engineering gives you detail information of Enzyme Engineering R13 syllabus It will be help full to understand you complete curriculum of the year.
UNIT I: INTRODUCTION TO ENZYMES
Nomenclature and Classification and application of Enzymes in various sectors – pharmaceutical, food, medical and leather industries (two examples of each); Comparison of chemical and enzyme catalysis.
LO: To demonstrate the classification and applications of enzymes.
LA: Assessment by application of enzymes for productions.
UNIT II: ISOLATION OF ENZYMES
Extraction and Purification of Enzymes from Crude extracts from plant, animal and microbial sources-some case studies, methods of characterization of enzymes, development of enzyme assays.
LO: To understand the isolation of enzymes and different enzyme assays.
LA: Assessment by ability to extract enzymes from different sources.
UNIT III: MECHANISMS AND KINETICS OF ENZYME ACTION
Mechanisms of Enzyme Action- Concept of active site and energetics of enzyme substrate complex formation, Specificity of enzyme action, Kinetics of single substrate reactions, turnover number, estimation of Michaelis-Menton’s parameters. Importance Reactions, Formulation of dimensionless groups and calculation of Effectiveness Factors.
LO: To demonstrate the mechanisms and kinetics of enzymes.
LA: Assessment by deriving the kinetics.
UNIT IV: ENZYME INHIBITION AND IMMOBILIZATION
Types of Inhibition- kinetic models; Substrate and Product Inhibition; Allosteric regulation of enzymes; Deactivation kinetics. Physical and Chemical techniques for enzyme Immobilization – adsorption. Matrix entrapment, encapsulation. Cross-linking. Covalent binding – examples; Advantages and disadvantages of different Immobilization techniques. Overview of applications of immobilized enzyme systems.
LO: To understand the types of immobilization techniques and enzyme inhibition.
LA: Assessment by demonstration of inhibitions.
UNIT V: ENZYME REACTORS
Design of Immobilized Enzyme Reactors-Packed- bed, Fluidized-bed Membrane reactors; Bioconversion calculations in free- enzyme CSTRs and immobilized enzyme reactors.
LO: To demonstrate different types of immobilized enzyme reactors
LA: Assessment by designing of different immobilized reactors.
UNIT VI: ENZYME BIOSENSORS
Applications of enzymes in analysis; Design of enzyme electrodes and their applications as biosensors in industry, health care and environment.
LO: To study design of enzyme electrodes and biosensors.
LA: Assessment by designing of sensors and application of enzyme electrodes.
TEXT BOOKS
- Trevor palmer, Enzymes, 2nd Edition, Wood head Publishing, 2007.
- James E Bailey, David F. Olli’s Biochemical engineering fundamentals, 2nd edition, McGraw Hill Intl, 1986.
REFERENCES
- Colin Ratledge and Bjorm Kristiansen, Basic Biotechnology, 2nd Edition, Cambridge University Press, 2001.
- James Lee, Biochemical Engineering, 1stedition, Prentice Hall, 1991.
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