JNTUK B.Tech Downstream Processing gives you detail information of Downstream Processing R13 syllabus It will be help full to understand you complete curriculum of the year.
UNIT I: ROLE OF DOWNSTREAM PROCESSING IN BIOTECHNOLOGY
Role and importance of downstream processing in biotechnological processes. Problems and requirements of bio product purification. Economics of downstream processing in Biotechnology, cost-cutting strategies, characteristics of biological mixtures, process design criteria for various classes of bioproducts (high volume, low value products and low volume, high Value products).
LO: To develop a detailed strategy about downstream processing.
LA: Assessment by technical applications of downstream processing methods.
UNIT II: PRIMARY SEPARATION AND RECOVERY PROCESS
Cell disruption methods for intracellular products, removal of insoluble, biomass (and particulate debris) separation techniques, flocculation and sedimentation, centrifugation, precipitation and filtration methods.
LO: To understand basics of cell separation and disruption process
LA: Assessment by usage of different membrane and disruption process in cell.
UNIT III: SEPARATIONS AND FRACTIONATION TECHNIQUES
Membrane-based separations (micro and ultrafiltration), theory, design and configuration of membrane separation equipment applications, Electrophoresis of proteins and nucleic acids, 1D-2D Gels, Types of Electrophoretic techniques (Capillary and Pulse field). Chromatographic techniques- Paper, TLC, Adsorption, Ion exchange, Gel filtration, affinity chromatographic separation processes, GC, HPLC, FPLC, Chromatofocusing, electrophoretic separations.
LO: To demonstrate the various separation and fractionation techniques.
LA: Assessment by ability to perform various separation and fractionation techniques.
UNIT IV: BIO-SEPARATION
Physio-chemical basis of bio-separation processes. Recent developments in product Isolation (for ex: one step purification, reverse micellar extraction and online membrane separations).
LO: To understand various physiochemical properties of bio-separation processes.
LA: Assessment by practical application of separation principle based on the sample.
UNIT V: ENRICHMENT OPERATIONS
Precipitation methods (with salts, organic solvents, and polymers, extractive separations, aqueous two-phase extraction, supercritical extraction), in situ product removal, integrated bioprocessing.
LO: To understand various precipitation methods.
LA: Assessment by precipitation of given sample by mentioned methods.
UNIT VI: NEW AND EMERGING TECHNOLOGIES
Dialysis, Crystallization, Evaporation, super liquid extraction foam based separation case study with examples for processing of Two Industrial Products (Citric acid / Penicillin and Low volume high value product like recombinant proteins).
LO: To familiarize new emerging technologies.
LA: Assessment by practical application of these techniques and comparing it with old techniques.
TEXT BOOKS
- Wankat PC, Rate-controlled separations, 1st edition, Elsevier, 2005.
- Belter PA and Cussler E. Bioseparations, 1st edition, Wiley 2011.
REFERENCES
- BIOTOL Series, Product Recovery in Bioprocess Technology, 1st edition, VCH, 2004.
- Asenjo J.M. Separation processes in Biotechnology, Marcel Dekkere Inc, 1993.
- M.R.Ladisch, Bioseparation engineering: Principles, Practice and Economics, 1st edition, Wiley Interscience 2001.
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