JNTUK B.Tech Cell Biology and Microbiology Lab gives you detail information of Cell Biology and Microbiology Lab R13 syllabus It will be help full to understand you complete curriculum of the year.
- Identification of Animal, Plant & Bacterial cells.
- Micrometry.
- Differential centrifugation and isolation of Chloroplast &Mitochondria.
- Sterilization techniques (lecture/ demonstrations).
- Preparation of culture media (a) Broth type of media (b) Solid media.
- Culturing of microorganisms: (a) Broth (b) Pure culture techniques: Streak plate, pour plate.
- Isolation and preservation of bacterial culture.
- Identification of microorganisms (a) Staining technique (b) Biochemical testing.
- Antibiotic test- Disc diffusion method, minimum inhibitory concentration.
- Microbiological examination of water.
- Biochemical tests IMVIC test Catalase test Coagulase test Gelatinase test Oxidase test.
- Determination of Bacterial growth by turbidometry/ colorimetry.
- Factors effecting the bacterial growth– effects of temperature of pH.
TEXTBOOKS
- Microbiological and applications, laboratory, Manual in General Microbiology by Benson, McGraw Publications. First edition 2007
- Laboratory manual in microbiology by P. Gunasekharan, New age international Publishers 2009.
- J. G. Cappuccino and N. Sherman, A Laboratory manual, 9thedition, Addison &weslay,2010.
EQUIPMENT
- Bright field microscope.
- Ocular micrometer.
- Stage micrometer.
- Hot air oven.
- Autoclave.
- Antibiotic disc.
- Laminar air flow chamber.
- Bunsen burner.
- Spectrophotometer.
- Incubator and shaker.
- pH Meter.
- Compound microscope.
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