JNTUK B.Tech Bioprocess Engineering Lab gives you detail information of Bioprocess Engineering Lab R13 syllabus It will be help full to understand you complete curriculum of the year.
- ENZYME ISOLATION AND ASSAY OF ENZYMATIC ACTIVITY
Extraction of commercially important enzymes from natural source Development of enzyme assays; quantification of Enzyme activity and specific activity. - ENZYME KINETICS
Estimation of Michaelis- Menten parameters, Effect of pH and temperature on enzyme activity, kinetics of inhibition. - IMMOBILIZED ENZYME REACTION
Techniques of enzyme immobilization- matrix entrapment, ionic and cross linking; column packing; analysis of mass Transfer effects on kinetics of immobilized enzyme reactions; bioconversion studies with immobilized-enzyme packed– Bed reactors. - MICROBIAL CULTURE STUDIES
Growth of microorganisms, estimation of Monod’s parameters. - SCREENING OF PROCESS VARIABLES Plackett- Burman design practice.
- DEMONSTRATION OF REACTOR STUDIES
Batch, fed- batch, and continuous flow reactor analysis and residence time distribution.
TEXTBOOKS
- P. M. Doran, Biochemical process principles,2nd edition Academic Press,2012..
EQUIPMENTS
- Autoclave.
- pH Meter.
- Laminar air Flow chamber.
- Centrifuge.
- Compound microscope.
- Water bath.
- Packed-bed reactor.
- Shaker-incubator.
- Lyophilizer.
- Spectrofluorimeter.
- U.V. Visible spectrophotometer.
- Hot air oven.
- Incubator.
- Petri plates.
- Micropipettes.
- Bioreactor.
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