JNTUK B.Tech Biochemistry Lab gives you detail information of Biochemistry Lab R13 syllabus It will be help full to understand you complete curriculum of the year.
CYCLE I
- Units, Volume & Weight measurements. Concentration units, pH Measurement. Preparation of buffers.
- Qualitative tests for carbohydrates. Estimation of reducing sugars by the Benedict’s method.
- Qualitative tests for Amino Acids, Quantitative method for Amino Acids, Ninhydrin method.
- Protein estimation by Biuret/ Folin / Bradford method.
- Extraction of lipids, Saponification of Fats.
- Estimation of cholesterol.
- Estimation of Nucleic Acids, Precipitation by sodium sulphate, Test for ribose and deoxyribose sugar.
- Extraction of Caffeine from tea leaves.
- Hydrolysis of ester using Papain.
CYCLE II
- Preparation of simple organic compounds involving the following reactions:
- Acetylation: Acetanilide from aniline and aspirin from salicyclic acid
- Nitration: p- Nitroacetanilide from acetanilide.
- Sulphonation: sulphanilic acid from aniline.
- Oxidation: p-benzoquinone one from hydroquinone.
2. Qualitative analysis
- Carboxylic acids
- Phenols
- Aldehydes and ketones
- Esters
TEXTBOOK
- Laboratory Manaual in Biochemistry by J. Jayaraman New age International Publications, 2011.
- K.Wilson &J.Walker, Principles & Techniques of Practical Biochemistry 5th edition. Cambridge University Press, 2000.
Equipment
- Refrigerator
- Centrifuge.
- Boiling water both.
- Calorimeter.
- pH Meter.
- Weighing Balance.
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