JNTUK B.Tech Biochemical Engineering gives you detail information of Biochemical Engineering R13 syllabus It will be help full to understand you complete curriculum of the year.
Learning Objectives
- To have an overview of the basic structure and function of important cell types, RNA and DNA, amino acids and proteins
- To learn about enzyme structure, function and kinetics of enzyme catalyzed reactions
- To learn about immobilization of enzymes, industrial applications and understand immobilized enzyme kinetics
- To learn about the kinetics of cellular growth, models for cellular growth, and thermal death kinetics of cells and spores
- To understand the various metabolic pathways, biosynthesis, transport across cell membranes, end products of metabolism and stoichiometry of cell growth and product formation
- To get acquainted with design and analysis of various bioreactors and also to have an overview about fermentation technology
UNIT-I: Introduction to Microbiology: Biophysics and the cell doctrine, the structure of cells, Important cell types, from nucleotides to RNA and DNA, amino acids into proteins.
UNIT-II: Kinetics of Enzyme catalyzed reaction: The enzyme substrate complex and enzyme action, Simple enzyme kinetics with one and two substrates, other patterns of substrate concentration dependence, Modulation and regulation of enzyme activity, other influences on enzyme activity.
UNIT-III: Immobilized Enzyme technology: Enzyme immobilization, Industrial processes, utilization and regeneration of cofactors, Immobilized enzyme kinetics: Effect of external mass transfer resistance, Analysis of intra-particle diffusion and reaction.
UNIT-IV: Kinetics of cellular growth in batch and continuous culture, Models for cellular growth – Unstructured, structured and cybernetic models, Thermal death kinetics of cells and spores
UNIT-V: Introduction to metabolic pathways, Biosynthesis, Transport across cell membranes, End products of metabolism, Stoichiometry of cell growth and product formation.
UNIT–VI: Design and analysis of Biological reactors: Batch reactors, fed-batch reactors, Enzyme catalyzed reactions in CSTR, CSTR reactors with recycle and cell growth, Ideal plug flow reactors, Sterilization reactors, Sterilization of gases, packed bed reactors using immobilized catalysts. Fermentation technology: Medium formulation, Design and operation of a typical
aseptic, aerobic fermentation process.
UNIT–VII: Transport phenomena in Bioprocess systems: Gas-liquid mass transfer in cellular systems, determination of oxygen transfer rates, overall kLa’ estimates and power requirements for sparged and agitated vessels, scaling of mass transfer equipment, heat transfer.
UNIT – VIII: Downstream Processing: Strategies to recover and purify products; Separation of insoluble products-filtration and centrifugation; cell disruption-mechanical and non-mechanical methods; Separation of soluble products: liquid-liquid extractions, membrane separation (dialysis, ultra filtration and reverse osmosis), chromatographic separation-gel permeation chromatography,
electrophoresis, final steps in purification – crystallization and drying.
Outcomes
The expected outcomes are that the student
- Will become familiar with basic cell structure and biomolecules.
- Understand the basic principles of gene expression, translation, transcription, regulation and protein synthesis, RNA and DNA
- Grasp the mechanisms and energetics of biomolecule and cell conformation and differentiation, ionic transport and cell communication
- Develop a clear picture of what enzymes are, what their functions are and analyses the kinetics of enzyme catalyzed reactions.
- Demonstrate a clear understanding of immobilized enzyme technology and the kinetics involved.
- Apply the above knowledge to the basic analysis and design of bioreactors.
Text Books
- Biochemical Engineering Fundamentals, J.E.Bailey and D.F.Ollis, 2nd Edition, McGraw Hill, 1986.
- Bioprocess Engineering, Michael L. Shuler and Fikret Kargi, 2nd Edition, Prentice Hall, 2002.
Reference books
- Biochemical Engineering, James M.Lee, Prentice-Hall-1992.
- Biochemical Engineering, Aiba, Humphrey and Mells, Academic press, 1973.
- Bioprocess Engineering principles, Pauline M. Doran, Academic Press, 2012.
- Biochemical Engineering, H.W. Blanch and D.S. Clark, Marcel Dekker, 1997.
- Introduction to Biochemical Engineering, D.G.Rao, Tata McGraw-Hill, 2008.
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