JNTUK B.Tech Animal Cell Science Technology gives you detail information of Animal Cell Science Technology R13 syllabus It will be help full to understand you complete curriculum of the year.
UNIT-I BASICS OF ANIMAL CELL AND ITS CULTURING: Structure and organization of an animal cell, Types of animal cell culture – cell culture, organ/tissue culture, organotypic culture and histotypic culture, Equipment’s and materials needed for animal cell culture technology.
LO: To study the basics of animal cells and its culturing methods.
LA: Assessment by ability to organize and culture different animal cells.
UNIT-II ANIMAL CELL CULTURE MEDIUM AND ITS COMPONENTS AND THEIR SIGNIFICANCE: Introduction to the balanced salt solutions and growth medium, Brief discussion on the chemical, physical and metabolic functions of different constituents of culture medium, Role of carbon-di-oxide and role of serum and its supplements in maintaining cells in culture medium, Serum and protein free defined media and their application.
LO: To study different culture media and its components.
LA: Assessment by ability to develop specific media to various animal cells.
UNIT-III BASIC TECHNIQUES OF MAMMALIAN CELL CULTURE in vitro: Primary and established cell lines, Biology and characterization of the cultured cells, measuring parameters of growth. Maintenance of cell culture, Cell separation, Cell transformation, Cell synchronization, Measurement of viability and cytotoxicity, Apoptosis – characteristic features and molecular mechanisms, Measurement of cell death.
LO: To study basic techniques of in vitro culturing techniques of mammalian cells.
LA: Assessment by ability to culture and apply different in vitro culturing techniques of mammalian cells.
UNIT-IV ENGINEERING ANIMAL CELLS: Somatic cell genetics, Cell culture based vaccines, Genetic engineering of mammalian cells in culture, Scaling up of animal cell culture.
LO: To study genetics of various animal cells.
LA: Assessment by ability to produce cell culture based vaccines and the scale up of cell cultures.
UNIT-V STEM CELLS
Definition; Types- Pleuripotent v/s Totipotent; Embryonic, Adult and Fetal; Role of Stem cells in Tissue Engineering; Application of Stem cells.
LO: To study the definition, role and applications of stem cells.
LA: Assessment by ability to apply the concept of stem cells in various fields.
UNIT-VI APPLICATIONS OF ANIMAL CELL CULTURE:
Three dimensional culture and tissue engineering, Applications of animal cell culture technology (heterologous, Primary culture/CEF culturing, Protein Expression).
LO: To study the applications of animal cell cultures.
LA: Assessment by ability to apply animal cell culturing in protein engineering.
TEXT BOOKS
- Ian Froshney, Culture of Animal Cells, 3 rd Ed, Wiley-Liss 2001.
- Animal Cell Culture Techniques – Practical Approach, Ed. John R.W. Masters Oxford 2003.
REFERENCES
- Cell Cultures lab fax. Eds. M. Butter & M. Dawson, Bios Scientific publications Ltd ., Oxford 2003.
- Animal Cell Cultures Techniques. Ed. Martin Clynes, Springer 2001.
- Methods in Cell Biology, Vol. 57, Animal Cell Culture Methods. Ed. Jenni P. Mather and David Bares. Academic Press 2003.
- Cell Growth and Division: A practical Approach. Ed. R. Basega, IRLpress 2001.
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