JNTUH B.Tech 2nd year Microbiology gives you detail information about Microbiology Engineering R13 year subject. It will be help full you to understand you complete curriculum of the year.<\/p>\n
Objectives<\/strong><\/p>\n This course is formulated to provides an exposure to microbial diversity, their characteristics,\u00a0classification, physiology and identification. It also enables them to understand host pathogen interaction and\u00a0their microbial environment. It also introduces the students to pathogens and viruses.<\/p>\n UNIT I:\u00a0INTRODUCTION TO MICROBIOLOGY<\/strong><\/p>\n Discovery of micro organisms; Theory of spontaneous generation, Germ\u00a0theory of diseases; Major contribution and events in the field of Microbiology. Scope and relevance of\u00a0microbiology.\u00a0Identification of Micro organisms – A general account. Micro diversity<\/p>\n UNIT II:\u00a0MAJOR GROUPS OF MICRO ORGANISMS AND GENERAL METHODS OF IDENTIFICATION<\/strong><\/p>\n General\u00a0characteristics of Bacteria, Archaea and Eubacteria. Diversity classification of Woese et al. Three domains of life.\u00a0Five – kingdom system of Whittaker.\u00a0Classification systems – Phylogenetic, Phenetic, Taxonomic ranks, Major characteristics used in Taxonomy –\u00a0Morphological, Physiological, ecological, Biochemical, Immunological, Genetic and Molecular.\u00a0Colony characteristics, staining techniques; Fixation, Principle dyes, simple staining, differential staining, spore\u00a0staining, flageller staining.\u00a0Biochemical tests \u2013 Sugar fermentations, IMVIC tests, Catalase production etc.<\/p>\n UNIT III:\u00a0NUTRITION, CULTIVATION AND PRESERVATION OF MICROORGANISMS<\/strong><\/p>\n Nutrition requirements of\u00a0micro organisms; nutritional classes of microbes, Macro and micro nutrients, their sources and physiological\u00a0functions of nutrients. Growth factors and their functions in metabolism. Aerobic and anaerobic metabolism.
\nCultivation of micro organisms; Culture media, synthetic, complex media, solidifying agents, types of media –\u00a0selective, differential and enrichment and enriched media, pure culture methods – spread plate, pour plate and\u00a0streak plate, special techniques for cultivation of anaerobes.\u00a0Influence of environmental factors on growth \u2013 solutes, water activity, pH, temperature, oxyzen, osomotic\u00a0pressure, radiation.\u00a0Preservation of Microganisms: working and primary stock cultures \u2013 agar slants, agar stabs, spore preparation,\u00a0use of sterile soil, cryopreservation, lyophilisation, Application and limitations of various methods.<\/p>\n